{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418520353079552.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jvi.77.21.11781-11789.2003"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/JVI.77.21.11781-11789.2003"}}],"dc:title":[{"@value":"Active Borna Disease Virus Polymerase Complex Requires a Distinct Nucleoprotein-to-Phosphoprotein Ratio but No Viral X Protein"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>Analysis of the composition and regulation of the Borna disease virus (BDV) polymerase complex has so far been limited by the lack of a functional assay. To establish such an assay on the basis of an artificial minigenome, we constructed expression vectors encoding either nucleoprotein (N), phosphoprotein (P), X protein, or polymerase (L) of BDV under the control of the chicken β-actin promoter. A Flag-tagged version of L colocalized with virus-encoded N and P in characteristic nuclear dots of BDV-infected cells and increased viral N-protein levels in persistently infected Vero cells. Vector-driven expression of L, N, and P in BSR-T7 cells together with a negative-sense BDV minigenome carrying a chloramphenicol acetyltransferase (CAT) reporter gene resulted in efficient synthesis of CAT protein. Induction of CAT protein synthesis strongly depended on a 10- to 30-fold molar excess of the N-encoding plasmid over the P-encoding plasmid. Cotransfection of even small amounts of plasmid encoding the viral X protein reduced CAT synthesis to background levels. Thus, the N-to-P stoichiometry seems to play a central role in the regulation of the BDV polymerase complex. Our data further suggest a negative regulatory function for the X protein of BDV.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418520353079425","@type":"Researcher","foaf:name":[{"@value":"Urs Schneider"}],"jpcoar:affiliationName":[{"@value":"Department of Virology, University of Freiburg, D-79104 Freiburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520353079426","@type":"Researcher","foaf:name":[{"@value":"Melanie Naegele"}],"jpcoar:affiliationName":[{"@value":"Department of Virology, University of Freiburg, D-79104 Freiburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520353079552","@type":"Researcher","foaf:name":[{"@value":"Peter Staeheli"}],"jpcoar:affiliationName":[{"@value":"Department of Virology, University of Freiburg, D-79104 Freiburg, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520353079424","@type":"Researcher","foaf:name":[{"@value":"Martin Schwemmle"}],"jpcoar:affiliationName":[{"@value":"Department of Virology, University of Freiburg, D-79104 Freiburg, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0022538X"},{"@type":"EISSN","@value":"10985514"}],"prism:publicationName":[{"@value":"Journal of Virology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2003-11","prism:volume":"77","prism:number":"21","prism:startingPage":"11781","prism:endingPage":"11789"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/JVI.77.21.11781-11789.2003"}],"createdAt":"2003-10-13","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335796974464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Inhibition of Borna disease virus replication by an endogenous bornavirus-like element in the ground squirrel genome."}]},{"@id":"https://cir.nii.ac.jp/crid/1050001335813620224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Nucleocytoplasmic shuttling of viral proteins in borna disease virus infection."}]},{"@id":"https://cir.nii.ac.jp/crid/1050282810792905984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus."}]},{"@id":"https://cir.nii.ac.jp/crid/1050564285789146880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus."},{"@value":"An RNA-dependent RNA polymerase gene derived from ancient negative-strand RNA virus in bat genomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1050845760662195072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Bornavirus closely associates and segregates with host chromosomes to ensure persistent intranuclear infection."}]},{"@id":"https://cir.nii.ac.jp/crid/1050845760689262592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species."}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232551017984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Paleovirology of bornaviruses: What can be learned from molecular fossils of bornaviruses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643728258816","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Bornavirus infection in human diseases and its molecular neuropathology"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298757208004864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Reverse Genetics and Artificial Replication Systems of Borna Disease Virus 1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183936384512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Borna Disease Virus Assembles Porous Cage-like Viral Factories in the Nucleus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118724141696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Borna Disease Virus 2 (BoDV-2) Nucleoprotein Is a Conspecific Protein That Enhances BoDV-1 RNA-Dependent RNA Polymerase Activity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118724940288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Borna disease virus phosphoprotein triggers the organization of viral inclusion bodies by liquid-liquid phase separation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567816129920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Reverse genetics approaches of Borna disease virus: applications in development of viral vectors and preventive vaccines"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680055216768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"内在性RNA ウイルスエレメントによるウイルスRNA 制御仮説"},{"@language":"en","@value":"Possible roles of endogenous RNA virus elements in RNA virus infection"},{"@language":"ja-Kana","@value":"ナイザイセイ RNA ウイルスエレメント ニ ヨル ウイルス RNA セイギョ カセツ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jvi.77.21.11781-11789.2003"},{"@type":"CROSSREF","@value":"10.1073/pnas.1407046111_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0051161_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1111/cen3.12686_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.3390/v14102236_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1074/jbc.m116.746396_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.3390/v5081978_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1016/j.virusres.2018.04.006_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1128/jvi.00936-21_references_DOI_1horWGRX8ePGmES1DAWlOlQJIdT"},{"@type":"CROSSREF","@value":"10.1016/j.ijbiomac.2021.09.153_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1038/srep25873_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1111/1348-0421.12193_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1016/j.coviro.2020.05.011_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.2222/jsv.66.39_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"},{"@type":"CROSSREF","@value":"10.1016/j.chom.2012.04.009_references_DOI_4pVyj8vQywv3BFQSjlkRpkE9fDJ"}]}