[Updated on Apr. 18] Integration of CiNii Articles into CiNii Research

CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction

  • Jiang Sun
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Yonggang Lu
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Kaori Nozawa
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Zoulan Xu
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Akane Morohoshi
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Julio M Castaneda
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Taichi Noda
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Haruhiko Miyata
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Ferheen Abbasi
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
  • Hossam H Shawki
    Department of Comparative and Experimental Medicine, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan
  • Satoru Takahashi
    Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Darius J Devlin
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Zhifeng Yu
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Ryan M Matzuk
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Thomas X Garcia
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Martin M Matzuk
    Center for Drug Discovery, Baylor College of Medicine, Houston, Texas, USA
  • Masahito Ikawa
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

Bibliographic Information

Other Title
  • CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction†

Search this article

Abstract

<jats:title>Abstract</jats:title><jats:p>Developing a safe and effective male contraceptive remains a challenge in the field of medical science. Molecules that selectively target the male reproductive tract and whose targets are indispensable for male reproductive function serve among the best candidates for a novel non-hormonal male contraceptive method. To determine the function of these genes in vivo, mutant mice carrying disrupted testis- or epididymis-enriched genes were generated by zygote microinjection or electroporation of the CRISPR/Cas9 components. Male fecundity was determined by consecutively pairing knockout males with wild-type females and comparing the fecundity of wild-type controls. Phenotypic analyses of testis appearance and weight, testis and epididymis histology, and sperm movement were further carried out to examine any potential spermatogenic or sperm maturation defect in mutant males. In this study, we uncovered 13 testis- or epididymis-enriched evolutionarily conserved genes that are individually dispensable for male fertility in mice. Owing to their dispensable nature, it is not feasible to use these targets for the development of a male contraceptive.</jats:p>

Journal

Citations (1)*help

See more

References(58)*help

See more

Related Articles

See more

Related Data

See more

Related Books

See more

Related Dissertations

See more

Related Projects

See more

Related Products

See more

Report a problem

Back to top