{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418519012034432.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1083/jcb.100.4.1325"}},{"identifier":{"@type":"URI","@value":"https://rupress.org/jcb/article-pdf/100/4/1325/1463701/1325.pdf"}}],"dc:title":[{"@value":"An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The eggs of most or all animals are thought to be activated after fertilization by a transient increase in free cytosolic Ca2+ concentration ([Ca2+]i). We have applied Ca2+-selective microelectrodes to detect such an increase in fertilized eggs of the frog, Xenopus laevis. As observed with an electrode in the animal hemisphere, [Ca2+]i increased from 0.4 to 1.2 microM over the course of 2 min after fertilization, and returned to its original value during the next 10 min. No further changes in [Ca2+]i were detected through the first cleavage division. In eggs impaled with two Ca2+ electrodes, the Ca2+ pulse was observed to travel as a wave from the animal to the vegetal hemisphere, propagating at a rate of approximately 10 microns/s across the animal hemisphere. The apparent delay between the start of the fertilization potential and initiation of the Ca2+ wave at the sperm entry site as approximately 1 min. Through these observations describe only the behavior of subcortical [Ca2+]i, we suggest that our data represent the subcortical extension of the cortical Ca2+ wave thought to trigger cortical granule exocytosis, and we present evidence that both the timing and magnitude of the Ca2+ pulse we observed are consistent with this identity. This first quantification of subcortical [Ca2+]i during fertilization indicates that the Ca2+ transient is available to regulate processes (e.g., protein synthesis) in the subcortical cytosol.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380285705855036042","@type":"Researcher","foaf:name":[{"@value":"W B Busa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519012034304","@type":"Researcher","foaf:name":[{"@value":"R Nuccitelli"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219525"},{"@type":"EISSN","@value":"15408140"}],"prism:publicationName":[{"@value":"The Journal of cell biology"}],"dc:publisher":[{"@value":"Rockefeller University Press"}],"prism:publicationDate":"1985-04-01","prism:volume":"100","prism:number":"4","prism:startingPage":"1325","prism:endingPage":"1329"},"reviewed":"false","url":[{"@id":"https://rupress.org/jcb/article-pdf/100/4/1325/1463701/1325.pdf"}],"createdAt":"2004-05-15","modifiedAt":"2023-07-22","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050306506453070080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Fertilization 2: Polyspermic Fertilization"}]},{"@id":"https://cir.nii.ac.jp/crid/1050564285607982976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Activation of Xenopus Eggs by Cynops Sperm Extract is Dependent upon Both Extra-and Intra-Cellular Ca Activities"},{"@language":"ja-Kana","@value":"Activation of Xenopus eggs by Cynops sp"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691807304320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracellular calcium signal at the leading edge regulates mesodermal sheet migration during Xenopus gastrulation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298757422696704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"SPERM FACTORS AND EGG ACTIVATION: Divergent sperm factors for egg activation in amphibian fertilization"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565170630762624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Calcium Signaling and Meiotic Exit at Fertilization in  Xenopus Egg"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567180185219200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The electrical block to polyspermy induced by an intracellular Ca<sup>2+</sup> increase at fertilization of the clawed frogs, <i>Xenopus laevis</i> and <i>Xenopus tropicalis</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707375741440","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly in <i>Xenopus</i> oocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1524232505207248512","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mechanisms of Egg Activation and Polyspermy Block in Amphibians and Comparative Aspects with Fertilization in Other Vertebrates"},{"@language":"ja-Kana","@value":"Mechanisms of Egg Activation and Polyspermy Block in Amphibians and Comparative Aspects with Fertilization in Other Vertebrates"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1083/jcb.100.4.1325"},{"@type":"CROSSREF","@value":"10.1038/s41598-018-20747-w_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.1007/978-981-10-3975-1_7_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.3390/ijms151018659_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.1002/mrd.23115_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.1091/mbc.e22-06-0218_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.1530/rep-21-0480_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.2108/zsj.17.699_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"},{"@type":"CROSSREF","@value":"10.2108/zsj.12.573_references_DOI_IN2P54qMyue6AX46pmGw0myKWBy"}]}