{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418520094899200.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0027-5107(99)00200-6"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0027510799002006?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0027510799002006?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"10751600"}}],"dc:title":[{"@value":"Age-associated increase of spontaneous mutant frequency and molecular nature of mutation in newborn and old lacZ-transgenic mouse"}],"description":[{"notation":[{"@value":"Accumulation of mutation has long been hypothesized to be a cause of aging and contribute to many of the degenerative diseases, which appear in the senescent phase of life. To test this hypothesis, age-associated changes in spontaneous mutation in different tissues of the body as well as the molecular nature of such changes should be examined. This kind of approach has become feasible only lately with a development of new transgenic mice suitable for mutation assay. Here, using one of these transgenic mice harboring lacZ gene, we have shown that the age-associated increase in spontaneous mutant frequency is common to all tissues examined; spleen, liver, heart, brain, skin and testis, while the rates of increase in mutant frequency differed among the tissues. DNA sequencing of the 496 lacZ mutants recovered from the tissues of newborn and old mice has revealed that spectra of mutations are similar at the two age points with G:C to A:T transition at CpG site being a predominant type of mutation. Furthermore, some mutations in old tissues are complex type and not found in tissues of newborn mice. These results suggest that similar mechanisms may be operating for mutation induction in fetal and postnatal aging process. In addition, the appearance of complex types of mutations in the old tissues suggests a unique cause for these mutations in aging tissues."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418520094899203","@type":"Researcher","foaf:name":[{"@value":"Tetsuya Ono"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899205","@type":"Researcher","foaf:name":[{"@value":"Hironobu Ikehata"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899206","@type":"Researcher","foaf:name":[{"@value":"Shingo Nakamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899207","@type":"Researcher","foaf:name":[{"@value":"Yusuke Saito"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899200","@type":"Researcher","foaf:name":[{"@value":"Yoshio Hosoi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899201","@type":"Researcher","foaf:name":[{"@value":"Yoshihiro Takai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899204","@type":"Researcher","foaf:name":[{"@value":"Shogo Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899208","@type":"Researcher","foaf:name":[{"@value":"Junichi Onodera"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520094899202","@type":"Researcher","foaf:name":[{"@value":"Kazuo Yamamoto"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00275107"}],"prism:publicationName":[{"@value":"Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2000-02","prism:volume":"447","prism:number":"2","prism:startingPage":"165","prism:endingPage":"177"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0027510799002006?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0027510799002006?httpAccept=text/plain"}],"createdAt":"2002-07-25","modifiedAt":"2020-02-04","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Aging","dc:title":"Aging"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Myocardium","dc:title":"Myocardium"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Mutational%20Analysis","dc:title":"DNA Mutational Analysis"},{"@id":"https://cir.nii.ac.jp/all?q=Brain","dc:title":"Brain"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Transgenic","dc:title":"Mice, Transgenic"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Animals,%20Newborn","dc:title":"Animals, Newborn"},{"@id":"https://cir.nii.ac.jp/all?q=Lac%20Operon","dc:title":"Lac Operon"},{"@id":"https://cir.nii.ac.jp/all?q=Liver","dc:title":"Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Testis","dc:title":"Testis"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=Spleen","dc:title":"Spleen"},{"@id":"https://cir.nii.ac.jp/all?q=Skin","dc:title":"Skin"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285707328572800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of calorie restriction on the age-dependent accumulation of mutations in the small intestine of lacZ-transgenic mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204239790848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Absence of Mutagenic Effects of 2.45 GHz Radiofrequency Exposure in Spleen, Liver, Brain, and Testis of lacZ-Transgenic Mouse Exposed in Utero"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205257945600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Maintenance of Genome Integrity is Tissue-Specific"},{"@language":"ja-Kana","@value":"Maintenance of Genome Integrity is Tissue Specific"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679220877696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Embryonic Lethality in Mice Lacking Mismatch-Specific Thymine DNA Glycosylase Is Partially Prevented by DOPS, a Precursor of Noradrenaline"}]},{"@id":"https://cir.nii.ac.jp/crid/2051433316888967936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various 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