{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043673423488.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/pdb.prot5439"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/pdb.prot5439"}}],"dc:title":[{"@value":"Purification of RNA Using TRIzol (TRI Reagent)"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>INTRODUCTION</jats:title><jats:p>TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical. TRIzol (or TRI Reagent) is a monophasic solution of phenol and guanidinium isothiocyanate that simultaneously solubilizes biological material and denatures protein. After solubilization, the addition of chloroform causes phase separation (much like extraction with phenol:chloroform:isoamyl alcohol), where protein is extracted to the organic phase, DNA resolves at the interface, and RNA remains in the aqueous phase. Therefore, RNA, DNA, and protein can be purified from a single sample (hence, the name TRIzol). TRIzol extraction is also an effective method for isolating small RNAs, such as microRNAs, piwi-associated RNAs, or endogeneous, small interfering RNAs. However, TRIzol is expensive and RNA pellets can be difficult to resuspend. Thus, the use of TRIzol is not recommend when regular phenol extraction is practical.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043673423490","@type":"Researcher","foaf:name":[{"@value":"Donald C. Rio"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043673423491","@type":"Researcher","foaf:name":[{"@value":"Manuel Ares"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043673423489","@type":"Researcher","foaf:name":[{"@value":"Gregory J. Hannon"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043673423488","@type":"Researcher","foaf:name":[{"@value":"Timothy W. Nilsen"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"19403402"},{"@type":"EISSN","@value":"15596095"},{"@type":"PISSN","@value":"15596095"}],"prism:publicationName":[{"@value":"Cold Spring Harbor Protocols"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2010-06","prism:volume":"2010","prism:number":"6","prism:startingPage":"pdb.prot5439"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/pdb.prot5439"}],"createdAt":"2010-06-01","modifiedAt":"2021-11-10","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142739595392","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibition of fibrotic changes in infrapatellar fat pad alleviates persistent pain and articular cartilage degeneration in monoiodoacetic acid-induced rat arthritis model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168813857792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Establishment of a patient‑derived mucoepidermoid carcinoma cell line with the <i>CRTC1‑MAML2</i> fusion gene"}]},{"@id":"https://cir.nii.ac.jp/crid/1360025431095732608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Altered neutrophil responses to dengue virus serotype three: delayed apoptosis is regulated by stabilisation of Mcl-1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617807088256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dynamic polarization shifting from M1 to M2 macrophages in reduced osteonecrosis of the jaw-like lesions by cessation of anti-RANKL antibody in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092392542720","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Suppression of circadian clock protein cryptochrome 2 promotes osteoarthritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092483421824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Boron‐dependent regulation of translation through AUGUAA sequence in yeast"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204370789376","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparison of commercial RNA extraction kits and qPCR master mixes for studying gene expression in small biopsy tissue samples from the equine gastric epithelium"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206478573056","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Extraction of High-Quality RNA from Rubber Tree Leaves"}]},{"@id":"https://cir.nii.ac.jp/crid/1390852669109220352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Gene expression analysis of fresh extraction wounds prior to onset of bisphosphonate-related osteonecrosis of the jaw-like lesions in mice: A preliminary animal study"}]},{"@id":"https://cir.nii.ac.jp/crid/1520857435091911808","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Efficient DNA/RNA extraction from tarsal plates by SK mill, a freeze-crush apparatus"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1101/pdb.prot5439"},{"@type":"CROSSREF","@value":"10.1016/j.joca.2020.12.014_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.3892/mco.2022.2508_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1038/s41598-024-68642-x_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1016/j.bone.2020.115560_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1271/bbb.120014_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1016/j.joca.2020.04.004_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1002/yea.3526_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.2186/jpr.jpr_d_20_00027_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1093/jb/mvac078_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"},{"@type":"CROSSREF","@value":"10.1294/jes.28.135_references_DOI_W6vPxNXEN5bqcIlxTbhvRm73dvK"}]}