Transformation of maize with the <i>p1</i> transcription factor directs production of silk maysin, a corn earworm resistance factor, in concordance with a hierarchy of floral organ pigmentation

<jats:title>Summary</jats:title><jats:p>The maize <jats:italic>p1</jats:italic> gene encodes an R2R3‐MYB transcription factor that controls the biosynthesis of red flavonoid pigments in floral tissues of the maize plant. Genetic and quantitative trait locus analyses have also associated the <jats:italic>p1</jats:italic> gene with the synthesis of maysin, a flavone glycoside from maize silks that confers natural resistance to corn earworm. Here, we show directly that the <jats:italic>p1</jats:italic> gene induces maysin accumulation in silk tissues. Transformation of maize plants that had low or no silk maysin with <jats:italic>p1</jats:italic> transgenes elevated silk maysin concentrations to levels sufficient for corn earworm abiosis. The <jats:italic>p1</jats:italic> transgenes also conferred red pigment to pericarp, cob, husk and tassel tissues, as expected; however, different subsets of these tissues were pigmented within individual transgenic plants. Statistical analysis shows that the pigmentation patterns observed amongst the <jats:italic>p1</jats:italic> transgenic plants conform to a hierarchy that is similar to the temporal ordering of floral organ initiation. We propose that the observed hierarchy of pigmentation patterns is conferred by variation due to epigenetic control of the <jats:italic>p1</jats:italic> transgenes. The production of plants with improved traits through genetic engineering can depend in large part on the achievement of tight organ‐specific expression of the introduced transgenes. Our results demonstrate that the production of transgenic plants using a promoter with well‐defined tissue specificity, such as the <jats:italic>p1</jats:italic> promoter, can result in unexpected variation in tissue specificity amongst the resulting transgenic plants.</jats:p>