{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418520509643136.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/gr.977903"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/gr.977903"}},{"identifier":{"@type":"NAID","@value":"30018929690"}}],"dc:title":[{"@value":"Large-Scale Discovery of Induced Point Mutations With High-Throughput TILLING"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>TILLING (<jats:underline>T</jats:underline>argeting <jats:underline>I</jats:underline>nduced <jats:underline>L</jats:underline>ocal<jats:underline>L</jats:underline>esions <jats:underline>in</jats:underline><jats:underline>G</jats:underline>enomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms. We have developed TILLING as a service to the <jats:italic>Arabidopsis</jats:italic> community known as the <jats:italic>Arabidopsis</jats:italic>TILLING Project (ATP). Our goal is to rapidly deliver allelic series of ethylmethanesulfonate-induced mutations in target 1-kb loci requested by the international research community. In the first year of public operation, ATP has discovered, sequenced, and delivered >1000 mutations in >100 genes ordered by <jats:italic>Arabidopsis</jats:italic> researchers. The tools and methodologies described here can be adapted to create similar facilities for other organisms.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418520509643139","@type":"Researcher","foaf:name":[{"@value":"Bradley J. Till"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643138","@type":"Researcher","foaf:name":[{"@value":"Steven H. Reynolds"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643136","@type":"Researcher","foaf:name":[{"@value":"Elizabeth A. Greene"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643143","@type":"Researcher","foaf:name":[{"@value":"Christine A. Codomo"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643148","@type":"Researcher","foaf:name":[{"@value":"Linda C. Enns"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643145","@type":"Researcher","foaf:name":[{"@value":"Jessica E. Johnson"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643142","@type":"Researcher","foaf:name":[{"@value":"Chris Burtner"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643140","@type":"Researcher","foaf:name":[{"@value":"Anthony R. Odden"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643144","@type":"Researcher","foaf:name":[{"@value":"Kim Young"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643141","@type":"Researcher","foaf:name":[{"@value":"Nicholas E. Taylor"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643137","@type":"Researcher","foaf:name":[{"@value":"Jorja G. Henikoff"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643146","@type":"Researcher","foaf:name":[{"@value":"Luca Comai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418520509643147","@type":"Researcher","foaf:name":[{"@value":"Steven Henikoff"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10889051"},{"@type":"EISSN","@value":"15495469"},{"@type":"NCID","@value":"AA11054029"}],"prism:publicationName":[{"@value":"Genome Research"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2003-03-01","prism:volume":"13","prism:number":"3","prism:startingPage":"524","prism:endingPage":"530"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/gr.977903"}],"createdAt":"2003-03-04","modifiedAt":"2021-11-18","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282677518724480","@type":"Article","resourceType":"学術雑誌論文(journal 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