{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044877834752.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/iai.71.9.5156-5162.2003"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/IAI.71.9.5156-5162.2003"}},{"identifier":{"@type":"NAID","@value":"30020831404"}}],"dc:title":[{"@value":"<i>Porphyromonas gingivalis</i>Infection during Pregnancy Increases Maternal Tumor Necrosis Factor Alpha, Suppresses Maternal Interleukin-10, and Enhances Fetal Growth Restriction and Resorption in Mice"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title><jats:p>Epidemiological studies have shown a potential association between maternal periodontitis and pregnancy complications. We used a pregnant murine model to study the effect of infection with the periodontal pathogen<jats:italic>Porphyromonas gingivalis</jats:italic>on pregnancy outcomes. Female BALB/c mice were inoculated with heat-killed<jats:italic>P. gingivalis</jats:italic>(10<jats:sup>9</jats:sup>CFU) in a subcutaneous chamber and mated 2 weeks later. At gestation day (GD) 7.5, mice were challenged with live<jats:italic>P. gingivalis</jats:italic>(10<jats:sup>7</jats:sup>CFU) (<jats:italic>n</jats:italic>= 20) or broth (control;<jats:italic>n</jats:italic>= 8) and sacrificed at GD 16.5. Fetal growth restriction (FGR, <0.46 g) was defined as fetuses with weights 2 standard deviations (SD) smaller than controls (0.56 ± 0.05 g [mean ± SD]). Among the 20 challenged mice, 8 had both normal-weight (0.51 ± 0.11 g) and FGR (0.34 ± 0.1 g) fetuses within the same litter. All other challenged dams had normal-weight fetuses (0.57 ± 0.04 g). Maternal liver, uterus, and spleen samples were examined for<jats:italic>P. gingivalis</jats:italic>DNA using a PCR technique. Of the eight challenged mice with FGR fetuses, three had PCR signals for<jats:italic>P. gingivalis</jats:italic>in liver and uterus, but not in the spleen. Liver, uterus, and spleen were negative for<jats:italic>P. gingivalis</jats:italic>DNA among all other challenged and control mice. In serum of dams with FGR fetuses, tumor necrosis factor alpha levels were elevated significantly, while interluekin-10 levels were significantly reduced compared to levels in dams with normal fetuses.<jats:italic>P. gingivalis</jats:italic>-specific serum immunoglobulin G levels were significantly elevated in dams with FGR fetuses compared to dams without any FGR fetuses. These data demonstrate that<jats:italic>P. gingivalis</jats:italic>-induced murine FGR is associated with systemic dissemination of the organism and activated maternal immune and inflammatory responses.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044877834755","@type":"Researcher","foaf:name":[{"@value":"Dongming Lin"}],"jpcoar:affiliationName":[{"@value":"Center for Oral and Systemic Diseases"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044877834757","@type":"Researcher","foaf:name":[{"@value":"Mary Alice Smith"}],"jpcoar:affiliationName":[{"@value":"Center for Oral and Systemic Diseases"},{"@value":"Environmental Health Science Department, University of Georgia, Athens, Georgia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044877834753","@type":"Researcher","foaf:name":[{"@value":"Catherine Champagne"}],"jpcoar:affiliationName":[{"@value":"Center for Oral and Systemic Diseases"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044877834756","@type":"Researcher","foaf:name":[{"@value":"John Elter"}],"jpcoar:affiliationName":[{"@value":"Comprehensive Center for Inflammatory Disorders, Department of Dental Ecology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044877834754","@type":"Researcher","foaf:name":[{"@value":"James Beck"}],"jpcoar:affiliationName":[{"@value":"Center for Oral and Systemic Diseases"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044877834752","@type":"Researcher","foaf:name":[{"@value":"Steven Offenbacher"}],"jpcoar:affiliationName":[{"@value":"Center for Oral and Systemic Diseases"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00199567"},{"@type":"EISSN","@value":"10985522"},{"@type":"NCID","@value":"AA00673732"}],"prism:publicationName":[{"@value":"Infection and Immunity"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2003-09","prism:volume":"71","prism:number":"9","prism:startingPage":"5156","prism:endingPage":"5162"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/IAI.71.9.5156-5162.2003"}],"createdAt":"2003-08-21","modifiedAt":"2024-01-10","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282812687453056","@type":"Article","resourceType":"紀要論文(departmental bulletin paper)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"歯周病原細菌である黒色集落形成嫌気性桿菌研究５０年の進歩"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002217158797440","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of<i>Porphyromonas gingivalis</i>infection in the placenta and umbilical cord in pregnant mice with low birth weight"}]},{"@id":"https://cir.nii.ac.jp/crid/1360025431125763840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"<i>P. gingivalis</i>–Infected Macrophage Extracellular Vesicles Cause Adverse Pregnancy Outcomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707424536960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182070793728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibition of gingipains prevents Porphyromonas gingivalis-induced preterm birth and fetal death in pregnant mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185927726592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis Require Jun N-Terminal Protein Kinase- and p53-Mediated p38 Activation in Human Trophoblasts"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660716804992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Periodontal diseases and adverse pregnancy outcomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1391694356247064832","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Lacking Interleukin-10 Regulates the Inflammasome-driven Alveolar Bone Loss"}]},{"@id":"https://cir.nii.ac.jp/crid/1572261551103371648","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Localization of P. gingivalis in Preterm Delivery Placenta"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/iai.71.9.5156-5162.2003"},{"@type":"CIA","@value":"30020831404"},{"@type":"CROSSREF","@value":"10.1080/00016357.2018.1426876_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.1177/00220345241285132_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.1128/iai.00923-17_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.1016/j.placenta.2014.10.015_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.1111/jog.13782_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.1016/j.ejphar.2018.01.028_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"},{"@type":"CROSSREF","@value":"10.5466/ijoms.19.184_references_DOI_AVW8YlFScNC3UnZ9g1ngOyFkHJH"}]}