{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361137046494359552.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.1014194107"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.1014194107"}}],"dc:title":[{"@value":"Human tumor virus utilizes exosomes for intercellular communication"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The Epstein–Barr virus (EBV) latent membrane protein 1 (LMP1) is expressed in multiple human malignancies and has potent effects on cell growth. It has been detected in exosomes and shown to inhibit immune function. Exosomes are small secreted cellular vesicles that contain proteins, mRNAs, and microRNAs (miRNAs). When produced by malignant cells, they can promote angiogenesis, cell proliferation, tumor-cell invasion, and immune evasion. In this study, exosomes released from nasopharyngeal carcinoma (NPC) cells harboring latent EBV were shown to contain LMP1, signal transduction molecules, and virus-encoded miRNAs. Exposure to these NPC exosomes activated the ERK and AKT signaling pathways in the recipient cells. Interestingly, NPC exosomes also contained viral miRNAs, several of which were enriched in comparison with their intracellular levels. LMP1 induces expression of the EGF receptor in an EBV-negative epithelial cell line, and exosomes produced by these cells also contain high levels of EGF receptor in exosomes. These findings suggest that the effects of EBV and LMP1 on cellular expression also modulate exosome content and properties. The exosomes may manipulate the tumor microenvironment to influence the growth of neighboring cells through the intercellular transfer of LMP1, signaling molecules, and viral miRNAs.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380857595432252931","@type":"Researcher","foaf:name":[{"@value":"David G. Meckes"}],"jpcoar:affiliationName":[{"@value":"The Lineberger Comprehensive Cancer Center, and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857595432252930","@type":"Researcher","foaf:name":[{"@value":"Kathy H. Y. Shair"}],"jpcoar:affiliationName":[{"@value":"The Lineberger Comprehensive Cancer Center, and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857595432252800","@type":"Researcher","foaf:name":[{"@value":"Aron R. Marquitz"}],"jpcoar:affiliationName":[{"@value":"The Lineberger Comprehensive Cancer Center, and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857595432252801","@type":"Researcher","foaf:name":[{"@value":"Che-Pei Kung"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology–Immunology, University of North Carolina, Chapel Hill, NC 27599"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857595432252929","@type":"Researcher","foaf:name":[{"@value":"Rachel H. Edwards"}],"jpcoar:affiliationName":[{"@value":"The Lineberger Comprehensive Cancer Center, and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857595432252928","@type":"Researcher","foaf:name":[{"@value":"Nancy Raab-Traub"}],"jpcoar:affiliationName":[{"@value":"The Lineberger Comprehensive Cancer Center, and"},{"@value":"Department of Microbiology–Immunology, University of North Carolina, Chapel Hill, NC 27599"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2010-11-08","prism:volume":"107","prism:number":"47","prism:startingPage":"20370","prism:endingPage":"20375"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.1014194107"}],"createdAt":"2010-11-09","modifiedAt":"2022-04-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050025031476703872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1050587981433165824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Exosomes Derived from Epstein-Barr Virus-Infected Cells Are Internalized via Caveola-Dependent Endocytosis and Promote Phenotypic Modulation in Target Cells"},{"@value":"Exosomes derived from Epstein-Barr virus-infected cells are internalized via caveolae-dependent endocytosis and promote phenotypic modulation in target cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1050861770482413312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002219914691456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004236406322432","@type":"Article","resourceType":"学術雑誌論文(journal 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