{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044485079424.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.crvi.2009.01.002"}},{"identifier":{"@type":"URI","@value":"https://comptes-rendus.academie-sciences.fr/biologies/item/10.1016/j.crvi.2009.01.002.pdf"}}],"dc:title":[{"@value":"Purification and characterization of α-amylase from safflower (Carthamus tinctorius L.) germinating seeds"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                    α-Amylase (α-1-4 D-glucan glucanohydrolase EC 3.2.1.1) crude extract was obtained from safflower (\n                    <jats:italic>Carthamus tinctorius</jats:italic>\n                    L.) cotyledons excised from 5-day-old dark grown seedlings. The enzyme was purified by precipitating the crude extract with ammonium sulphate at 20–60% saturation, and then by subjecting this fraction to affinity chromatography on a β-cyclodextrin-Sepharose 6B column. The active fraction was dialysed and concentrated. An overall purification of about 131 folds with an activity yield of 81.25% was achieved. The molecular mass of purified enzyme determined by SDS-PAGE was 35 kD. When the purified α-amylase was subjected to gel electrophoresis followed by negative staining, only one band of active protein was detected. Its maximal activity was in the pH 6.0 and at a temperature of 55 °C. This enzyme was activated by Ca\n                    <jats:sup>2+</jats:sup>\n                    and inhibited by Fe\n                    <jats:sup>2+</jats:sup>\n                    .\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044485079425","@type":"Researcher","foaf:name":[{"@value":"Mosbah Ben Elarbi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044485079427","@type":"Researcher","foaf:name":[{"@value":"Halima Khemiri"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044485079424","@type":"Researcher","foaf:name":[{"@value":"Taoufik Jridi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044485079426","@type":"Researcher","foaf:name":[{"@value":"Jeannette Ben Hamida"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"16310691"},{"@type":"EISSN","@value":"17683238"}],"prism:publicationName":[{"@value":"Comptes Rendus. Biologies"}],"dc:publisher":[{"@value":"MathDoc/Centre Mersenne"}],"prism:publicationDate":"2009-02-27","prism:volume":"332","prism:number":"5","prism:startingPage":"426","prism:endingPage":"432"},"reviewed":"false","url":[{"@id":"https://comptes-rendus.academie-sciences.fr/biologies/item/10.1016/j.crvi.2009.01.002.pdf"}],"createdAt":"2009-02-28","modifiedAt":"2025-10-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1361131415976605824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Characterization of earthworm α-amylases for dietary supplement development and biomass utilization"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204326868480","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"α-Amylase from Mon Thong durian (<i>Durio zibethinus</i> Murr. cv. Mon Thong)-nucleotide sequence analysis, cloning and expression"},{"@value":"α-Amylase from Mon Thong durian (Durio zibethinus Murr. cv. Mon Thong)-nucleotide sequence analysis, cloning and expression"},{"@value":"&#x3b1;-Amylase from Mon Thong durian (<i>Durio zibethinus</i> Murr. cv. Mon Thong)-nucleotide sequence analysis, cloning and expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1390565134843562752","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Purification and characterization of thermostable α-amylase from germinating Sword bean (<i>Canavalia gladiata</i> (Jacq.) DC.) seeds"},{"@value":"Purification and characterization of thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.crvi.2009.01.002"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.1122a_references_DOI_PB9rZy3cOhXbOsOmE4JuhRASNoJ"},{"@type":"CROSSREF","@value":"10.1007/s11356-019-05133-x_references_DOI_PB9rZy3cOhXbOsOmE4JuhRASNoJ"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.19.1209b_references_DOI_PB9rZy3cOhXbOsOmE4JuhRASNoJ"}]}