{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418519251246976.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jb.144.2.569-578.1980"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/jb.144.2.569-578.1980"}}],"dc:title":[{"@value":"Ethanol Production by Thermophilic Bacteria: Relationship Between Fermentation Product Yields of and Catabolic Enzyme Activities in\n            <i>Clostridium thermocellum</i>\n            and\n            <i>Thermoanaerobium brockii</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            Significant quantitative differences in end-product yields by two strains of\n            <jats:italic>Clostridium thermocellum</jats:italic>\n            and one strain of\n            <jats:italic>Thermoanaerobium brockii</jats:italic>\n            were observed during cellobiose fermentation. Most notably, the ethanol/H\n            <jats:sub>2</jats:sub>\n            and lactate/acetate ratios were drastically higher for\n            <jats:italic>T. brockii</jats:italic>\n            as compared with\n            <jats:italic>C. thermocellum</jats:italic>\n            strains LQRI and AS39. Exogenous H\n            <jats:sub>2</jats:sub>\n            addition (0.4 to 1.0 atm) during culture growth increased the ethanol/acetate ratio of both\n            <jats:italic>T. brockii</jats:italic>\n            and AS39 but had no effect on LQRI. All strains had an operative Embden-Meyerhof glycolytic pathway and displayed catabolic activities of fructose-1,6-diphosphate–activated lactate dehydrogenase, coenzyme A acetylating pyruvate and acetaldehyde dehydrogenase, hydrogenase, ethanol dehydrogenase, and acetate kinase. Enzyme kinetic properties (apparent\n            <jats:italic>\n              K\n              <jats:sub>m</jats:sub>\n            </jats:italic>\n            ,\n            <jats:italic>V</jats:italic>\n            <jats:sub>max</jats:sub>\n            , and Q\n            <jats:sub>10</jats:sub>\n            values) and the specificity of electron donors/acceptors for different oxidoreductases involved in pyruvate conversion to fermentation products were compared in the three strains. Both species contained ferredoxin-linked pyruvate dehydrogenase and pyridine nucleotide oxidoreductases. Ferredoxin-nicotinamide adenine dinucleotide (NAD) reductase activity was significantly higher in\n            <jats:italic>T. brockii</jats:italic>\n            than in AS39 and was not detectable in LQRI. H\n            <jats:sub>2</jats:sub>\n            production and hydrogenase activity were inversely related to ferredoxin-NAD reductase activity in the three strains. Ferredoxin-NAD phosphate reductase activity was present in cell extracts of both species. Alcohol dehydrogenase activity in\n            <jats:italic>C. thermocellum</jats:italic>\n            was NAD dependent, unidirectional, and inhibited by low concentrations of NAD and ethanol. Ethanol dehydrogenase activity of\n            <jats:italic>T. brockii</jats:italic>\n            was both NAD and NADP linked, reversible, and not inhibited by low levels of reaction products. The high lactate yield of\n            <jats:italic>T. brockii</jats:italic>\n            correlated with increased fructose-1,6-diphosphate. The relation of catabolic enzyme activity and quantitative differences in intracellular electron flow and fermentation product yields of these thermophilic bacteria is discussed.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418519251246977","@type":"Researcher","foaf:name":[{"@value":"R. Lamed"}],"jpcoar:affiliationName":[{"@value":"Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519251246976","@type":"Researcher","foaf:name":[{"@value":"J. G. Zeikus"}],"jpcoar:affiliationName":[{"@value":"Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219193"},{"@type":"EISSN","@value":"10985530"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00219193"}],"prism:publicationName":[{"@value":"Journal of Bacteriology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"1980-11","prism:volume":"144","prism:number":"2","prism:startingPage":"569","prism:endingPage":"578"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/jb.144.2.569-578.1980"}],"createdAt":"2020-01-03","modifiedAt":"2021-07-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360284919608368640","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The anaerobic conversion of methanol under thermophilic conditions: pH and bicarbonate dependence"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846644090636288","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Anaerobic Conversion of Methanol under Thermophilic Conditions: pH and Bicarbonate Dependence"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679322253440","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ethanol Production Efficiency of an Anaerobic Hemicellulolytic Thermophilic Bacterium, Strain NTOU1, Isolated from a Marine Shallow Hydrothermal Vent in Taiwan"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jb.144.2.569-578.1980"},{"@type":"CROSSREF","@value":"10.1264/jsme2.me10202_references_DOI_OVBtWmvbHYfQ28yiFiZmWDTDXny"},{"@type":"CROSSREF","@value":"10.1263/jbb.96.213_references_DOI_OVBtWmvbHYfQ28yiFiZmWDTDXny"},{"@type":"CROSSREF","@value":"10.1016/s1389-1723(03)80184-6_references_DOI_OVBtWmvbHYfQ28yiFiZmWDTDXny"}]}