{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137046263000960.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/jor.20566"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjor.20566"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jor.20566"}},{"identifier":{"@type":"PMID","@value":"18932231"}}],"dc:title":[{"@value":"Rotating three‐dimensional dynamic culture of adult human bone marrow‐derived cells for tissue engineering of hyaline cartilage"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>The method of constructing cartilage tissue from bone marrow‐derived cells in vitro is considered a valuable technique for hyaline cartilage regenerative medicine. Using a rotating wall vessel (RWV) bioreactor developed in a NASA space experiment, we attempted to efficiently construct hyaline cartilage tissue from human bone marrow‐derived cells without using a scaffold. Bone marrow aspirates were obtained from the iliac crest of nine patients during orthopedic operation. After their proliferation in monolayer culture, the adherent cells were cultured in the RWV bioreactor with chondrogenic medium for 2 weeks. Cells from the same source were cultured in pellet culture as controls. Histological and immunohistological evaluations (collagen type I and II) and quantification of glycosaminoglycan were performed on formed tissues and compared. The engineered constructs obtained using the RWV bioreactor showed strong features of hyaline cartilage in terms of their morphology as determined by histological and immunohistological evaluations. The glycosaminoglycan contents per µg DNA of the tissues were 10.01 ± 3.49 µg/µg DNA in the case of the RWV bioreactor and 6.27 ± 3.41 µg/µg DNA in the case of the pellet culture, and their difference was significant. The RWV bioreactor could provide an excellent environment for three‐dimensional cartilage tissue architecture that can promote the chondrogenic differentiation of adult human bone marrow‐derived cells. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 517–521, 2009</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137046263000967","@type":"Researcher","foaf:name":[{"@value":"Shinsuke Sakai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000963","@type":"Researcher","foaf:name":[{"@value":"Hajime Mishima"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000960","@type":"Researcher","foaf:name":[{"@value":"Tomoo Ishii"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000964","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Akaogi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000968","@type":"Researcher","foaf:name":[{"@value":"Tomokazu Yoshioka"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000965","@type":"Researcher","foaf:name":[{"@value":"Yoshimi Ohyabu"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000966","@type":"Researcher","foaf:name":[{"@value":"Fei Chang"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000961","@type":"Researcher","foaf:name":[{"@value":"Naoyuki Ochiai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046263000962","@type":"Researcher","foaf:name":[{"@value":"Toshimasa Uemura"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"07360266"},{"@type":"EISSN","@value":"1554527X"}],"prism:publicationName":[{"@value":"Journal of Orthopaedic Research"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2009-03-13","prism:volume":"27","prism:number":"4","prism:startingPage":"517","prism:endingPage":"521"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjor.20566"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jor.20566"}],"createdAt":"2008-10-17","modifiedAt":"2023-11-15","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Adult","dc:title":"Adult"},{"@id":"https://cir.nii.ac.jp/all?q=Rotation","dc:title":"Rotation"},{"@id":"https://cir.nii.ac.jp/all?q=Tissue%20Engineering","dc:title":"Tissue Engineering"},{"@id":"https://cir.nii.ac.jp/all?q=Bone%20Marrow%20Cells","dc:title":"Bone Marrow Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Differentiation","dc:title":"Cell Differentiation"},{"@id":"https://cir.nii.ac.jp/all?q=Middle%20Aged","dc:title":"Middle Aged"},{"@id":"https://cir.nii.ac.jp/all?q=Bioreactors","dc:title":"Bioreactors"},{"@id":"https://cir.nii.ac.jp/all?q=Hyaline%20Cartilage","dc:title":"Hyaline Cartilage"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Chondrogenesis","dc:title":"Chondrogenesis"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Glycosaminoglycans","dc:title":"Glycosaminoglycans"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004239983596160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690784068480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of implantation of three-dimensional engineered bone tissue with a vascular-like structure on repair of bone defects"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661480028288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing cartilage"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/jor.20566"},{"@type":"OPENAIRE","@value":"doi_dedup___::9c002345b1650a087e735de90e7dcc38"},{"@type":"CROSSREF","@value":"10.4236/msa.2013.48a010_references_DOI_SaktRlDXi1aY2f2ALnGjxO6g9Ul"},{"@type":"CROSSREF","@value":"10.1172/jci76443_references_DOI_SaktRlDXi1aY2f2ALnGjxO6g9Ul"},{"@type":"CROSSREF","@value":"10.1016/j.apsusc.2012.01.092_references_DOI_SaktRlDXi1aY2f2ALnGjxO6g9Ul"}]}