{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043564319872.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s12010-014-0814-5"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s12010-014-0814-5.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/s12010-014-0814-5/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s12010-014-0814-5"}},{"identifier":{"@type":"PMID","@value":"24599668"}}],"dc:title":[{"@value":"Expression, Purification, and Characterization of Endo-β-N-Acetylglucosaminidase H Using Baculovirus-Mediated Silkworm Protein Expression System"}],"description":[{"notation":[{"@value":"Endo-β-N-acetylglucosaminidase (Endo H) from Streptomyces plicatus hydrolyzes the core di-GlcNAc units of asparagine-linked oligosaccharides and is regarded as an important tool for glycobiology research. In the present study, we established a large-scale system to produce secreted Endo H using a silkworm-baculovirus expression system (silkworm-BES). The recombinant Endo H purified from silkworm hemolymph had activity comparable to that from recombinant Escherichia coli. As well as its well-characterized substrate RNase B, the Endo H from silkworm-BES was able to deglycosylate the high-mannose glycoproteins from silkworm hemolymph. Interestingly, the secretion amount of recombinant Endo H was significantly varied among the different silkworm strains, which could provide valuable information for larger-scale protein productions from silkworm-BES."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043564319878","@type":"Researcher","foaf:name":[{"@value":"Takumi Mitsudome"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319880","@type":"Researcher","foaf:name":[{"@value":"Jian Xu"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319879","@type":"Researcher","foaf:name":[{"@value":"Yudai Nagata"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319874","@type":"Researcher","foaf:name":[{"@value":"Atsushi Masuda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319877","@type":"Researcher","foaf:name":[{"@value":"Kazuhiro Iiyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319875","@type":"Researcher","foaf:name":[{"@value":"Daisuke Morokuma"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319872","@type":"Researcher","foaf:name":[{"@value":"Zhiqing Li"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801207603840","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30616412"},{"@type":"NRID","@value":"1000030616412"},{"@type":"NRID","@value":"9000260216338"},{"@type":"NRID","@value":"9000260205753"},{"@type":"NRID","@value":"9000260208994"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/mon_hiroaki37"}],"foaf:name":[{"@value":"Hiroaki Mon"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319873","@type":"Researcher","foaf:name":[{"@value":"Jae Man Lee"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043564319744","@type":"Researcher","foaf:name":[{"@value":"Takahiro Kusakabe"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"02732289"},{"@type":"EISSN","@value":"15590291"}],"prism:publicationName":[{"@value":"Applied Biochemistry and Biotechnology"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2014-03-06","prism:volume":"172","prism:number":"8","prism:startingPage":"3978","prism:endingPage":"3988"},"reviewed":"false","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/s12010-014-0814-5.pdf"},{"@id":"http://link.springer.com/article/10.1007/s12010-014-0814-5/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1007/s12010-014-0814-5"}],"createdAt":"2014-03-05","modifiedAt":"2022-03-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Glycosylation","dc:title":"Glycosylation"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression","dc:title":"Gene Expression"},{"@id":"https://cir.nii.ac.jp/all?q=Bombyx","dc:title":"Bombyx"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Proteins","dc:title":"Recombinant Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Mannosyl-Glycoprotein%20Endo-beta-N-Acetylglucosaminidase","dc:title":"Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase"},{"@id":"https://cir.nii.ac.jp/all?q=Larva","dc:title":"Larva"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Engineering","dc:title":"Genetic Engineering"},{"@id":"https://cir.nii.ac.jp/all?q=Baculoviridae","dc:title":"Baculoviridae"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001338872768512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Purification of human papillomavirus-like particles expressed in silkworm using a Bombyx mori nucleopolyhedrovirus bacmid expression system"}]},{"@id":"https://cir.nii.ac.jp/crid/1050013706703162752","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Display of multiple proteins on engineered canine parvovirus-like particles expressed in cultured silkworm cells and silkworm larvae"}]},{"@id":"https://cir.nii.ac.jp/crid/1050286428537737088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body"}]},{"@id":"https://cir.nii.ac.jp/crid/1050845763804090240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms"}]},{"@id":"https://cir.nii.ac.jp/crid/1050845764094336768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Purification of virus-like particles (VLPs) expressed in the silkworm Bombyx mori"}]},{"@id":"https://cir.nii.ac.jp/crid/1050845764199904768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A systematic and methodical approach for the efficient purification of recombinant protein from silkworm larval hemolymph"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004229959881600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Polymerization of Horseradish Peroxidase by a Laccase‐Catalyzed Tyrosine Coupling Reaction"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231986172160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168867849088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017279862125056","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360025430647548160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Efficient and accurate BmNPV bacmid editing system by two-step golden gate assembly"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182136248704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182202756352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional horseradish peroxidase−streptavidin chimeric proteins prepared using a silkworm-baculovirus expression system for diagnostic purposes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360568466077893120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118686779520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848654889966336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression and Activation of Horseradish Peroxidase-Protein A/G Fusion Protein in Silkworm Larvae for Diagnostic Purposes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656916246144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Improvement of Endo-β-N-acetylglucosaminidase H production using silkworm–baculovirus protein expression system"}]},{"@id":"https://cir.nii.ac.jp/crid/1523951029567352704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1007/s12010-014-0814-5"},{"@type":"OPENAIRE","@value":"doi_dedup___::9ea480c50d9a74c6febc0509319ce824"},{"@type":"CROSSREF","@value":"10.1002/biot.201800531_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1007/s10529-018-2516-5_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.jchromb.2019.121964_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.aspen.2021.04.011_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.pep.2022.106096_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.3389/fbioe.2023.1096363_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.jviromet.2024.115029_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1007/s12033-019-00184-4_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.3390/ijms20174228_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2015.02.013_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1186/s12951-020-00715-1_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.3389/fimmu.2021.803647_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1002/biot.201700624_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.ibmb.2015.01.008_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.aspen.2019.01.009_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.jchromb.2018.08.007_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.aspen.2015.01.006_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"},{"@type":"CROSSREF","@value":"10.1016/j.jbiotec.2019.03.007_references_DOI_IjUYCC5boGfN7dONaajwjFnMvyt"}]}