{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137043897017472.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/tpj.12566"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12566"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12566"}}],"dc:title":[{"@value":"Fatty acid synthesis and lipid metabolism in the obligate biotrophic fungus <i><scp>R</scp>hizophagus irregularis</i> during mycorrhization of <i><scp>L</scp>otus japonicus</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>Arbuscular mycorrhiza formation with fungi of the <jats:italic>Glomeromycota</jats:italic> represents a widespread symbiotic interaction of vascular plants. Different signaling events and metabolic adaptations are required for the close interaction between the two partners. Membrane lipid synthesis is a prerequisite for symbiosis, and membrane properties depend on lipid composition. Lipid profiling was performed by liquid chromatography mass spectrometry to study the role of triacylglycerol, diacylglycerol, phospholipids, galactolipids, sterols and sphingolipids during the colonization of <jats:italic><jats:styled-content style=\"fixed-case\">L</jats:styled-content>otus japonicus</jats:italic> roots with <jats:italic><jats:styled-content style=\"fixed-case\">R</jats:styled-content>hizophagus irregularis</jats:italic> (syn. <jats:italic><jats:styled-content style=\"fixed-case\">G</jats:styled-content>lomus intraradices</jats:italic>). Mycorrhization leads to an increased phosphate supply and suppresses the increase in galactolipids commonly observed in phosphate‐deprived plants. In addition to free sterols and sterol esters, <jats:italic>R. irregularis</jats:italic> contains sterol glucosides and acylated sterol glucosides. Glycosylated sphingolipids (glucosylceramide, dihexosylceramide) and inositolphosphorylceramide were detected in the fungus. Lyso‐phosphatidylcholine, a lipid previously implicated in mycorrhiza signaling, is present in low amounts in mock‐infected and mycorrhized roots. The composition of fungal phospholipids changes after mycorrhization because molecular species with palmitvaccenic (di‐16:1) or tetracosenoic (24:1) acyl groups decrease in intraradical mycelium. This adaptation of lipid metabolism during intraradical growth is likely a prerequisite for symbiosis, achieving functional compatibility between the fungal and the periarbuscular membrane. Data mining in genomic and transcript databases revealed the presence of genes encoding enzymes of lipid biosynthesis in <jats:italic>R. irregularis</jats:italic>. However, no gene encoding multidomain fatty acid <jats:italic>de novo</jats:italic> synthase was detected in the genome sequence of this obligate biotrophic fungus.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137043897017473","@type":"Researcher","foaf:name":[{"@value":"Vera Wewer"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular Physiology and Biotechnology of Plants (IMBIO) University of Bonn Karlrobert‐Kreiten‐Strasse 13 53115 Bonn Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043897017472","@type":"Researcher","foaf:name":[{"@value":"Mathias Brands"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular Physiology and Biotechnology of Plants (IMBIO) University of Bonn Karlrobert‐Kreiten‐Strasse 13 53115 Bonn Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137043897017474","@type":"Researcher","foaf:name":[{"@value":"Peter Dörmann"}],"jpcoar:affiliationName":[{"@value":"Institute of Molecular Physiology and Biotechnology of Plants (IMBIO) University of Bonn Karlrobert‐Kreiten‐Strasse 13 53115 Bonn Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09607412"},{"@type":"EISSN","@value":"1365313X"}],"prism:publicationName":[{"@value":"The Plant Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2014-07-02","prism:volume":"79","prism:number":"3","prism:startingPage":"398","prism:endingPage":"412"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12566"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12566"}],"createdAt":"2014-05-28","modifiedAt":"2023-10-02","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282813902870272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis"},{"@value":"RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in<i>Lotus japonicus</i>and<i>Rhizophagus irregularis</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234739485696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Lipid Droplets of Arbuscular Mycorrhizal Fungi Emerge in Concert with Arbuscule Collapse"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004236813492480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The genome of Rhizophagus clarus HR1 reveals a common genetic basis for auxotrophy among arbuscular mycorrhizal fungi"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142707546624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Myristate can be used as a carbon and energy source for the asymbiotic growth of arbuscular mycorrhizal fungi"}]},{"@id":"https://cir.nii.ac.jp/crid/1360022304969664768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Asymbiotic mass production of the arbuscular mycorrhizal fungus\n                  <i>Rhizophagus clarus</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360568469155020800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The relationship between thiamine and two symbioses: Root nodule symbiosis and arbuscular mycorrhiza"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521293248128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evidence of non-tandemly repeated rDNAs 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