{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418518614473344.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1161/01.cir.88.6.2849"}},{"identifier":{"@type":"URI","@value":"https://www.ahajournals.org/doi/pdf/10.1161/01.CIR.88.6.2849"}},{"identifier":{"@type":"NAID","@value":"10005028379"}}],"dc:title":[{"@value":"Identification of functional angiotensin II receptors on rat cardiac fibroblasts."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:sec>\n            <jats:title>BACKGROUND</jats:title>\n            <jats:p>Cardiac hypertrophy results in an increased deposition of the extracellular matrix (ECM) proteins fibronectin and collagen. Recent evidence indicates that angiotensin II (Ang II) might have an important role in the development of myocardial fibrosis accompanying cardiac hypertrophy. We sought to determine whether fibroblasts of cardiac origin (isolated from neonatal and adult animals) express receptors for Ang II and to examine the ability of this peptide to regulate fibronectin and collagen gene expression in a cultured adult cardiac fibroblast cell preparation.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>METHODS AND RESULTS</jats:title>\n            <jats:p>Binding of 125I-Ang II to both neonatal and adult cardiac fibroblasts in culture was specific, reversible, and saturable, with the receptor evenly distributed over the cell population. Competition binding experiments with receptor-specific antagonists indicate that Ang II receptors found on both fibroblast types were of the AT1 subtype. Analysis of mRNA levels for the AT1 receptor indicates that adult cardiac fibroblasts express higher levels of the message than neonatal fibroblasts or cardiac myocytes. Addition of 10(-9) mol/L Ang II to adult cardiac fibroblasts resulted in an induction of ECM proteins above control levels, as determined through Northern blots and total collagen assays.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>CONCLUSIONS</jats:title>\n            <jats:p>Results from this study indicate that neonatal and adult rat cardiac fibroblasts in culture express AT1 receptors for Ang II. Ang II stimulation of AT1 receptors results in an increased gene expression for ECM proteins. These data suggest that Ang II might have important regulatory roles over cardiac fibroblast function under normal and pathological conditions.</jats:p>\n          </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1583387449166941568","@type":"Researcher","foaf:name":[{"@value":"F J Villarreal"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, University of California San Diego."}]},{"@id":"https://cir.nii.ac.jp/crid/1381418518614473345","@type":"Researcher","foaf:name":[{"@value":"N N Kim"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, University of California San Diego."}]},{"@id":"https://cir.nii.ac.jp/crid/1381418518614473348","@type":"Researcher","foaf:name":[{"@value":"G D Ungab"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, University of California San Diego."}]},{"@id":"https://cir.nii.ac.jp/crid/1381418518614473347","@type":"Researcher","foaf:name":[{"@value":"M P Printz"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, University of California San Diego."}]},{"@id":"https://cir.nii.ac.jp/crid/1381418518614473344","@type":"Researcher","foaf:name":[{"@value":"W H Dillmann"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, University of California San Diego."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00097322"},{"@type":"EISSN","@value":"15244539"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00097322"}],"prism:publicationName":[{"@value":"Circulation"}],"dc:publisher":[{"@value":"Ovid Technologies (Wolters Kluwer Health)"}],"prism:publicationDate":"1993-12","prism:volume":"88","prism:number":"6","prism:startingPage":"2849","prism:endingPage":"2861"},"reviewed":"false","url":[{"@id":"https://www.ahajournals.org/doi/pdf/10.1161/01.CIR.88.6.2849"}],"createdAt":"2012-06-12","modifiedAt":"2024-05-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233976229504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Pertussis Toxin Up-regulates Angiotensin Type 1 Receptors through Toll-like Receptor 4-mediated Rac Activation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565168966388224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Pathophysiology and therapeutic potential of cardiac fibrosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205037951488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Association Between Angiotensin I-Converting Enzyme Gene Insertion/Deletion Polymorphism and Risk of Rheumatic Heart Disease"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679263786368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Inhibitory Effect of Tranilast on Hypertrophic Collagen Production in the Spontaneously Hypertensive Rat Heart."},{"@language":"ja-Kana","@value":"Inhibitory Effect of Tranilast on Hyper"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680079118976","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mechanisms of Remodelling  A Question of Life (Stem Cell Production) and Death (Myocyte Apoptosis)"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698599306682880","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"高血圧症における左室拡張障害に対するエナラプリルの効果について-心エコー図, パルス・ドプラ法による検討-"},{"@language":"en","@value":"Effects of Enalapril on Left Ventricular Diastolic Property in Hypertensive Disease:A Study of M-Mode and Pulsed Doppler Echocardiography"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1161/01.cir.88.6.2849"},{"@type":"CIA","@value":"10005028379"},{"@type":"CROSSREF","@value":"10.1074/jbc.m109.076232_references_DOI_qeZiJDb1ua1HyZc7MxI1kYKNT1"},{"@type":"CROSSREF","@value":"10.1186/s41232-017-0046-5_references_DOI_qeZiJDb1ua1HyZc7MxI1kYKNT1"},{"@type":"CROSSREF","@value":"10.1536/jhj.45.949_references_DOI_qeZiJDb1ua1HyZc7MxI1kYKNT1"},{"@type":"CROSSREF","@value":"10.1253/circj.cj-09-0573_references_DOI_qeZiJDb1ua1HyZc7MxI1kYKNT1"},{"@type":"CROSSREF","@value":"10.1254/jjp.78.161_references_DOI_qeZiJDb1ua1HyZc7MxI1kYKNT1"}]}