{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361131418281262720.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41598-019-45331-8"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-019-45331-8.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-019-45331-8"}},{"identifier":{"@type":"PMID","@value":"31222045"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Soybean (Glycine max L.) triacylglycerol lipase GmSDP1 regulates the quality and quantity of seed oil"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Seeds of soybean (<jats:italic>Glycine max</jats:italic> L.) are a major source of plant-derived oils. In the past, improvements have been made in the quantity and quality of seed oil. Triacylglycerols (TAGs) are the principal components of soybean seed oil, and understanding the metabolic regulation of TAGs in soybean seeds is essential. Here, we identified four soybean genes encoding TAG lipases, designated as <jats:italic>SUGAR DEPENDENT1-1</jats:italic> (<jats:italic>GmSDP1-1</jats:italic>), <jats:italic>GmSDP1-</jats:italic>2, <jats:italic>GmSDP1-3</jats:italic> and <jats:italic>GmSDP1-4</jats:italic>; these are homologous to <jats:italic>Arabidopsis thaliana SDP1</jats:italic> (<jats:italic>AtSDP1</jats:italic>). To characterize the function of these genes during grain filling, transgenic lines of soybean were generated via RNA interference to knockdown the expression of all four <jats:italic>GmSDP1</jats:italic> genes. The seed oil content of the transgenic soybean lines was significantly increased compared with the wild type (WT). Additionally, fatty acid profiles of the WT and transgenic soybean lines were altered; the content of linoleic acid, a major fatty acid in soybean seeds, was significantly reduced, whereas that of oleic acid was increased in transgenic soybean seeds compared with the WT. Substrate specificity experiments showed that TAG lipase preferentially cleaved oleic acid than linoleic acid in the oil body membrane in WT soybean. This study demonstrates that the GmSDP1 proteins regulate both the TAG content and fatty acid composition of soybean seeds during grain filling. These results provide a novel strategy for improving both the quantity and quality of soybean seed oil.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381131418281262603","@type":"Researcher","foaf:name":[{"@value":"Masatake Kanai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381131418281262464","@type":"Researcher","foaf:name":[{"@value":"Tetsuya Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801209121408","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50212155"},{"@type":"NRID","@value":"1000050212155"},{"@type":"NRID","@value":"9000364971842"},{"@type":"NRID","@value":"9000018277074"},{"@type":"NRID","@value":"9000022036861"},{"@type":"NRID","@value":"9000392826841"},{"@type":"NRID","@value":"9000018276839"}],"foaf:name":[{"@value":"Makoto Hayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381131418281262469","@type":"Researcher","foaf:name":[{"@value":"Shoji Mano"}]},{"@id":"https://cir.nii.ac.jp/crid/1381131418281262852","@type":"Researcher","foaf:name":[{"@value":"Mikio Nishimura"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20452322"}],"prism:publicationName":[{"@value":"Scientific Reports"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2019-06-20","prism:volume":"9","prism:number":"1"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41598-019-45331-8.pdf"},{"@id":"https://www.nature.com/articles/s41598-019-45331-8"}],"createdAt":"2019-06-20","modifiedAt":"2022-12-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Glycine%20max","dc:title":"Glycine max"},{"@id":"https://cir.nii.ac.jp/all?q=Fatty%20Acids","dc:title":"Fatty Acids"},{"@id":"https://cir.nii.ac.jp/all?q=Lipase","dc:title":"Lipase"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Plant","dc:title":"Genes, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Plants,%20Genetically%20Modified","dc:title":"Plants, Genetically Modified"},{"@id":"https://cir.nii.ac.jp/all?q=Article","dc:title":"Article"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Plant","dc:title":"Gene Expression Regulation, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Seeds","dc:title":"Seeds"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Oils","dc:title":"Plant Oils"},{"@id":"https://cir.nii.ac.jp/all?q=Triglycerides","dc:title":"Triglycerides"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Proteins","dc:title":"Plant Proteins"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782006761600","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18KT0095"},{"@type":"JGN","@value":"JP18KT0095"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KT0095/"}],"notation":[{"@language":"ja","@value":"耕作放棄地を利用した茶油生産のための施肥管理技術の構築"},{"@language":"en","@value":"Development of Fertilizer Management Technology for Tea Oil Production Using Abandoned Land"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782018524672","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19K06731"},{"@type":"JGN","@value":"JP19K06731"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K06731/"}],"notation":[{"@language":"ja","@value":"プラスチド局在型葉酸によるデンプン合成抑制機構の解明"},{"@language":"en","@value":"Study on mechanism suppressing starch biosynthesis by plastidial folate"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050306506455303168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Knockdown of the 7S globulin subunits shifts distribution of nitrogen sources to the residual protein fraction in transgenic soybean seeds"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145892223488","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Enhanced Seed Oil Production in Canola by Conditional Expression of <i>Brassica napus LEAFY COTYLEDON1</i> and <i>LEC1-LIKE</i> in Developing Seeds"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692628687744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Plastidic DEAD-box RNA Helicase 22, HS3, is Essential for Plastid Functions Both in Seed Development and in Seedling Growth"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618588585856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid β-Oxidation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620264067328","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574092884653696","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Phytozome: