{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044584444800.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/iej.12751"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fiej.12751"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/iej.12751"}}],"dc:title":[{"@value":"Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) <jats:styled-content style=\"fixed-case\">MTA</jats:styled-content> (Angelus, Londrina, <jats:styled-content style=\"fixed-case\">PR</jats:styled-content>, Brazil), Theracal <jats:styled-content style=\"fixed-case\">LC</jats:styled-content> (Bisco Inc., Schamburg, <jats:styled-content style=\"fixed-case\">IL</jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA</jats:styled-content>) and <jats:styled-content style=\"fixed-case\">IRM</jats:styled-content> (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (<jats:styled-content style=\"fixed-case\">SHED</jats:styled-content>s).</jats:p></jats:sec><jats:sec><jats:title>Methodology</jats:title><jats:p><jats:styled-content style=\"fixed-case\">SHED</jats:styled-content>s were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (<jats:styled-content style=\"fixed-case\">MTT</jats:styled-content>) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an <jats:italic>in vitro</jats:italic> scratch wound‐healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, <jats:styled-content style=\"fixed-case\">SHED</jats:styled-content>s were directly seeded onto the material surfaces and analysed by scanning electron microscopy (<jats:styled-content style=\"fixed-case\">SEM</jats:styled-content>). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; <jats:italic>P</jats:italic> < 0.01) and was also significantly higher than using <jats:styled-content style=\"fixed-case\">MTA</jats:styled-content> Angelus from 48 h of incubation (<jats:italic>P</jats:italic> < 0.01). However, Theracal <jats:styled-content style=\"fixed-case\">LC</jats:styled-content> and <jats:styled-content style=\"fixed-case\">IRM</jats:styled-content> were associated with low rates of cell viability (<jats:italic>P</jats:italic> < 0.001). Similar results were obtained in an apoptosis assay. In addition, <jats:styled-content style=\"fixed-case\">SHED</jats:styled-content>s maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. <jats:styled-content style=\"fixed-case\">SEM</jats:styled-content> studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and <jats:styled-content style=\"fixed-case\">MTA</jats:styled-content> Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7 days of culture (<jats:italic>P</jats:italic> < 0.01).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Biodentine exhibited better cytocompatibility and bioactivity than <jats:styled-content style=\"fixed-case\">MTA</jats:styled-content> Angelus, Theracal <jats:styled-content style=\"fixed-case\">LC</jats:styled-content> and <jats:styled-content style=\"fixed-case\">IRM</jats:styled-content>.</jats:p></jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380861290801157378","@type":"Researcher","foaf:name":[{"@value":"M. Collado‐González"}],"jpcoar:affiliationName":[{"@value":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia  Murcia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290801157377","@type":"Researcher","foaf:name":[{"@value":"D. García‐Bernal"}],"jpcoar:affiliationName":[{"@value":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia  Murcia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290801157376","@type":"Researcher","foaf:name":[{"@value":"R. E. Oñate‐Sánchez"}],"jpcoar:affiliationName":[{"@value":"School of Dentistry Faculty of Medicine University of Murcia  Murcia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290801157380","@type":"Researcher","foaf:name":[{"@value":"P. S. Ortolani‐Seltenerich"}],"jpcoar:affiliationName":[{"@value":"Department of Stomatology Universitat de Valencia Valencia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290801157381","@type":"Researcher","foaf:name":[{"@value":"T. Álvarez‐Muro"}],"jpcoar:affiliationName":[{"@value":"Dental Clinic Esdens  Alicante Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044584444805","@type":"Researcher","foaf:name":[{"@value":"A. Lozano"}],"jpcoar:affiliationName":[{"@value":"Department of Stomatology Universitat de Valencia Valencia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044584444809","@type":"Researcher","foaf:name":[{"@value":"L. Forner"}],"jpcoar:affiliationName":[{"@value":"Department of Stomatology Universitat de Valencia Valencia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044584444802","@type":"Researcher","foaf:name":[{"@value":"C. Llena"}],"jpcoar:affiliationName":[{"@value":"Department of Stomatology Universitat de Valencia Valencia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044584444803","@type":"Researcher","foaf:name":[{"@value":"J. M. Moraleda"}],"jpcoar:affiliationName":[{"@value":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia  Murcia Spain"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290801157379","@type":"Researcher","foaf:name":[{"@value":"F. J. Rodríguez‐Lozano"}],"jpcoar:affiliationName":[{"@value":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia  Murcia Spain"},{"@value":"School of Dentistry Faculty of Medicine University of Murcia  Murcia Spain"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01432885"},{"@type":"EISSN","@value":"13652591"}],"prism:publicationName":[{"@value":"International Endodontic Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2017-03-17","prism:volume":"50","prism:number":"S2","prism:startingPage":"e19"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fiej.12751"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/iej.12751"}],"createdAt":"2017-02-07","modifiedAt":"2023-09-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215887613696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Freshly-mixed and setting calcium-silicate cements stimulate human dental pulp cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390307746619244672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/iej.12751"},{"@type":"CROSSREF","@value":"10.4012/dmj.2025-102_references_DOI_Lfv1n7vtTOrg53BOWktz3wocu68"},{"@type":"CROSSREF","@value":"10.1016/j.dental.2018.02.005_references_DOI_Lfv1n7vtTOrg53BOWktz3wocu68"}]}