{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043477823232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/ismej.2009.132"}},{"identifier":{"@type":"URI","@value":"http://www.nature.com/articles/ismej2009132.pdf"}},{"identifier":{"@type":"URI","@value":"http://www.nature.com/articles/ismej2009132"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/ismej/article-pdf/4/4/531/56402555/41396_2010_article_bfismej2009132.pdf"}},{"identifier":{"@type":"PMID","@value":"19956275"}}],"dc:title":[{"@value":"Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H2-using methanogens in the groundwater. Furthermore, the high potential of methane production by H2-using methanogens was shown in enrichments using groundwater amended with H2 and CO2. Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H2 and CO2 generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H2-producing fermentative bacteria and H2-using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H2 accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 °C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H2-producing fermentative bacteria and H2-using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043477823232","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Kimura"}],"jpcoar:affiliationName":[{"@value":"Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823360","@type":"Researcher","foaf:name":[{"@value":"Hiroaki Nashimoto"}],"jpcoar:affiliationName":[{"@value":"Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823362","@type":"Researcher","foaf:name":[{"@value":"Mikio Shimizu"}],"jpcoar:affiliationName":[{"@value":"Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823233","@type":"Researcher","foaf:name":[{"@value":"Shohei Hattori"}],"jpcoar:affiliationName":[{"@value":"Department of Environmental Science and Technology, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823361","@type":"Researcher","foaf:name":[{"@value":"Keita Yamada"}],"jpcoar:affiliationName":[{"@value":"Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823363","@type":"Researcher","foaf:name":[{"@value":"Keisuke Koba"}],"jpcoar:affiliationName":[{"@value":"Faculty of Agriculture, Tokyo University of Agriculture and Technology , Tokyo , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823234","@type":"Researcher","foaf:name":[{"@value":"Naohiro Yoshida"}],"jpcoar:affiliationName":[{"@value":"Department of Environmental Science and Technology, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043477823364","@type":"Researcher","foaf:name":[{"@value":"Kenji Kato"}],"jpcoar:affiliationName":[{"@value":"Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"17517362"},{"@type":"EISSN","@value":"17517370"}],"prism:publicationName":[{"@value":"The ISME Journal"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2009-12-03","prism:volume":"4","prism:number":"4","prism:startingPage":"531","prism:endingPage":"541"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://academic.oup.com/pages/standard-publication-reuse-rights"],"url":[{"@id":"http://www.nature.com/articles/ismej2009132.pdf"},{"@id":"http://www.nature.com/articles/ismej2009132"},{"@id":"https://academic.oup.com/ismej/article-pdf/4/4/531/56402555/41396_2010_article_bfismej2009132.pdf"}],"createdAt":"2009-12-03","modifiedAt":"2025-02-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Bacterial","dc:title":"DNA, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=Hot%20Temperature","dc:title":"Hot Temperature"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Archaeal","dc:title":"RNA, Archaeal"},{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Ribosomal","dc:title":"DNA, Ribosomal"},{"@id":"https://cir.nii.ac.jp/all?q=Japan","dc:title":"Japan"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Ribosomal,%2016S","dc:title":"RNA, Ribosomal, 16S"},{"@id":"https://cir.nii.ac.jp/all?q=Sequence%20Homology,%20Nucleic%20Acid","dc:title":"Sequence Homology, Nucleic Acid"},{"@id":"https://cir.nii.ac.jp/all?q=Cluster%20Analysis","dc:title":"Cluster Analysis"},{"@id":"https://cir.nii.ac.jp/all?q=Anaerobiosis","dc:title":"Anaerobiosis"},{"@id":"https://cir.nii.ac.jp/all?q=Phylogeny","dc:title":"Phylogeny"},{"@id":"https://cir.nii.ac.jp/all?q=Carbon%20Isotopes","dc:title":"Carbon Isotopes"},{"@id":"https://cir.nii.ac.jp/all?q=Bacteria","dc:title":"Bacteria"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20rRNA","dc:title":"Genes, rRNA"},{"@id":"https://cir.nii.ac.jp/all?q=Sequence%20Analysis,%20DNA","dc:title":"Sequence Analysis, DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Carbon%20Dioxide","dc:title":"Carbon Dioxide"},{"@id":"https://cir.nii.ac.jp/all?q=Archaea","dc:title":"Archaea"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Bacterial","dc:title":"RNA, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Archaeal","dc:title":"DNA, Archaeal"},{"@id":"https://cir.nii.ac.jp/all?q=Water%20Microbiology","dc:title":"Water Microbiology"},{"@id":"https://cir.nii.ac.jp/all?q=Methane","dc:title":"Methane"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen","dc:title":"Hydrogen"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004235460812672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235462535424","@type":"Article","resourceType":"学術雑誌論文(journal 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