{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137045201184896.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aac.00375-06"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AAC.00375-06"}}],"dc:title":[{"@value":"Action of Disinfectant Quaternary Ammonium Compounds against<i>Staphylococcus aureus</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title><jats:p>Mode-of-action studies concluded that alkyldimethylbenzylammonium chloride (ADBAC) (a blend of C<jats:sub>12</jats:sub>, C<jats:sub>14</jats:sub>and C<jats:sub>16</jats:sub>alkyl homologues) and didecyldimethylammonium chloride (DDAC) are both membrane-active agents, possessing subtly different modes of action reflecting early cell interactions against<jats:italic>Staphylococcus aureus</jats:italic>. ADBAC and DDAC exhibited similar MIC behaviors from 0.4 ppm to 1.8 ppm over an inoculum range of 1 × 10<jats:sup>5</jats:sup>to 1 × 10<jats:sup>9</jats:sup>CFU/ml at 35°C. For ADBAC and DDAC, an increased rapidity of killing against<jats:italic>S. aureus</jats:italic>(final concentration, 2 × 10<jats:sup>9</jats:sup>CFU/ml) was observed at 35°C compared to 25°C. Concentration exponents (η) for killing were <2.5 for both agents, and temperature influenced the η value. Examination of leakage and kill data suggested that a single leakage marker was not indicative of cell death. ADBAC and DDAC possessed Langmuir (L4) and high-affinity (H3/4) uptake isotherms, respectively. ADBAC molecules formed a single monolayer of coverage of cells at the end of primary uptake, and DDAC formed a double monolayer. Rapid cell leakage occurred at bactericidal concentrations, with total depletion of the intracellular potassium and 260-nm-absorbing pools released in this strict order. Autolysis was observed for ADBAC and DDAC at concentrations of 9 μg/ml (0.0278 mM and 0.0276 mM, respectively) and above, together with the depletion of approximately 30% of the internal potassium pool. Autolysis contributed to ADBAC and DDAC lethality, although high biocide concentrations may have inhibited autolytic enzyme activity.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137045201184898","@type":"Researcher","foaf:name":[{"@value":"Christopher J. Ioannou"}],"jpcoar:affiliationName":[{"@value":"Quest International, Ashford, Kent TN24 0LT, United Kingdom"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045201184897","@type":"Researcher","foaf:name":[{"@value":"Geoff W. Hanlon"}],"jpcoar:affiliationName":[{"@value":"School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, United Kingdom"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045201184896","@type":"Researcher","foaf:name":[{"@value":"Stephen P. Denyer"}],"jpcoar:affiliationName":[{"@value":"Welsh School of Pharmacy, Cardiff University, Cardiff CF1 3XF, United Kingdom"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00664804"},{"@type":"EISSN","@value":"10986596"}],"prism:publicationName":[{"@value":"Antimicrobial Agents and Chemotherapy"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2007-01","prism:volume":"51","prism:number":"1","prism:startingPage":"296","prism:endingPage":"306"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AAC.00375-06"}],"createdAt":"2006-12-20","modifiedAt":"2025-01-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360290617865549952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Specific localisation of ions in bacterial membranes unravels physical mechanism of effective bacteria killing by sanitiser"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865814737896448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effectiveness of perioperative oral hygiene management using a cetylpyridinium chloride‐, dipotassium glycyrrhizinate, and tranexamic acid‐based mouthwash as an adjunct to mechanical oral hygiene in patients with maxillomandibular fixation: A randomized controlled clinical trial"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865816783784064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Thermodynamics and Viscoelastic Property of Interface Unravel Combined Functions of Cationic Surfactant and Aromatic Alcohol against Gram-Negative Bacteria"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204414164224","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Pulmonary Fibrosis in Response to Environmental Cues and Molecular Targets Involved in Its Pathogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679068399744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Factors Affecting the Phase Behavior and Antimicrobial Activity of Carvacrol Microemulsions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679323515904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Stress Tolerance of <i>Methylobacterium</i> Biofilms in Bathrooms"},{"@value":"Stress Tolerance of Methylobacterium Biofilms in Bathrooms"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679441851008","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Synergistic Bactericidal Effects of a Sublethal Concentration of Didecyldimethylammonium chloride  (DDAC)  and Low Concentrations of Nonionic Surfactants against <I>Staphylococcus aureus</I>"},{"@value":"Synergistic Bactericidal Effects of a Sublethal Concentration of Didecyldimethylammonium chloride (DDAC) and Low Concentrations of Nonionic Surfactants against Staphylococcus aureus"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679882267520","@type":"Article","resourceType":"letter","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Acute pulmonary toxicity and inflammation induced by combined exposure to didecyldimethylammonium chloride and ethylene glycol in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680266603008","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"When is a Vase Solution Biocide not, or not only, Antimicrobial?"},{"@language":"ja","@value":"生け水に添加する殺菌剤の多面的効能"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282763093537920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Susceptibility of bacteria isolated from dental unit waterlines to disinfecting chemical agents"}]},{"@id":"https://cir.nii.ac.jp/crid/1390564238080349312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Susceptibility of Oral Streptococci to Chlorhexidine and Cetylpyridinium Chloride"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/aac.00375-06"},{"@type":"CROSSREF","@value":"10.5650/jos.ess14194_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.1038/s41598-020-69064-1_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.2323/jgam.2018.02.001_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.1264/jsme2.me12146_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.4265/bio.24.13_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.1293/tox.24.9_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.4265/bio.17.175_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.1002/cre2.814_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.1021/acs.langmuir.3c00862_references_DOI_Ne5GA2AuAiou0CZSyJkTBMIXoWD"},{"@type":"CROSSREF","@value":"10.2503/jjshs1.77.211_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"},{"@type":"CROSSREF","@value":"10.2131/jts.41.17_references_DOI_8zGolqPs6qLUT21Sbl9b5xs5LTc"}]}