{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418519819081600.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajpheart.2001.280.2.h746"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.2001.280.2.H746"}}],"dc:title":[{"@value":"Upregulated<i>TRP</i>and enhanced capacitative Ca<sup>2+</sup>entry in human pulmonary artery myocytes during proliferation"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>A rise in cytosolic Ca<jats:sup>2+</jats:sup>concentration ([Ca<jats:sup>2+</jats:sup>]<jats:sub>cyt</jats:sub>) due to Ca<jats:sup>2+</jats:sup>release from intracellular Ca<jats:sup>2+</jats:sup>stores and Ca<jats:sup>2+</jats:sup>influx through plasmalemmal Ca<jats:sup>2+</jats:sup>channels plays a critical role in mitogen-mediated cell growth. Depletion of intracellular Ca<jats:sup>2+</jats:sup>stores triggers capacitative Ca<jats:sup>2+</jats:sup>entry (CCE), a mechanism involved in maintaining Ca<jats:sup>2+</jats:sup>influx and refilling intracellular Ca<jats:sup>2+</jats:sup>stores. Transient receptor potential ( TRP) genes have been demonstrated to encode the store-operated Ca<jats:sup>2+</jats:sup>channels that are activated by Ca<jats:sup>2+</jats:sup>store depletion. In this study, we examined whether CCE, activity of store-operated Ca<jats:sup>2+</jats:sup>channels, and human TRP1 ( hTRP1) expression are essential in human pulmonary arterial smooth muscle cell (PASMC) proliferation. Chelation of extracellular Ca<jats:sup>2+</jats:sup>and depletion of intracellularly stored Ca<jats:sup>2+</jats:sup>inhibited PASMC growth in media containing serum and growth factors. Resting [Ca<jats:sup>2+</jats:sup>]<jats:sub>cyt</jats:sub>as well as the increases in [Ca<jats:sup>2+</jats:sup>]<jats:sub>cyt</jats:sub>due to Ca<jats:sup>2+</jats:sup>release and CCE were all significantly greater in proliferating PASMC than in growth-arrested cells. Consistently, whole cell inward currents activated by depletion of intracellular Ca<jats:sup>2+</jats:sup>stores and the mRNA level of hTRP1 were much greater in proliferating PASMC than in growth-arrested cells. These results suggest that elevated [Ca<jats:sup>2+</jats:sup>]<jats:sub>cyt</jats:sub>and intracellularly stored [Ca<jats:sup>2+</jats:sup>] play an important role in pulmonary vascular smooth muscle cell growth. CCE, potentially via hTRP1-encoded Ca<jats:sup>2+</jats:sup>-permeable channels, may be an important mechanism required to maintain the elevated [Ca<jats:sup>2+</jats:sup>]<jats:sub>cyt</jats:sub>and stored [Ca<jats:sup>2+</jats:sup>] in human PASMC during proliferation.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418519819081607","@type":"Researcher","foaf:name":[{"@value":"Vera A. Golovina"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081605","@type":"Researcher","foaf:name":[{"@value":"Oleksandr Platoshyn"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081601","@type":"Researcher","foaf:name":[{"@value":"Colleen L. Bailey"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081604","@type":"Researcher","foaf:name":[{"@value":"Jian Wang"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081602","@type":"Researcher","foaf:name":[{"@value":"Alisa Limsuwan"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081606","@type":"Researcher","foaf:name":[{"@value":"Michele Sweeney"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081600","@type":"Researcher","foaf:name":[{"@value":"Lewis J. Rubin"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519819081603","@type":"Researcher","foaf:name":[{"@value":"Jason X.-J. Yuan"}],"jpcoar:affiliationName":[{"@value":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of California, San Diego, California 92103"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03636135"},{"@type":"EISSN","@value":"15221539"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Heart and Circulatory Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2001-02-01","prism:volume":"280","prism:number":"2","prism:startingPage":"H746","prism:endingPage":"H755"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.2001.280.2.H746"}],"createdAt":"2017-12-22","modifiedAt":"2022-08-11","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285711514358784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Notch Activation of Ca2+ 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