{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137045668967808.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1104/pp.104.050567"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/plphys/article-pdf/136/4/4228/37813941/plphys_v136_4_4228.pdf"}},{"identifier":{"@type":"NAID","@value":"80017219890"}}],"dc:title":[{"@value":"Rice Contains Two Disparate <i>ent</i>-Copalyl Diphosphate Synthases with Distinct Metabolic Functions"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>Rice (Oryza sativa) produces ent-copalyl diphosphate for both gibberellin (GA) phytohormone and defensive phytoalexin biosynthesis, raising the question of how this initial biosynthetic step is carried out for these distinct metabolic processes. Here, a functional genomics approach has been utilized to identify two disparate ent-copalyl diphosphate synthases from rice (OsCPS1ent and OsCPS2ent). Notably, it was very recently demonstrated that only one of these (OsCPS1ent) normally operates in GA biosynthesis as mutations in this gene result in severely impaired growth. Evidence is presented here strongly indicating that the other (OsCPS2ent) is involved in related secondary metabolism producing defensive phytochemicals. In particular, under appropriate conditions, OsCPS2ent mRNA is specifically induced in leaves prior to production of the corresponding phytoalexins. Thus, transcriptional control of OsCPS2ent seems to be an important means of regulating defensive phytochemical biosynthesis. Finally, OsCPS1ent is significantly more similar to the likewise GA-specific gene An1/ZmCPS1ent in maize (Zea mays) than its class II terpene synthase paralogs involved in rice secondary metabolism. Hence, we speculate that this cross-species conservation by biosynthetic process reflects derivation of related secondary metabolism from the GA primary biosynthetic pathway prior to the early divergence between the separate lineages within the cereal/grass family (Poaceae) resulting in modern rice and maize.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137045668967810","@type":"Researcher","foaf:name":[{"@value":"Sladjana Prisic"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045668967811","@type":"Researcher","foaf:name":[{"@value":"Meimei Xu"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045668967808","@type":"Researcher","foaf:name":[{"@value":"P. Ross Wilderman"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137045668967809","@type":"Researcher","foaf:name":[{"@value":"Reuben J. Peters"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15322548"},{"@type":"PISSN","@value":"00320889"}],"prism:publicationName":[{"@value":"Plant Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2004-12-01","prism:volume":"136","prism:number":"4","prism:startingPage":"4228","prism:endingPage":"4236"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/plphys/article-pdf/136/4/4228/37813941/plphys_v136_4_4228.pdf"}],"createdAt":"2004-12-11","modifiedAt":"2021-05-06","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002217131490560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of unique mechanisms for triterpene biosynthesis in\n            <i>Botryococcus braunii</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617488333824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Natural variation of diterpenoid phytoalexins in cultivated and wild rice species"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298754830601216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cold stress induces malformed tomato fruits by breaking the feedback loops of stem cell regulation in floral meristem"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044959831936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evolution of Labdane-Related Diterpene Synthases in Cereals"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204199108096","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"イネのジテルペン環化酵素遺伝子ファミリー"},{"@language":"en","@value":"Diterpene Cyclase Gene Family in Rice"},{"@language":"ja-Kana","@value":"イネ ノ ジテルペン カンカ コウソ イデンシ ファミリー"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206477908736","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Asymmetric Total Synthesis of ent-Sandaracopimaradiene, a Biosynthetic Intermediate of Oryzalexins"},{"@value":"Asymmetric Total Synthesis of<i>ent</i>-Sandaracopimaradiene, a Biosynthetic Intermediate of Oryzalexins"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206479873152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases"},{"@value":"Characterization of a rice gene family encoding type-A diterpene cyclase"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206480089856","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice"},{"@value":"Comparison of the Enzymatic Properties of<i>ent</i>-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303316992","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Transcriptional regulation of the biosynthesis of phytoalexin: A lesson from specialized metabolites in rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681454472192","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System"},{"@value":"Functional Identification of a Rice<i>ent</i>-Kaurene Oxidase, OsKO2, Using the<i>Pichia pastoris</i>Expression System"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681455526272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Recent Advances Regarding Diterpene Cyclase Genes in Higher Plants and Fungi"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681456513792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Biosynthesis of Phytoalexins and Regulatory Mechanisms of It in Rice"},{"@value":"Award Review : Biosynthesis of Phytoalexins and Regulatory Mechanisms of It in Rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681456599424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings"},{"@value":"Diterpene phytoalexins are biosynthesized in and exuded from roots of rice seedlings"}]},{"@id":"https://cir.nii.ac.jp/crid/1520573329912119552","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Functional Analysis of Eubacterial ent-Copalyl Diphosphate Synthase and Pimara-9(11),15-Diene Synthase with Unique Primary Sequences"},{"@language":"ja-Kana","@value":"Functional Analysis of Eubacterial ent Copalyl Diphosphate Synthase and Pimara 9 11 15 Diene Synthase with Unique Primary 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