{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418519246541568.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.040337597"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.040337597"}},{"identifier":{"@type":"PMID","@value":"10688880"}},{"identifier":{"@type":"NAID","@value":"30016225663"}}],"dc:title":[{"@value":"Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor:<i>In vivo</i>expression and antitumor activity"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Neuropilin-1 (NRP1) is a 130-kDa transmembrane receptor for semaphorins, mediators of neuronal guidance, and for vascular endothelial growth factor 165 (VEGF<jats:sub>165</jats:sub>), an angiogenesis factor. A 2.2-kb truncated NRP1 cDNA was cloned that encodes a 644-aa soluble NRP1 (sNRP1) isoform containing just the a/CUB and b/coagulation factor homology extracellular domains of NRP1. sNRP1 is secreted by cells as a 90-kDa protein that binds VEGF<jats:sub>165</jats:sub>, but not VEGF<jats:sub>121</jats:sub>. It inhibits<jats:sup>125</jats:sup>I-VEGF<jats:sub>165</jats:sub>binding to endothelial and tumor cells and VEGF<jats:sub>165</jats:sub>-induced tyrosine phosphorylation of KDR in endothelial cells. The 3′ end of sNRP1 cDNA contains a unique, 28-bp intron-derived sequence that is absent in full-length NRP1 cDNA. Using a probe corresponding to this unique sequence, sNRP1 mRNA could be detected by<jats:italic>in situ</jats:italic>hybridization differentially from full-length NRP1 mRNA, for example, in cells of liver, kidney, skin, and breast. Analysis of blood vessels<jats:italic>in situ</jats:italic>showed that NRP1, but not sNRP1, was expressed. sNRP1 was functional<jats:italic>in vivo</jats:italic>. Unlike control tumors, tumors of rat prostate carcinoma cells expressing recombinant sNRP1 were characterized by extensive hemorrhage, damaged vessels, and apoptotic tumor cells. These results demonstrate the existence of a naturally occurring, soluble NRP1 that is expressed differently from intact NRP1 and that appears to be a VEGF<jats:sub>165</jats:sub>antagonist.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380576120389940608","@type":"Researcher","foaf:name":[{"@value":"Michael L. Gagnon"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940480","@type":"Researcher","foaf:name":[{"@value":"Diane R. Bielenberg"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940609","@type":"Researcher","foaf:name":[{"@value":"Ze'ev Gechtman"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940483","@type":"Researcher","foaf:name":[{"@value":"Hua-Quan Miao"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940484","@type":"Researcher","foaf:name":[{"@value":"Seiji Takashima"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940482","@type":"Researcher","foaf:name":[{"@value":"Shay Soker"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120389940481","@type":"Researcher","foaf:name":[{"@value":"Michael Klagsbrun"}],"jpcoar:affiliationName":[{"@value":"Departments of Surgical Research, Pathology, and Urology, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115; and First Department of Medicine, Osaka University Hospital, Yamadaoka 2-2, Suita, Osaka 565, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2000-02-25","prism:volume":"97","prism:number":"6","prism:startingPage":"2573","prism:endingPage":"2578"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.040337597"}],"createdAt":"2002-07-26","modifiedAt":"2024-01-04","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factor%20A","dc:title":"Vascular Endothelial Growth Factor A"},{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Complementary","dc:title":"DNA, Complementary"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Antineoplastic%20Agents","dc:title":"Antineoplastic Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Apoptosis","dc:title":"Apoptosis"},{"@id":"https://cir.nii.ac.jp/all?q=Nerve%20Tissue%20Proteins","dc:title":"Nerve Tissue Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=CHO%20Cells","dc:title":"CHO Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelial%20Growth%20Factors","dc:title":"Endothelial Growth Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Cricetinae","dc:title":"Cricetinae"},{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Cells,%20Cultured","dc:title":"Tumor Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Isoforms","dc:title":"Protein Isoforms"},{"@id":"https://cir.nii.ac.jp/all?q=Tissue%20Distribution","dc:title":"Tissue Distribution"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphorylation","dc:title":"Phosphorylation"},{"@id":"https://cir.nii.ac.jp/all?q=Lymphokines","dc:title":"Lymphokines"},{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factors","dc:title":"Vascular Endothelial Growth Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Prostatic%20Neoplasms","dc:title":"Prostatic Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Blotting,%20Northern","dc:title":"Blotting, Northern"},{"@id":"https://cir.nii.ac.jp/all?q=Introns","dc:title":"Introns"},{"@id":"https://cir.nii.ac.jp/all?q=Neuropilin-1","dc:title":"Neuropilin-1"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Proteins","dc:title":"Recombinant Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Rats","dc:title":"Rats"},{"@id":"https://cir.nii.ac.jp/all?q=Liver","dc:title":"Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Tyrosine","dc:title":"Tyrosine"},{"@id":"https://cir.nii.ac.jp/all?q=Neoplasm%20Transplantation","dc:title":"Neoplasm Transplantation"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Binding","dc:title":"Protein Binding"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004230139211520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Long isoform of VEGF stimulates cell migration of breast cancer by filopodia formation via NRP1/ARHGAP17/Cdc42 regulatory network"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848655066678144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Adjuvant effects of formalin‐inactivated HSV through activation of dendritic cells and inactivation of myeloid‐derived suppressor cells in cancer immunotherapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1390017345590003968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"NRP1 Induces 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