{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137046404943232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.0501034102"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.0501034102"}},{"identifier":{"@type":"NAID","@value":"30016302708"}}],"dc:title":[{"@value":"Recruitment of DNA methyltransferase I to DNA repair sites"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            In mammalian cells, the replication of genetic and epigenetic information is directly coupled; however, little is known about the maintenance of epigenetic information in DNA repair. Using a laser microirradiation system to introduce DNA lesions at defined subnuclear sites, we tested whether the major DNA methyltransferase (Dnmt1) or one of the two\n            <jats:italic>de novo</jats:italic>\n            methyltransferases (Dnmt3a, Dnmt3b) are recruited to sites of DNA repair\n            <jats:italic>in vivo</jats:italic>\n            . Time lapse microscopy of microirradiated mammalian cells expressing GFP-tagged Dnmt1, Dnmt3a, or Dnmt3b1 together with red fluorescent protein-tagged proliferating cell nuclear antigen (PCNA) revealed that Dnmt1 and PCNA accumulate at DNA damage sites as early as 1 min after irradiation in S and non-S phase cells, whereas recruitment of Dnmt3a and Dnmt3b was not observed. Deletion analysis showed that Dnmt1 recruitment was mediated by the PCNA-binding domain. These data point to a direct role of Dnmt1 in the restoration of epigenetic information during DNA repair.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380294721984219136","@type":"Researcher","foaf:name":[{"@value":"Oliver Mortusewicz"}],"jpcoar:affiliationName":[{"@value":"Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany; and Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294721984219139","@type":"Researcher","foaf:name":[{"@value":"Lothar Schermelleh"}],"jpcoar:affiliationName":[{"@value":"Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany; and Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294721984219138","@type":"Researcher","foaf:name":[{"@value":"Joachim Walter"}],"jpcoar:affiliationName":[{"@value":"Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany; and Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294721984219140","@type":"Researcher","foaf:name":[{"@value":"M. Cristina Cardoso"}],"jpcoar:affiliationName":[{"@value":"Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany; and Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294721984219137","@type":"Researcher","foaf:name":[{"@value":"Heinrich Leonhardt"}],"jpcoar:affiliationName":[{"@value":"Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany; and Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2005-06-13","prism:volume":"102","prism:number":"25","prism:startingPage":"8905","prism:endingPage":"8909"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.0501034102"}],"createdAt":"2005-06-15","modifiedAt":"2022-04-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050564285937521280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"Regulatory interaction between NBS1 and DNMT1 responding to DNA damage"}]},{"@id":"https://cir.nii.ac.jp/crid/1050845760757086336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability."}]},{"@id":"https://cir.nii.ac.jp/crid/1360285705857382528","@type":"Article","resourceType":"学術雑誌論文(journal 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