{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137045447202944.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/em.2850220309"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fem.2850220309"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/em.2850220309"}},{"identifier":{"@type":"NAID","@value":"30025821240"}}],"dc:title":[{"@value":"Antimutagenic potency of chlorophyllin in the salmonella assay and its correlation with binding constants of mutagen‐inhibitor complexes"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Chlorophyllin (CHL) is a water‐soluble salt of chlorophyll that exhibits antimutagenic activity in short‐term genotoxicity assays and inhibits carcinogen‐DNA binding in vivo. The antimutagenic potency of CHL was studied against several structurally related heterocyclic amines using the Salmonella assay. The mutagens included 2‐amino‐3‐methylimidazo[4,5,‐<jats:italic>f</jats:italic>]‐quinoline (IQ) and seven related IQ‐type compounds, and 3‐amino‐1‐methyl‐5<jats:italic>H</jats:italic>‐pyrido[4,3‐<jats:italic>b</jats:italic>]indole (Trp‐P‐2) and three additional non‐IQ‐type compounds. No relationship was observed between mutagenic potency (revertants/ng mutagen) and antimutagenic potency when expressed in terms of the CHL dose/plateinhibiting mutagenicity by 50 percent (l<jats:sub>50</jats:sub>). However, a correlation was observed between mutagenic potency and the mole ratio of CHL to mutagen giving 50% inhibition (MR<jats:sub>50</jats:sub>), with most mutagens requiring several hundredfold to several thousandfold molar excess of CHL for inhibition. In spectrophotometric studies, CHL formed noncovalent molecular complexes with the heterocyclic amines, with binding constants in the range 3–13 x 10<jats:sup>3</jats:sup> M<jats:sup>−1</jats:sup>. Binding constants were inversely correlated with l<jats:sub>50</jats:sub> and MR<jats:sub>50</jats:sub> values, i.e., with increasing strength of complex formation less CHL/plate and a lower mole ratio of CHL to mutagen was required to inhibit mutagenicity. The results support an inhibitory mechanism in which chlorophylls operate as “interceptor molecules,” interacting with carcinogens and mutagens directly and limiting their bioavailability. © 1993 Wiley‐Liss, Inc.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137045447202945","@type":"Researcher","foaf:name":[{"@value":"Roderick Dashwood"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045447202944","@type":"Researcher","foaf:name":[{"@value":"Dexin Guo"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08936692"},{"@type":"EISSN","@value":"10982280"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/03865495"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/08936692"}],"prism:publicationName":[{"@value":"Environmental and Molecular Mutagenesis"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1993-01","prism:volume":"22","prism:number":"3","prism:startingPage":"164","prism:endingPage":"171"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fem.2850220309"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/em.2850220309"}],"createdAt":"2007-02-21","modifiedAt":"2023-10-25","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001204104995584","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mechanism of Action of Chlorophyllin against Mitomycin-C Mutagenicity in Allium cepa"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204455438080","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Desmutagenic Effect of Pheophytin b against Trp-P-2 in the Salmonella Assay."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204625016320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"No Effects of Chlorophyllin on IQ (2-Amino-3-methylimidazo[4,5-f]-quinoline)-genotoxicity and -DNA Adduct Formation in Drosophila"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204627700736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Preventive Effects of Chlorophyllin Fixed on Chitosan towards DNA Adduct Formation of 3-Amino-1-methyl-5<i>H</i>-pyrido[4,3-<i>b</i>]indole in CDF<sub>1</sub> Mice"},{"@value":"Preventive Effects of Chlorophyllin Fixed on Chitosan towards DNA Adduct Formation of 3-Amino-1-methyl-5H-pyrido[4,3-b]indole in CDF1 Mice"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/em.2850220309"},{"@type":"CIA","@value":"30025821240"},{"@type":"CROSSREF","@value":"10.1508/cytologia.69.459_references_DOI_FNaesdugyiApNA0U4KhNXL1AT7X"},{"@type":"CROSSREF","@value":"10.3136/fsti9596t9798.4.66_references_DOI_FNaesdugyiApNA0U4KhNXL1AT7X"},{"@type":"CROSSREF","@value":"10.1248/bpb.25.520_references_DOI_FNaesdugyiApNA0U4KhNXL1AT7X"},{"@type":"CROSSREF","@value":"10.1248/bpb.27.242_references_DOI_FNaesdugyiApNA0U4KhNXL1AT7X"}]}