{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044369956480.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1467-7652.2004.00091.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1467-7652.2004.00091.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1467-7652.2004.00091.x"}}],"dc:title":[{"@value":"Metabolic engineering of the mevalonate and non‐mevalonate isopentenyl diphosphate‐forming pathways for the production of health‐promoting isoprenoids in tomato"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>The genetic manipulation of both the mevalonic acid (MVA) and methylerythritol‐4‐phosphate (MEP) pathways, leading to the formation of isopentenyl diphosphate (IPP), has been achieved in tomato using 3‐hydroxymethylglutaryl CoA (<jats:italic>hmgr‐1</jats:italic>) and 1‐deoxy‐<jats:sc>d</jats:sc>‐xylulose‐5‐phosphate synthase (<jats:italic>dxs</jats:italic>) genes, respectively. Transgenic plants containing an additional <jats:italic>hmgr‐1</jats:italic> from <jats:italic>Arabidopsis thaliana</jats:italic>, under the control of the cauliflower mosaic virus (CaMV) 35S constitutive promoter, contained elevated phytosterols (up to 2.4‐fold), but IPP‐derived isoprenoids in the plastid were unaltered. Transgenic lines containing a bacterial <jats:italic>dxs</jats:italic> targeted to the plastid with the tomato <jats:italic>dxs</jats:italic> transit sequence resulted in an increased carotenoid content (1.6‐fold), which was inherited in the next generation. Phytoene and β‐carotene exhibited the greatest increases (2.4‐ and 2.2‐fold, respectively). Extra‐plastidic isoprenoids were unaffected in these lines. These data are discussed with respect to the regulation, compartmentalization and manipulation of isoprenoid biosynthetic pathways and their relevance to plant biotechnology.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044369956485","@type":"Researcher","foaf:name":[{"@value":"Eugenia M. A. Enfissi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044369956484","@type":"Researcher","foaf:name":[{"@value":"Paul D. Fraser"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298343643980800","@type":"Researcher","foaf:name":[{"@value":"Luisa‐Maria Lois"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044369956481","@type":"Researcher","foaf:name":[{"@value":"Albert Boronat"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044369956480","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Schuch"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044369956483","@type":"Researcher","foaf:name":[{"@value":"Peter M. Bramley"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"14677644"},{"@type":"EISSN","@value":"14677652"}],"prism:publicationName":[{"@value":"Plant Biotechnology Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2004-09-21","prism:volume":"3","prism:number":"1","prism:startingPage":"17","prism:endingPage":"27"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1467-7652.2004.00091.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1467-7652.2004.00091.x"}],"createdAt":"2005-01-12","modifiedAt":"2023-10-10","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050009062436053760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283694137013376","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Overexpression of 1-Deoxy-d-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204327164800","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Biochemical and molecular characterization of orange- and tangerine-colored rice calli"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205260580352","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Expression Profile of Genes Involved in Isoprenoid Biosynthesis in the Marine Diatom <i>Phaeodactylum tricornutum</i>"},{"@value":"Expression Profile of Genes Involved in Isoprenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303560704","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The genetic basis of foliar terpene yield: Implications for breeding and profitability of Australian essential oil crops"}]},{"@id":"https://cir.nii.ac.jp/crid/1390589221586263296","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Tomato lipocalins mediate ABA (abscisic acid)- and ethylene-dependent regulation of stress tolerance and fruit ripening"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1467-7652.2004.00091.x"},{"@type":"CROSSREF","@value":"10.1038/srep08753_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.1009a_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.15.0526a_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.25.1023b_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"},{"@type":"CROSSREF","@value":"10.2525/ecb.54.31_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"},{"@type":"CROSSREF","@value":"10.1263/jbb.105.518_references_DOI_7OxP9zU6uspAUEuIrdt1eFV0hot"}]}