{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044548132736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1600-0854.2007.00571.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0854.2007.00571.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0854.2007.00571.x"}}],"dc:title":[{"@value":"Rab27b Regulates Mast Cell Granule Dynamics and Secretion"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p><jats:bold>The Rab GTPase family regulates membrane domain organization and vesicular transport pathways. Recent studies indicate that one member of the family, Rab27a, regulates transport of lysosome‐related organelles in specialized cells, such as melanosomes and lytic granules. Very little is known about the related isoform, Rab27b. Here we used genetically modified mice to study the involvement of the Rab27 proteins in mast cells, which play key roles in allergic responses. Both Rab27a and Rab27b isoforms are expressed in bone marrow‐derived mast cells (BMMC) and localize to secretory granules. Nevertheless, secretory defects as measured by β‐hexosaminidase release <jats:italic>in vitro</jats:italic>and passive cutaneous anaphylaxis <jats:italic>in vivo</jats:italic>were found only in Rab27b and double Rab27 knockout (KO) mice. Immunofluorescence studies suggest that a subset of Rab27b and double Rab27‐deficient BMMCs exhibit mild clustering of granules. Quantitative analysis of live‐cell time‐lapse imaging revealed that BMMCs derived from double Rab27 KO mice showed almost 10‐fold increase in granules exhibiting fast movement (>1.5 μm/s), which could be disrupted by nocodazole. These results suggest that Rab27 proteins, particularly Rab27b, play a crucial role in mast cell degranulation and that their action regulates the transition from microtubule to actin‐based motility.</jats:bold></jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044548132738","@type":"Researcher","foaf:name":[{"@value":"Kouichi Mizuno"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132740","@type":"Researcher","foaf:name":[{"@value":"Tanya Tolmachova"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132741","@type":"Researcher","foaf:name":[{"@value":"Dmitry S. Ushakov"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132743","@type":"Researcher","foaf:name":[{"@value":"Maryse Romao"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132737","@type":"Researcher","foaf:name":[{"@value":"Magnus Åbrink"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132742","@type":"Researcher","foaf:name":[{"@value":"Michael A. Ferenczi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132739","@type":"Researcher","foaf:name":[{"@value":"Graça Raposo"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044548132736","@type":"Researcher","foaf:name":[{"@value":"Miguel C. Seabra"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13989219"},{"@type":"EISSN","@value":"16000854"}],"prism:publicationName":[{"@value":"Traffic"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-05-04","prism:volume":"8","prism:number":"7","prism:startingPage":"883","prism:endingPage":"892"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0854.2007.00571.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0854.2007.00571.x"}],"createdAt":"2007-05-09","modifiedAt":"2023-10-11","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232001078400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Orai-2 is localized on secretory granules and regulates antigen-evoked Ca2+ mobilization and exocytosis in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691837370240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"MicroRNA-155 Controls Exosome Synthesis and Promotes Gemcitabine Resistance in Pancreatic Ductal Adenocarcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283693338902016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab27 Effectors, Pleiotropic Regulators in Secretory Pathways"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706977147776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab27b regulates c-kit expression by controlling the secretion of stem cell factor"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617731089152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166754251648","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab27a and Rab27b control different steps of the exosome secretion pathway"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183487235584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A method for detailed analysis of the structure of mast cell secretory granules by negative contrast imaging"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118719985536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Large Rab GTPases: Novel Membrane Trafficking Regulators with a Calcium Sensor and Functional Domains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846642079973760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Large Scale Screening for Novel Rab Effectors Reveals Unexpected Broad Rab Binding Specificity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846644022431360","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab-genome analysis reveals novel insights in Weibel-Palade body exocytosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846645842633856","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Decoding the Regulation of Mast Cell Exocytosis by Networks of Rab GTPases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846645842987008","@type":"Article","resourceType":"学術雑誌論文(journal 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