{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137045504803840.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1083/jcb.148.4.703"}},{"identifier":{"@type":"URI","@value":"https://rupress.org/jcb/article-pdf/148/4/703/1855941/9912111.pdf"}}],"dc:title":[{"@value":"Debcl, a Proapoptotic Bcl-2 Homologue, Is a Component of the <i>Drosophila melanogaster</i> Cell Death Machinery"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Bcl-2 family of proteins are key regulators of apoptosis. Both proapoptotic and antiapoptotic members of this family are found in mammalian cells, but no such proteins have been described in insects. Here, we report the identification and characterization of Debcl, the first Bcl-2 homologue in Drosophila melanogaster. Structurally, Debcl is similar to Bax-like proapoptotic Bcl-2 family members. Ectopic expression of Debcl in cultured cells and in transgenic flies causes apoptosis, which is inhibited by coexpression of the baculovirus caspase inhibitor P35, indicating that Debcl is a proapoptotic protein that functions in a caspase-dependent manner. debcl expression correlates with developmental cell death in specific Drosophila tissues. We also show that debcl genetically interacts with diap1 and dark, and that debcl-mediated apoptosis is not affected by gene dosage of rpr, hid, and grim. Biochemically, Debcl can interact with several mammalian and viral prosurvival Bcl-2 family members, but not with the proapoptotic members, suggesting that it may regulate apoptosis by antagonizing prosurvival Bcl-2 proteins. RNA interference studies indicate that Debcl is required for developmental apoptosis in Drosophila embryos. These results suggest that the main components of the mammalian apoptosis machinery are conserved in insects.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137045504803843","@type":"Researcher","foaf:name":[{"@value":"Paul A. Colussi"}],"jpcoar:affiliationName":[{"@value":"aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803840","@type":"Researcher","foaf:name":[{"@value":"Leonie M. Quinn"}],"jpcoar:affiliationName":[{"@value":"bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803842","@type":"Researcher","foaf:name":[{"@value":"David C.S. Huang"}],"jpcoar:affiliationName":[{"@value":"cThe Walter and Eliza Hall Institute of Medical Research, The Royal Melbourne Hospital, Melbourne, Vic 3050, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803846","@type":"Researcher","foaf:name":[{"@value":"Michelle Coombe"}],"jpcoar:affiliationName":[{"@value":"bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803845","@type":"Researcher","foaf:name":[{"@value":"Stuart H. Read"}],"jpcoar:affiliationName":[{"@value":"aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803844","@type":"Researcher","foaf:name":[{"@value":"Helena Richardson"}],"jpcoar:affiliationName":[{"@value":"bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045504803841","@type":"Researcher","foaf:name":[{"@value":"Sharad Kumar"}],"jpcoar:affiliationName":[{"@value":"aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219525"},{"@type":"EISSN","@value":"15408140"}],"prism:publicationName":[{"@value":"The Journal of Cell Biology"}],"dc:publisher":[{"@value":"Rockefeller University Press"}],"prism:publicationDate":"2000-02-21","prism:volume":"148","prism:number":"4","prism:startingPage":"703","prism:endingPage":"714"},"reviewed":"false","url":[{"@id":"https://rupress.org/jcb/article-pdf/148/4/703/1855941/9912111.pdf"}],"createdAt":"2002-07-26","modifiedAt":"2023-07-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232167757312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710491978496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tumor suppressor gene <i><scp>OSCP</scp>1</i>/<i><scp>NOR</scp>1</i> regulates apoptosis, proliferation, differentiation, and <scp>ROS</scp> generation during eye development of <i>Drosophila melanogaster</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285712569713664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181948639488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"NF-Y in invertebrates"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580230636174464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evidence for existence of an apoptosis‐inducing\n                    <scp>BH3</scp>\n                    ‐only protein,\n                    <i>sayonara</i>\n                    , in\n                    <i>Drosophila</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869856043287168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Exploring caspase-dependent non-lethal cellular processes using Drosophila"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206409640064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular Genetic Control of Caspases and JNK-mediated Neural Cell Death."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679673200384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Role of Apaf-1 in Programmed Cell Death: From Worm to Tumor."},{"@language":"ja-Kana","@value":"Role of Apaf 1 in Programmed Cell Death From Worm to Tumor"}]},{"@id":"https://cir.nii.ac.jp/crid/1524232505125763840","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Differential gene expression in the hypopharyngeal glands of worker honeybees (Apis mellifera L.) associated with an age-dependent role change"},{"@language":"ja-Kana","@value":"Differential gene expression in the hypopharyngeal glands of worker honeybees Apis mellifera L associated with an age dependent role 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