Elongation factor TFIIS is essential for heat stress adaptation in plants
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- István Szádeczky-Kardoss
- Genetics and Biotechnology Institute, MATE University, Szent-Györgyi A. u. 4, 2100 Gödöllő, Hungary
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- Henrik Mihály Szaker
- Genetics and Biotechnology Institute, MATE University, Szent-Györgyi A. u. 4, 2100 Gödöllő, Hungary
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- Radhika Verma
- Genetics and Biotechnology Institute, MATE University, Szent-Györgyi A. u. 4, 2100 Gödöllő, Hungary
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- Éva Darkó
- Agricultural Institute, Centre for Agricultural Research, Brunszvik u. 2., 2462 Martonvásár, Hungary
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- Aladár Pettkó-Szandtner
- Proteomics Laboratory, Biological Research Centre, Temesvári krt. 62., 6726 Szeged, Hungary
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- Dániel Silhavy
- Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., 6726 Szeged, Hungary
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- Tibor Csorba
- Genetics and Biotechnology Institute, MATE University, Szent-Györgyi A. u. 4, 2100 Gödöllő, Hungary
Description
<jats:title>Abstract</jats:title> <jats:p>Elongation factor TFIIS (transcription factor IIS) is structurally and biochemically probably the best characterized elongation cofactor of RNA polymerase II. However, little is known about TFIIS regulation or its roles during stress responses. Here, we show that, although TFIIS seems unnecessary under optimal conditions in Arabidopsis, its absence renders plants supersensitive to heat; tfIIs mutants die even when exposed to sublethal high temperature. TFIIS activity is required for thermal adaptation throughout the whole life cycle of plants, ensuring both survival and reproductive success. By employing a transcriptome analysis, we unravel that the absence of TFIIS makes transcriptional reprogramming sluggish, and affects expression and alternative splicing pattern of hundreds of heat-regulated transcripts. Transcriptome changes indirectly cause proteotoxic stress and deterioration of cellular pathways, including photosynthesis, which finally leads to lethality. Contrary to expectations of being constantly present to support transcription, we show that TFIIS is dynamically regulated. TFIIS accumulation during heat occurs in evolutionary distant species, including the unicellular alga Chlamydomonas reinhardtii, dicot Brassica napus and monocot Hordeum vulgare, suggesting that the vital role of TFIIS in stress adaptation of plants is conserved.</jats:p>
Journal
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- Nucleic Acids Research
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Nucleic Acids Research 50 (4), 1927-1950, 2022-01-31
Oxford University Press (OUP)
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Details 詳細情報について
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- CRID
- 1360861710910548352
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- ISSN
- 13624962
- 03051048
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- Data Source
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- Crossref