Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis

DOI XML XML 1 Citations
  • S. Stanisavljević
    Department of Immunology, Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia
  • J. Lukić
    Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia
  • M. Miljković
    Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia
  • M. Kojić
    Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia
  • B. Jevtić
    Department of Immunology, Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia
  • N. Golić
    Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia
  • M. Mostarica Stojković
    Institute for Microbiology and Immunology, School of Medicine, University of Belgrade, dr. Subotica 1, 11000 Belgrade, Serbia
  • D. Miljković
    Department of Immunology, Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia
  • M. Momčilović
    Department of Immunology, Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Despota Stefana 142, 11000 Belgrade, Serbia

Bibliographic Information

Published
2016-06-01
DOI
  • 10.3920/bm2015.0159
Publisher
Walter de Gruyter GmbH

Search this article

Description

<jats:p>Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund’s adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer’s patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4<jats:sup>+</jats:sup>T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-γ and interleukin-17. Finally, microbial analyses showed that uncultivated species of<jats:italic>Turicibacter</jats:italic>and<jats:italic>Atopostipes</jats:italic>genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.</jats:p>

Journal

Citations (1)*help

See more

Report a problem

Back to top