{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137046458080256.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.aquatox.2008.04.009"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0166445X08001264?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0166445X08001264?httpAccept=text/plain"}},{"identifier":{"@type":"HANDLE","@value":"10722/92706"}}],"dc:title":[{"@value":"Real-time PCR array to study effects of chemicals on the Hypothalamic–Pituitary–Gonadal axis of the Japanese medaka"}],"description":[{"notation":[{"@value":"This paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic–pituitary–gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR® Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17-ethinylestradiol (EE2) and the anabolic androgen, 17-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-monthold medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7 d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-˛ expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action. © 2008 Elsevier B.V. All rights reserved."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381137046458080256","@type":"Researcher","foaf:name":[{"@value":"Xiaowei Zhang"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080260","@type":"Researcher","foaf:name":[{"@value":"Markus Hecker"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080266","@type":"Researcher","foaf:name":[{"@value":"June-Woo Park"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080263","@type":"Researcher","foaf:name":[{"@value":"Amber R. Tompsett"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080258","@type":"Researcher","foaf:name":[{"@value":"John Newsted"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080264","@type":"Researcher","foaf:name":[{"@value":"Kei Nakayama"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080257","@type":"Researcher","foaf:name":[{"@value":"Paul D. Jones"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080261","@type":"Researcher","foaf:name":[{"@value":"Doris Au"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080259","@type":"Researcher","foaf:name":[{"@value":"Richard Kong"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080262","@type":"Researcher","foaf:name":[{"@value":"Rudolf S.S. Wu"}]},{"@id":"https://cir.nii.ac.jp/crid/1381137046458080265","@type":"Researcher","foaf:name":[{"@value":"John P. Giesy"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0166445X"}],"prism:publicationName":[{"@value":"Aquatic Toxicology"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2008-07","prism:volume":"88","prism:number":"3","prism:startingPage":"173","prism:endingPage":"182"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0166445X08001264?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0166445X08001264?httpAccept=text/plain"}],"createdAt":"2008-04-25","modifiedAt":"2018-12-29","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Fish","dc:title":"Fish"},{"@id":"https://cir.nii.ac.jp/all?q=Fecundity","dc:title":"Fecundity"},{"@id":"https://cir.nii.ac.jp/all?q=Steroidogenesis","dc:title":"Steroidogenesis"},{"@id":"https://cir.nii.ac.jp/all?q=Expression%20profile","dc:title":"Expression profile"},{"@id":"https://cir.nii.ac.jp/all?q=616","dc:title":"616"},{"@id":"https://cir.nii.ac.jp/all?q=Endocrine","dc:title":"Endocrine"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002214674751744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"17β‐Trenbolone exposure programs metabolic dysfunction in larval medaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182005399168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Gene expression profiles in the testis associated with testis–ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869854364845312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Non‐Phthalate Plasticizer Bis(2‐ethylhexyl) Sebacate Induces Testis‐Ova Formation and Suppresses Reproduction in Japanese Medaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1521417755182178048","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular Cloning and Characterization of the Expression Profiles of Vitellogenin Transcripts in the Dojo Loach (Misgurnus anguillicaudatus) in Response to 17α-ethinylestradiol and 17β-estradiol Administration"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.aquatox.2008.04.009"},{"@type":"OPENAIRE","@value":"doi_dedup___::fd0622b4d0b273b5320215ab266f950f"},{"@type":"CROSSREF","@value":"10.1002/tox.22158_references_DOI_THu43crTUX8MHwVYRFdgzOKz3RR"},{"@type":"CROSSREF","@value":"10.2108/zs130223_references_DOI_THu43crTUX8MHwVYRFdgzOKz3RR"},{"@type":"CROSSREF","@value":"10.1016/j.chemosphere.2011.12.047_references_DOI_THu43crTUX8MHwVYRFdgzOKz3RR"},{"@type":"CROSSREF","@value":"10.1002/jat.4794_references_DOI_THu43crTUX8MHwVYRFdgzOKz3RR"}]}