{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418519934290944.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/anie.201611659"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201611659"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201611659"}}],"dc:title":[{"@value":"Chemical Synthesis of Diubiquitin‐Based Photoaffinity Probes for Selectively Profiling Ubiquitin‐Binding Proteins"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Biochemical studies of cellular processes involving polyubiquitin have gained increasing attention. More tools are needed to identify ubiquitin (Ub)‐binding proteins. We report diazirine‐based photoaffinity probes that can capture Ub‐binding proteins in cell lysates, and show that diazirines are preferable to aryl azides as the photo‐crosslinking group, since they decrease non‐selective capture. Photoaffinity probes containing at least two Ub units were required to effectively capture Ub‐binding proteins. Different capture selectivity was observed for probes containing diubiquitin moieties with different types of linkages, thus indicating the potential to develop linkage‐dependent probes for selectively profiling Ub‐binding proteins under various cellular conditions.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418519934290948","@type":"Researcher","foaf:name":[{"@value":"Jun Liang"}],"jpcoar:affiliationName":[{"@value":"Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China  Hefei 230026 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519934290945","@type":"Researcher","foaf:name":[{"@value":"Lin Zhang"}],"jpcoar:affiliationName":[{"@value":"MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University  Beijing 100084 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1380579817369049345","@type":"Researcher","foaf:name":[{"@value":"Xiang‐Long Tan"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry Tsinghua University  Beijing 100084 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1380579817369049346","@type":"Researcher","foaf:name":[{"@value":"Yun‐Kun Qi"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry Tsinghua University  Beijing 100084 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519934290816","@type":"Researcher","foaf:name":[{"@value":"Shan Feng"}],"jpcoar:affiliationName":[{"@value":"MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University  Beijing 100084 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519934290947","@type":"Researcher","foaf:name":[{"@value":"Haiteng Deng"}],"jpcoar:affiliationName":[{"@value":"MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University  Beijing 100084 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519934290820","@type":"Researcher","foaf:name":[{"@value":"Yijing Yan"}],"jpcoar:affiliationName":[{"@value":"Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China  Hefei 230026 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1380579817369049347","@type":"Researcher","foaf:name":[{"@value":"Ji‐Shen Zheng"}],"jpcoar:affiliationName":[{"@value":"Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China  Hefei 230026 China"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418519934290817","@type":"Researcher","foaf:name":[{"@value":"Lei Liu"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry Tsinghua University  Beijing 100084 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Edition"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2017-02","prism:volume":"56","prism:number":"10","prism:startingPage":"2744","prism:endingPage":"2748"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201611659"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201611659"}],"createdAt":"2017-02-01","modifiedAt":"2023-09-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050017378354091776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Analysis of Selenoprotein F Binding to UDP-glucose:glycoprotein glucosyltransferase (UGGT) by a Photoreactive Crosslinker."},{"@value":"Analysis of Selenoprotein F Binding to UDP‐Glucose:Glycoprotein Glucosyltransferase (UGGT) by a Photoreactive 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