{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043888044288.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1476-5381.1993.tb13849.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1476-5381.1993.tb13849.x"}},{"identifier":{"@type":"URI","@value":"https://bpspubs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1476-5381.1993.tb13849.x"}}],"dc:title":[{"@value":"K channel activation by nucleotide diphosphates and its inhibition by glibenclamide in vascular smooth muscle cells"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p><jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Whole‐cell and inside‐out patch recordings were made from single smooth muscle cells that had been isolated enzymatically and mechanically from the rabbit portal vein.</jats:p></jats:list-item>\n<jats:list-item><jats:p>In whole‐cells the inclusion in the recording pipette solution of nucleotide diphosphates (NDPs), but not tri‐ or monophosphates, induced a K‐current that developed gradually over 5 to 15 min. Intracellular 1 m<jats:sc>m</jats:sc> guanosine 5′‐diphosphate (GDP) induced a slowly developing outward K‐current at − 37 mV that reached a maximum on average of 72 ± 4 pA (<jats:italic>n</jats:italic> = 40). Half maximal effect was estimated to occur with about 0.2 m<jats:sc>m</jats:sc> GDP. Except for ADP, other NDPs had comparable effects. At 0.1 m<jats:sc>m</jats:sc>, ADP was equivalent to GDP but at higher concentration ADP was less effective. ADP induced its maximum effect at 1 m<jats:sc>m</jats:sc> but had almost no effect at 10 m<jats:sc>m</jats:sc>.</jats:p></jats:list-item>\n<jats:list-item><jats:p>In 14% of inside‐out patches exposed to 1 m<jats:sc>m</jats:sc> GDP at the intracellular surface, characteristic K channel activity was observed which showed long (> 1 s) bursts of openings separated by longer closed periods. The current‐voltage relationship for the channel was linear in a 60 m<jats:sc>m</jats:sc>:130 m<jats:sc>m</jats:sc> K‐gradient and the unitary conductance was 24 pS.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Glibenclamide applied via the extracellular solution was found to be a potent inhibitor of GDP‐induced K‐current (<jats:italic>I</jats:italic><jats:sub>K(GDP)</jats:sub>) in the whole‐cell. The <jats:italic>K</jats:italic><jats:sub>d</jats:sub> was 25 n<jats:sc>m</jats:sc> and the inhibition was fully reversible on wash‐out.</jats:p></jats:list-item>\n<jats:list-item><jats:p>(<jats:italic>I</jats:italic><jats:sub>K(GDP)</jats:sub>) was not evoked if Mg ions were absent from the pipette solution. In contrast the omission of extracellular Mg ions had no effect on outward or inward (<jats:italic>I</jats:italic><jats:sub>K(GDP)</jats:sub>).</jats:p></jats:list-item>\n<jats:list-item><jats:p>Inclusion of 1 m<jats:sc>m</jats:sc> ATP in the recording pipette solution reduced (<jats:italic>I</jats:italic><jats:sub>K(GDP)</jats:sub>) and also attenuated its decline during long (25 min) recordings.</jats:p></jats:list-item>\n<jats:list-item><jats:p>When perforated‐patch whole‐cell recording was used, metabolic poisoning with cyanide and 2‐deoxy‐<jats:sc>d</jats:sc>‐glucose induced a glibenclamide‐sensitive K‐current. This current was not observed when conventional whole‐cell recording was used. Possible reasons for this difference are discussed.</jats:p></jats:list-item>\n<jats:list-item><jats:p>These K channels appear similar to ATP‐sensitive K channels but we refer to them as nucleotide diphosphate‐dependent K channels (K<jats:sub>NDP</jats:sub>) to emphasise what seems to be a primary role for nucleotide diphosphates in their regulation.</jats:p></jats:list-item>\n</jats:list></jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043888044288","@type":"Researcher","foaf:name":[{"@value":"D.J. Beech"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043888044291","@type":"Researcher","foaf:name":[{"@value":"H. Zhang"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043888044289","@type":"Researcher","foaf:name":[{"@value":"K. Nakao"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043888044290","@type":"Researcher","foaf:name":[{"@value":"T.B. Bolton"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00071188"},{"@type":"EISSN","@value":"14765381"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00071188"}],"prism:publicationName":[{"@value":"British Journal of Pharmacology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1993-10","prism:volume":"110","prism:number":"2","prism:startingPage":"573","prism:endingPage":"582"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1476-5381.1993.tb13849.x"},{"@id":"https://bpspubs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1476-5381.1993.tb13849.x"}],"createdAt":"2012-07-19","modifiedAt":"2023-10-24","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360025431125260032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Thomas (Tom) B. Bolton – a major force in smooth muscle research"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707094010752","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of ZD0947, a novel and potent ATP-sensitive K+ channel opener, on smooth muscle-type ATP-sensitive K+ channels"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710462029952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular analysis of<scp>ATP</scp>‐sensitive<scp><scp>K<sup>+</sup></scp></scp>channel subunits expressed in mouse vas deferens myocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166148673152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular analysis of ATP-sensitive K+ channel subunits expressed in mouse portal vein"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640981912576","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Diphosphate Regulation of Adenosine Triphosphate Sensitive Potassium Channel in Human Bladder Smooth Muscle Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205747359232","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mechanism of protective effects of magnesium on cardiac reperfusion injury"},{"@language":"ja","@value":"心臓の再灌流障害とマグネシウム"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679735070464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"血管平滑筋細胞のATP感受性K<sup>+</sup>チャネルと麻酔"},{"@language":"en","@value":"ATP-Sensitive Potassium Channels in Vascular Smooth Muscle Cells and Anesthesia"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680019641088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"ATP-Sensitive Potassium Channels: Structures, Functions, and Pathophysiology"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680153175296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of KRN4884, a Novel K+ Channel Opener, on Ionic Currents in Rabbit Femoral Arterial Myocytes"},{"@value":"Effects of KRN4884, a Novel K〔+〕 Channel Opener, on Ionic Currents in Rabbit Femoral Arterial Myocytes"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1476-5381.1993.tb13849.x"},{"@type":"CROSSREF","@value":"10.1113/jp287062_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.1111/bph.12437_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.2199/jjsca.25.126_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.14789/pjmj.52.28_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.1254/jphs.93.289_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.1016/j.juro.2011.03.153_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.2170/jjphysiol.48.397_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.1016/j.vph.2015.06.018_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"},{"@type":"CROSSREF","@value":"10.1016/j.ejphar.2016.09.038_references_DOI_Zqr360qHfFcuJViHSRGsMBxfs55"}]}