{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044720976768.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1126/science.277.5329.1078"}},{"identifier":{"@type":"URI","@value":"https://www.science.org/doi/pdf/10.1126/science.277.5329.1078"}},{"identifier":{"@type":"NAID","@value":"80009887807"}}],"dc:title":[{"@value":"Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044720976769","@type":"Researcher","foaf:name":[{"@value":"Robert Elghanian"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry, Northwestern University, Evanston, IL 60208, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044720976772","@type":"Researcher","foaf:name":[{"@value":"James J. Storhoff"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry, Northwestern University, Evanston, IL 60208, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044720976770","@type":"Researcher","foaf:name":[{"@value":"Robert C. Mucic"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry, Northwestern University, Evanston, IL 60208, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044720976771","@type":"Researcher","foaf:name":[{"@value":"Robert L. 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