{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137045294163200.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1399-3054.1988.tb02044.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1988.tb02044.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1988.tb02044.x"}},{"identifier":{"@type":"NAID","@value":"30013430252"}}],"dc:title":[{"@value":"Homoglutathione: isolation, quantification and occurrence in legumes"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Homoglutathione (hGSH: γ‐glutamyl‐eysteinyl‐β‐alanine) was purified from seeds of <jats:italic>Phaseolus coccineus</jats:italic> L. cv. Preisgewinner, using anion‐exchange chromatography and Cu<jats:sub>2</jats:sub>O precipitation. Quantitative and specific determination of this thiol is possible by high‐performance liquid chromatography (HPLC) after monobromobimane derivatization. The enzymatic recycling assay based on yeast glutathione reductase (EC 1.6.4.2) can also be applied, but only to samples containing either hGSH or glutathione (GSH), since enzyme reaction with hGSH is 2.7 times faster than with GSH. Using the very sensitive HPLC method, the thiol content of leaves, roots and seeds of several legumes was investigated. Although GSH and hGSH were found in all plants analysed, the GSH/hGSH ratio varied greatly within the different tribes as well as within the different organs of plants of one species. In seeds and leaves of Vicieae, only traces of hGSH were found beside the main thiol GSH, whereas in roots the hGSH content exceeded the GSH content. The Trifolieae contained both tripeptides and in the tribe Phaseoleae, hGSH predominated by far.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1580009751478249984","@type":"Researcher","foaf:name":[{"@value":"S. Klapheck"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00319317"},{"@type":"EISSN","@value":"13993054"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00319317"},{"@type":"NCID","@value":"AA00774354"}],"prism:publicationName":[{"@value":"Physiologia Plantarum"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1988-12","prism:volume":"74","prism:number":"4","prism:startingPage":"727","prism:endingPage":"732"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1988.tb02044.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1988.tb02044.x"}],"createdAt":"2006-05-01","modifiedAt":"2023-09-27","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001205087416704","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"植物における活性酸素障害とその防御機構"},{"@language":"en","@value":"Oxidative Stresses and Defense Systems Against Them in Plants"},{"@value":"植物における活性酸素傷害とその防御機構"},{"@language":"ja-Kana","@value":"ショクブツ ニ オケル カッセイ サンソ ショウガイ ト ソノ ボウギョ キコウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206558615936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"イオウ欠乏に応答したダイズの葉の平行光屈性"},{"@language":"en","@value":"Paraheliotropism of Soybean Leaves in Response to Sulfur Deficiency"},{"@language":"ja-Kana","@value":"イオウ ケツボウ ニ オウトウ シタ ダイズ ノ ハ ノ ヘイコウ ヒカリ クッセイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679306014336","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Homoglutathione Synthesis in Transgenic Tobacco Plants Expressing Soybean Homoglutathione Synthetase"}]},{"@id":"https://cir.nii.ac.jp/crid/2050025942085740800","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"The Function of Homoglutathione and Hydroxymethylglutathione for the Scavenging of Hydrogen Peroxied"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1399-3054.1988.tb02044.x"},{"@type":"CIA","@value":"30013430252"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.21.79_references_DOI_YX3iLNItqGNZuwyviqVwAuWNv3b"}]}