{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043683653376.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1182/blood.v98.2.414"}},{"identifier":{"@type":"URI","@value":"http://ashpublications.org/blood/article-pdf/98/2/414/1675146/h8140100414.pdf"}},{"identifier":{"@type":"NAID","@value":"30022493445"}}],"dc:title":[{"@value":"Apaf-1 protein deficiency confers resistance to cytochromec–dependent apoptosis in human leukemic cells"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The human leukemia cell lines K562, CEM, CEM/VLB100, human leukemic blasts, and the bladder cancer J82 cell line have different sensitivities to UV light–induced apoptosis. It is reported that resistance to UV light–induced apoptosis occurs at a point in the apoptotic pathway upstream of caspase-3 but downstream of mitochondrial cytochrome c release. It is demonstrated that the block is due to deficiency of Apaf-1, a critical member of the apoptosome. Sensitivity to apoptosis was independent of caspase-9b or XIAP (inhibitors of apoptosis proteins) expression or levels of procaspase-9. Transfection of Apaf-1 conferred sensitivity to apoptosis in resistant cells. Apaf-1 deficiency may constitute a significant mode of resistance to apoptosis in human leukemia.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043683653382","@type":"Researcher","foaf:name":[{"@value":"Li Jia"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653376","@type":"Researcher","foaf:name":[{"@value":"Srinivasa M. Srinivasula"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653380","@type":"Researcher","foaf:name":[{"@value":"Feng-Ting Liu"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653378","@type":"Researcher","foaf:name":[{"@value":"Adrian C. Newland"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653381","@type":"Researcher","foaf:name":[{"@value":"Teresa Fernandes-Alnemri"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653377","@type":"Researcher","foaf:name":[{"@value":"Emad S. Alnemri"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043683653379","@type":"Researcher","foaf:name":[{"@value":"Stephen M. Kelsey"}],"jpcoar:affiliationName":[{"@value":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15280020"},{"@type":"PISSN","@value":"00064971"},{"@type":"NCID","@value":"AA00567156"}],"prism:publicationName":[{"@value":"Blood"}],"dc:publisher":[{"@value":"American Society of Hematology"}],"prism:publicationDate":"2001-07-15","prism:volume":"98","prism:number":"2","prism:startingPage":"414","prism:endingPage":"421"},"reviewed":"false","url":[{"@id":"http://ashpublications.org/blood/article-pdf/98/2/414/1675146/h8140100414.pdf"}],"createdAt":"2002-07-27","modifiedAt":"2020-02-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282677717214976","@type":"Article","resourceType":"紀要論文(departmental bulletin paper)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"神経膠芽腫における染色体12番長腕12q22-23のヘテロ接合性の喪失とApaf-1の不活化"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004237622668544","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Apaf1 plays a negative regulatory role in T cell responses by suppressing activation of antigen-stimulated T cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679326098944","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"抗癌剤投与による胃癌組織のアポトーシス誘導とミトコンドリア遺伝子の変動に関する免疫組織学的検討"},{"@language":"en","@value":"An Immunohistochemical Study of Apoptosis for Chemotherapeutic Effect on Gastric Cancer : Correlation with Mitochondrial Apoptotic Pathway"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679673200384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Role of Apaf-1 in Programmed Cell Death: From Worm to Tumor."},{"@language":"ja-Kana","@value":"Role of Apaf 1 in Programmed Cell Death From Worm to Tumor"}]},{"@id":"https://cir.nii.ac.jp/crid/1572261551094008192","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Humanized immunotoxins : A new generation of immunotoxins for targeted cancer therapy"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1182/blood.v98.2.414"},{"@type":"CIA","@value":"30022493445"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0195119_references_DOI_DkFWxybl5Prv1KIIUhTfZjP6wQt"},{"@type":"CROSSREF","@value":"10.1247/csf.28.3_references_DOI_DkFWxybl5Prv1KIIUhTfZjP6wQt"}]}