{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044720252032.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1107/s0907444901017371"}},{"identifier":{"@type":"URI","@value":"http://journals.iucr.org/d/issues/2002/01/00/ts0115/ts0115.pdf"}},{"identifier":{"@type":"PMID","@value":"11752795"}}],"dc:title":[{"@value":"Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K"}],"description":[{"notation":[{"@value":"Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044720252034","@type":"Researcher","foaf:name":[{"@value":"Takayuki Motoyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801184756224","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30227764"},{"@type":"NRID","@value":"1000030227764"},{"@type":"CINII_AUTHOR_ID","@value":"DA13553024"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DA13553024#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDA13553024"},{"@type":"NRID","@value":"9000002735690"},{"@type":"NRID","@value":"9000020310134"},{"@type":"NRID","@value":"9000020173414"},{"@type":"NRID","@value":"9000019955207"},{"@type":"NRID","@value":"9000020045841"},{"@type":"NRID","@value":"9000258297136"},{"@type":"NRID","@value":"9000258297137"},{"@type":"NRID","@value":"9000020254400"},{"@type":"NRID","@value":"9000020093699"},{"@type":"NRID","@value":"9000020080472"},{"@type":"NRID","@value":"9000241430552"},{"@type":"NRID","@value":"9000391926194"},{"@type":"NRID","@value":"9000397660061"},{"@type":"NRID","@value":"9000241430569"},{"@type":"NRID","@value":"9000413357345"},{"@type":"NRID","@value":"9000002744416"},{"@type":"NRID","@value":"9000020130258"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0164407"}],"foaf:name":[{"@value":"Masayoshi Nakasako"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044720252032","@type":"Researcher","foaf:name":[{"@value":"Isamu Yamaguchi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09074449"}],"prism:publicationName":[{"@value":"Acta Crystallographica Section D Biological Crystallography"}],"dc:publisher":[{"@value":"International Union of Crystallography (IUCr)"}],"prism:publicationDate":"2001-12-21","prism:volume":"58","prism:number":"1","prism:startingPage":"148","prism:endingPage":"150"},"reviewed":"false","dc:rights":["http://journals.iucr.org/services/copyrightpolicy.html","http://journals.iucr.org/services/copyrightpolicy.html#TDM"],"url":[{"@id":"http://journals.iucr.org/d/issues/2002/01/00/ts0115/ts0115.pdf"}],"createdAt":"2002-07-27","modifiedAt":"2025-01-11","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Conformation","dc:title":"Protein Conformation"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Crystallization","dc:title":"Crystallization"},{"@id":"https://cir.nii.ac.jp/all?q=Crystallography,%20X-Ray","dc:title":"Crystallography, X-Ray"},{"@id":"https://cir.nii.ac.jp/all?q=Hydro-Lyases","dc:title":"Hydro-Lyases"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050564288876566016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Solution Structure of IseA, an Inhibitor Protein of DL-Endopeptidases from Bacillus subtilis, Reveals a Novel Fold with a Characteristic Inhibitory Loop"},{"@value":"Solution structure of IseA, an inhibitor protein of DL-endopeptidase from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298755641588864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Prediction of hydrophilic and hydrophobic hydration structure of protein by neural network optimized using experimental data"}]},{"@id":"https://cir.nii.ac.jp/crid/1390024013997474432","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"深層学習によるタンパク質水和構造予測"},{"@language":"en","@value":"Prediction of Protein Hydration using Deep Learning"},{"@language":"ja-Kana","@value":"シンソウ ガクシュウ ニ ヨル タンパクシツ スイワ コウゾウ ヨソク"}]},{"@id":"https://cir.nii.ac.jp/crid/1390584870600098816","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Improvement in positional accuracy of neural-network predicted hydration sites of proteins by incorporating atomic details of water-protein interactions and site-searching algorithm"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1107/s0907444901017371"},{"@type":"OPENAIRE","@value":"doi_dedup___::6cc641b58afebab2f9a4a8e9fbcd1285"},{"@type":"CROSSREF","@value":"10.2142/biophysico.bppb-v22.0004_references_DOI_XyWSAYutbBPSTYgMhfqv9lOI3uZ"},{"@type":"CROSSREF","@value":"10.1038/s41598-023-29442-x_references_DOI_XyWSAYutbBPSTYgMhfqv9lOI3uZ"},{"@type":"CROSSREF","@value":"10.1074/jbc.m112.414763_references_DOI_XyWSAYutbBPSTYgMhfqv9lOI3uZ"},{"@type":"CROSSREF","@value":"10.5940/jcrsj.67.170_references_DOI_XyWSAYutbBPSTYgMhfqv9lOI3uZ"}]}