{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044307862784.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1373/clinchem.2019.304048"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/clinchem/article-pdf/66/1/117/31700797/clinchem.2019.304048.pdf"}}],"dc:title":[{"@value":"Digital PCR—An Emerging Technology with Broad Applications in Microbiology"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND</jats:title>\n                  <jats:p>The PCR and its variant, quantitative PCR (qPCR), have revolutionized the practice of clinical microbiology. Continued advancements in PCR have led to a new derivative, digital PCR (dPCR), which promises to address certain limitations inherent to qPCR.</jats:p>\n               </jats:sec>\n               <jats:sec>\n                  <jats:title>CONTENT</jats:title>\n                  <jats:p>Here we highlight the important technical differences between qPCR and dPCR, and the potential advantages and disadvantages of each. We then review specific situations in which dPCR has been implemented in clinical microbiology and the results of such applications. Finally, we attempt to place dPCR in the context of other emerging technologies relevant to the clinical laboratory, including next-generation sequencing.</jats:p>\n               </jats:sec>\n               <jats:sec>\n                  <jats:title>SUMMARY</jats:title>\n                  <jats:p>dPCR offers certain clear advantages over traditional qPCR, but these are to some degree offset by limitations of the technology, at least as currently practiced. Laboratories considering implementation of dPCR should carefully weigh the potential advantages and disadvantages of this powerful technique for each specific application planned.</jats:p>\n               </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044307862785","@type":"Researcher","foaf:name":[{"@value":"Stephen J Salipante"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, University of Washington, Seattle, WA"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044307862784","@type":"Researcher","foaf:name":[{"@value":"Keith R Jerome"}],"jpcoar:affiliationName":[{"@value":"Department of Laboratory Medicine, University of Washington, Seattle, WA"},{"@value":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00099147"},{"@type":"EISSN","@value":"15308561"}],"prism:publicationName":[{"@value":"Clinical Chemistry"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2019-12-30","prism:volume":"66","prism:number":"1","prism:startingPage":"117","prism:endingPage":"123"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/clinchem/article-pdf/66/1/117/31700797/clinchem.2019.304048.pdf"}],"createdAt":"2019-11-08","modifiedAt":"2020-02-03","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360020701021479040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Current status of molecular diagnostic approaches using liquid biopsy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302864799538048","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Optimization of the 5-plex digital PCR workflow for simultaneous monitoring of SARS-CoV-2 and other pathogenic viruses in wastewater"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707162827520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Application of Coagulation and Foam Concentration Method to Quantify Waterborne Pathogens in River Water Samples"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865814731796736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Toward versatile digital bioanalysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869855121942656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater"}]},{"@id":"https://cir.nii.ac.jp/crid/1390869854627296128","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"マルチプレックスデジタルPCRを用いた下水中の薬剤耐性遺伝子の定量法の開発"},{"@language":"en","@value":"DEVELOPMENT OF A METHOD FOR QUANTIFICATION OF ANTIBIOTIC RESISTANCE GENES IN WASTEWATER USING MULTIPLEX DIGITAL PCR"}]},{"@id":"https://cir.nii.ac.jp/crid/1521136280081254400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Fast, sensitive, and reliable detection of waterborne pathogens by digital PCR after coagulation and foam concentration"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1373/clinchem.2019.304048"},{"@type":"CROSSREF","@value":"10.2208/jscejj.25-25017_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2020.02.004_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.1016/j.scitotenv.2023.169746_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.3390/w14223642_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.1063/5.0174727_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.1007/s00535-023-02024-4_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"},{"@type":"CROSSREF","@value":"10.1016/j.scitotenv.2025.178939_references_DOI_P8gP1eUcaENaII3AilE3TnYUSPd"}]}