{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137044999675648.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1074/jbc.m503645200"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820791921?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820791921?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1074/jbc.M503645200"}},{"identifier":{"@type":"PMID","@value":"16027159"}},{"identifier":{"@type":"NAID","@value":"80017502606"}}],"dc:title":[{"@value":"Chondroitin Sulfate Characterized by the E-disaccharide Unit Is a Potent Inhibitor of Herpes Simplex Virus Infectivity and Provides the Virus Binding Sites on gro2C Cells"}],"description":[{"notation":[{"@value":"Although cell surface chondroitin sulfate (CS) is regarded as an auxiliary receptor for binding of herpes simplex virus to cells, and purified CS chain types A, B, and C are known to interfere poorly or not at all with the virus infection of cells, we have found that CS type E (CS-E), derived from squid cartilage, exhibited potent antiviral activity. The IC(50) values ranged from 0.06 to 0.2 mug/ml and substantially exceeded the antiviral potency of heparin, the known inhibitor of virus binding to cells. Furthermore, in mutant gro2C cells that express CS but not heparan sulfate, CS-E showed unusually high anti-herpes virus activity with IC(50) values of1 ng/ml. Enzymatic degradation of CS-E with chondroitinase ABC abolished its antiviral activity. CS-E inhibited the binding to cells of the purified virus attachment protein gC. A direct interaction of gC with immobilized CS-E and inhibition of this binding by CS-E oligosaccharide fragments greater than octasaccharide were demonstrated. Likewise, the gro2C-specific CS chains interfered with the binding of viral gC to these cells and were found to contain a considerable proportion (13%) of the E-disaccharide unit, suggesting that this unit is an essential component of the CS receptor for herpes simplex virus on gro2C cells and that the antiviral activity of CS-E was due to interference with the binding of viral gC to a CS-E-like receptor on the cell surface. Knowledge of the determinants of antiviral properties of CS-E will help in the development of inhibitors of herpes simplex virus infections in humans."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137044999675654","@type":"Researcher","foaf:name":[{"@value":"Kicki Bergefall"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675649","@type":"Researcher","foaf:name":[{"@value":"Edward Trybala"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675653","@type":"Researcher","foaf:name":[{"@value":"Maria Johansson"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675652","@type":"Researcher","foaf:name":[{"@value":"Toru Uyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675655","@type":"Researcher","foaf:name":[{"@value":"Satomi Naito"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675648","@type":"Researcher","foaf:name":[{"@value":"Shuhei Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675651","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Kitagawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675650","@type":"Researcher","foaf:name":[{"@value":"Kazuyuki Sugahara"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137044999675656","@type":"Researcher","foaf:name":[{"@value":"Tomas Bergström"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219258"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00219258"}],"prism:publicationName":[{"@value":"Journal of Biological Chemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2005-09","prism:volume":"280","prism:number":"37","prism:startingPage":"32193","prism:endingPage":"32199"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0021925820791921?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0021925820791921?httpAccept=text/plain"},{"@id":"https://syndication.highwire.org/content/doi/10.1074/jbc.M503645200"}],"createdAt":"2005-07-19","modifiedAt":"2022-01-05","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Binding%20Sites","dc:title":"Binding Sites"},{"@id":"https://cir.nii.ac.jp/all?q=Dose-Response%20Relationship,%20Drug","dc:title":"Dose-Response Relationship, Drug"},{"@id":"https://cir.nii.ac.jp/all?q=Chondroitin%20Sulfates","dc:title":"Chondroitin Sulfates"},{"@id":"https://cir.nii.ac.jp/all?q=Decapodiformes","dc:title":"Decapodiformes"},{"@id":"https://cir.nii.ac.jp/all?q=Oligosaccharides","dc:title":"Oligosaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Enzyme-Linked%20Immunosorbent%20Assay","dc:title":"Enzyme-Linked Immunosorbent Assay"},{"@id":"https://cir.nii.ac.jp/all?q=Chondroitin%20ABC%20Lyase","dc:title":"Chondroitin ABC Lyase"},{"@id":"https://cir.nii.ac.jp/all?q=Disaccharides","dc:title":"Disaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Ligands","dc:title":"Ligands"},{"@id":"https://cir.nii.ac.jp/all?q=Antiviral%20Agents","dc:title":"Antiviral Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Inhibitory%20Concentration%2050","dc:title":"Inhibitory Concentration 50"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Cartilage","dc:title":"Cartilage"},{"@id":"https://cir.nii.ac.jp/all?q=Chlorocebus%20aethiops","dc:title":"Chlorocebus aethiops"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Simplexvirus","dc:title":"Simplexvirus"},{"@id":"https://cir.nii.ac.jp/all?q=Heparitin%20Sulfate","dc:title":"Heparitin Sulfate"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Binding","dc:title":"Protein Binding"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050022853110732672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1050025031478175872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Expression of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis"},{"@value":"Expression of<i>N</i>-Acetylgalactosamine 4-Sulfate 6-<i>O</i>-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis"},{"@value":"Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chodroitin sulfate synthesis is responsible for pulmonary metastasis"}]},{"@id":"https://cir.nii.ac.jp/crid/1050025031478176896","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium"}]},{"@id":"https://cir.nii.ac.jp/crid/1050025031478178304","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A Novel Eliminase from a Marine Bacterium That Degrades Hyaluronan 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