{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137046410312704.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.bbrc.2004.03.114"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X04006138?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X04006138?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"15081414"}},{"identifier":{"@type":"NAID","@value":"80016623685"}}],"dc:title":[{"@value":"Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells"}],"description":[{"notation":[{"@value":"Ca(2+) is the pivotal second messenger for induction of defense responses induced by treatment of pathogen-derived elicitor or microbial infection in plants. However, molecular bases for elicitor-induced generation of Ca(2+) signals (Ca(2+) transients) are largely unknown. We here identified cDNAs for putative voltage-dependent Ca(2+)-permeable channels, NtTPC1A and NtTPC1B, that are homologous to TPC1 (two pore channel) from suspension-cultured tobacco BY-2 cells. NtTPC1s complemented the growth of a Saccharomyces cerevisiae mutant defective in CCH1, a putative Ca(2+) channel, in a low Ca(2+) medium, suggesting that both products permeate Ca(2+) through the plasma membrane. Cosuppression of NtTPC1s in apoaequorin-expressing BY-2 cells resulted in inhibition of rise in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)) in response to sucrose and a fungal elicitor cryptogein, while it did not affect hypoosmotic shock-induced [Ca(2+)](cyt) increase. Cosuppression of NtTPC1s also caused suppression of cryptogein-induced programmed cell death and defense-related gene expression. These results suggest that NtTPC1s are involved in Ca(2+) mobilization induced by the cryptogein and sucrose, and have crucial roles in cryptogein-induced signal transduction pathway."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137046410312705","@type":"Researcher","foaf:name":[{"@value":"Yasuhiro Kadota"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312709","@type":"Researcher","foaf:name":[{"@value":"Takuya Furuichi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312704","@type":"Researcher","foaf:name":[{"@value":"Yoko Ogasawara"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312707","@type":"Researcher","foaf:name":[{"@value":"Tatsuaki Goh"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312708","@type":"Researcher","foaf:name":[{"@value":"Katsumi Higashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312832","@type":"Researcher","foaf:name":[{"@value":"Shoshi Muto"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046410312706","@type":"Researcher","foaf:name":[{"@value":"Kazuyuki Kuchitsu"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006291X"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/0006291X"}],"prism:publicationName":[{"@value":"Biochemical and Biophysical Research Communications"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2004-05","prism:volume":"317","prism:number":"3","prism:startingPage":"823","prism:endingPage":"830"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0006291X04006138?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0006291X04006138?httpAccept=text/plain"}],"createdAt":"2004-04-03","modifiedAt":"2025-09-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Nicotiana","dc:title":"Nicotiana"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Sequence%20Homology,%20Amino%20Acid","dc:title":"Sequence Homology, Amino Acid"},{"@id":"https://cir.nii.ac.jp/all?q=Algal%20Proteins","dc:title":"Algal Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Saccharomyces%20cerevisiae","dc:title":"Saccharomyces cerevisiae"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Fungal%20Proteins","dc:title":"Fungal Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Calcium","dc:title":"Calcium"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Calcium%20Channels","dc:title":"Calcium Channels"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Primers","dc:title":"DNA Primers"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215743016704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"New Insights into the Transport Mechanisms in Plant Vacuoles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234737979904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cryptogein-Induced Cell Cycle Arrest at G2 Phase is Associated with Inhibition of Cyclin-Dependent Kinases, Suppression of Expression of Cell Cycle-Related Genes and Protein Degradation in Synchronized Tobacco BY-2 Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168833467392","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean Macroalga Nitellopsis obtusa and the Early Terrestrial Liverwort Marchantia polymorpha"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298757176701312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Fluorescent Auxin Analogs Report Two Auxin Binding Sites with Different Subcellular Distribution and Affinities: A Cue for Non-Transcriptional Auxin Signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165304209408","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846642074599424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Regulation of a Proteinaceous Elicitor-induced Ca2+ Influx and Production of Phytoalexins by a Putative Voltage-gated Cation Channel, OsTPC1, in Cultured Rice Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660734655744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"On the cellular site of two‐pore channel <scp>TPC</scp>1 action in the Poaceae"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848664888916352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracellular localization and physiological function of a rice Ca<sup>2+</sup>-permeable channel OsTPC1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593763186688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional Analyses of the Two Distinctive Types of Two-Pore Channels and the Slow Vacuolar Channel in\n                    <i>Marchantia polymorpha</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204328662272","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Controlled salicylic acid levels and corresponding signaling mechanisms in plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679304731904","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice"},{"@value":"Evidence for the plasma membrane localization of a putative voltage-dependent Ca〔2+〕 channel, OsTPC1, in rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679306115328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Regulation of xylanase elicitor-induced expression of defense-related genes involved in phytoalexin biosynthesis by a cation channel OsTPC1 in suspension-cultured rice cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679645361664","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Roles of the Putative Voltage-Gated Ca<sup>2+</sup> Permeable Channels, the TPC1 Family, in Plant Stress Signaling"},{"@value":"Roles of the Putative Voltage-Gated Ca〔2+〕 Permeable Channels, the TPC1 Family, in Plant Stress Signaling"},{"@language":"ja-Kana","@value":"Roles of the Putative Voltage Gated Ca 2 Permeable Channels the TPC1 Family in Plant Stress Signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680062951552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"植物のストレス応答情報伝達におけるカルシウムイオンの役割とその動員の分子機構"},{"@language":"en","@value":"Physiological roles and molecular mechanisms for stress-induced Ca^<2+> mobilization in plants"},{"@language":"ja-Kana","@value":"ショクブツ ノ ストレス オウトウ ジョウホウ デンタツ ニ オケル カルシウム イオン ノ ヤクワリ ト ソノ ドウイン ノ ブンシ キコウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1520854804868613376","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells"},{"@language":"ja-Kana","@value":"Cell Cycle Dependence of Elicitor induced Signal Transduction in Tobacco BY 2 Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1520854805541799168","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells"},{"@language":"ja-Kana","@value":"Cryptogein induced cell cycle arrest at G2 phase is associated with inhibition of cyclin dependent kinases suppression of expression of cell cycle related genes and protein degradation in synchronized tobacco BY 2 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1522825130831529984","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"The fou2 gain-of-function allele and the wild-type allele of Two Pore Channel 1 contribute to different extents or by different mechanisms to defense gene expression in arabidopsis"},{"@language":"ja-Kana","@value":"The fou2 gain of function allele and the wild type allele of Two Pore Channel 1 contribute to different extents or by different mechanisms to defense gene expression in arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1523106605594196224","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: in vivo imaging of hypersensitive cell death in tobacco BY-2 cells"},{"@language":"ja-Kana","@value":"Elicitor induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death in vivo imaging of hypersensitive cell death in tobacco BY 2 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1523951030567129216","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants"},{"@language":"ja-Kana","@value":"Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2004.03.114"},{"@type":"CIA","@value":"80016623685"},{"@type":"OPENAIRE","@value":"doi_dedup___::15d8d255487f8a86fff8f5a4fe079e71"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcr042_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.1016/b978-0-12-407695-2.00009-3_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.3390/plants10040647_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.3390/ijms23158593_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.1007/s10265-011-0408-z_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.21.319_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.2480/agrmet.1109_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.1074/jbc.m111.337659_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.1111/nph.12402_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.4161/psb.22086_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.22.235_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcab176_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0805b_references_DOI_On7adtC7ptqsvmJ6oqAcFpGo7r5"}]}