Plasma membrane—endoplasmic reticulum contact sites regulate phosphatidylcholine synthesis

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  • Jesse T Chao
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia Vancouver British Columbia Canada V6T 1Z3
  • Barry P Young
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia Vancouver British Columbia Canada V6T 1Z3
  • Shabnam Tavassoli
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia Vancouver British Columbia Canada V6T 1Z3
  • Ruud C Cox
    Department of Membrane Biochemistry and Biophysics, Bijvoet Center and Institute of Biomembranes, Utrecht University 3584 CH Utrecht The Netherlands
  • William A Prinz
    Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Bethesda Maryland 20892 USA
  • Christopher J R Loewen
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia Vancouver British Columbia Canada V6T 1Z3
  • Anton I P M de Kroon
    Department of Membrane Biochemistry and Biophysics, Bijvoet Center and Institute of Biomembranes, Utrecht University 3584 CH Utrecht The Netherlands

Bibliographic Information

Published
2013-03-22
Rights Information
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1038/embor.2013.36
Publisher
Springer Science and Business Media LLC

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Description

<jats:p> Synthesis of phospholipids, sterols and sphingolipids is thought to occur at contact sites between the endoplasmic reticulum (ER) and other organelles because many lipid‐synthesizing enzymes are enriched in these contacts. In only a few cases have the enzymes been localized to contacts <jats:italic>in vivo</jats:italic> and in no instances have the contacts been demonstrated to be required for enzyme function. Here, we show that plasma membrane (PM)—ER contact sites in yeast are required for phosphatidylcholine synthesis and regulate the activity of the phosphatidylethanolamine <jats:italic>N</jats:italic> ‐methyltransferase enzyme, Opi3. Opi3 activity requires Osh3, which localizes to PM–ER contacts where it might facilitate <jats:italic>in trans</jats:italic> catalysis by Opi3. Thus, membrane contact sites provide a structural mechanism to regulate lipid synthesis. </jats:p>

Journal

  • EMBO reports

    EMBO reports 14 (5), 434-440, 2013-03-22

    Springer Science and Business Media LLC

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