a comparative platform for green plant genomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094233760512","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Seed Storage Oil Mobilization Is Important But Not Essential for Germination or Seedling Establishment in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095617103232","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Plant Peroxisomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096250126720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Cytosolic Acyltransferase Contributes to Triacylglycerol Synthesis in Sucrose-Rescued Arabidopsis Seed Oil Catabolism Mutants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096411170304","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Arabidopsis <i>pxa1</i> Mutant Is Defective in an ATP-Binding Cassette Transporter-Like Protein Required for Peroxisomal Fatty Acid β-Oxidation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846643282963584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Extension of oil biosynthesis during the mid‐phase of seed development enhances oil content in <i>Arabidopsis</i> seeds"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846645705153024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855567866361856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genome sequence of the palaeopolyploid soybean"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568750428416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Storage oil breakdown during embryo development of Brassica napus (L.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571362597248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Seed Storage Oil Mobilization"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043924872192","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Increased Flow of Fatty Acids toward β-Oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-Chain-Length Fatty Acids"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044099765120","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Chewing the fat: β-oxidation in signalling and development"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044386435456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A high‐oleic‐acid and low‐palmitic‐acid soybean: agronomic performance and evaluation as a feedstock for biodiesel"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044745829760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"<i>SUGAR-DEPENDENT1</i>\n                    Encodes a Patatin Domain Triacylglycerol Lipase That Initiates Storage Oil Breakdown in Germinating\n                    <i>Arabidopsis</i>\n                    Seeds"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044971478912","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519147203456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biochemical pathways in seed oil synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993735100928","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996193504640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"High‐value oils from plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468450019712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Plant triacylglycerols as feedstocks for the production of biofuels"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469006741376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469297582080","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cloning and Characterization of the Acid Lipase from Castor Beans"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945046738304","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Arabidopsis thaliana—A model organism to study plant peroxisomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945201963776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Understanding and manipulating plant lipid composition: Metabolic engineering leads the way"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262946098153728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Compartmentation of Triacylglycerol Accumulation in Plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419129540736","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Seed storage oil catabolism: a story of give and take"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419524831616","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Expression of Rapeseed Microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419873727872","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Agronomic and Seed Traits of Soybean Lines with High Oleate Concentration"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419982306560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Physiological and developmental regulation of seed oil production"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420057545088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Tailoring plant lipid composition: designer oilseeds come of age"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893501320192","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Suppression of the <i><scp>SUGAR</scp>‐<scp>DEPENDENT</scp>1</i> triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (<i><scp>B</scp>rassica napus </i><scp>L</scp>.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894345779456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"DEVELOPMENT OF GENETICALLY ENGINEERED SOYBEAN OILS FOR FOOD APPLICATIONS"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844854345216","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670321233533824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Expression of <i>ZmLEC1</i> and <i>ZmWRI1</i> Increases Seed Oil Production in Maize"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795300306048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Diacylglycerol acyltransferase: A key mediator of plant triacylglycerol synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269005826560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204328441600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean"},{"@value":"Transgenic note: Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1038/s41598-019-45331-8"},{"@type":"KAKEN","@value":"PRODUCT-22732106"},{"@type":"KAKEN","@value":"PRODUCT-22705030"},{"@type":"OPENAIRE","@value":"doi_dedup___::8ec9e7045704150a2e60d7f41c0fd009"}]}