{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361418521206561920.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1365-2656.2010.01722.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2656.2010.01722.x"}},{"identifier":{"@type":"URI","@value":"https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2656.2010.01722.x"}}],"dc:title":[{"@value":"Carbon isotope fractionation of amino acids in fish muscle reflects biosynthesis and isotopic routing from dietary protein"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p> <jats:bold>1.</jats:bold> Analysis of stable carbon isotopes is a valuable tool for studies of diet, habitat use and migration. However, significant variability in the degree of trophic fractionation (Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub>) between consumer (C) and diet (D) has highlighted our lack of understanding of the biochemical and physiological underpinnings of stable isotope ratios in tissues.</jats:p><jats:p> <jats:bold>2.</jats:bold> An opportunity now exists to increase the specificity of dietary studies by analyzing the δ<jats:sup>13</jats:sup>C values of amino acids (AAs). Common mummichogs (<jats:italic>Fundulus heteroclitus</jats:italic>, Linnaeus 1766) were reared on four isotopically distinct diets to examine individual AA Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> variability in fish muscle.</jats:p><jats:p> <jats:bold>3.</jats:bold> Modest bulk tissue Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> values reflected relatively large trophic fractionation for many non‐essential AAs and little to no fractionation for all essential AAs.</jats:p><jats:p> <jats:bold>4.</jats:bold> Essential AA δ<jats:sup>13</jats:sup>C values were not significantly different between diet and consumer (Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> = 0·0 ± 0·4‰), making them ideal tracers of carbon sources at the base of the food web. Stable isotope analysis of muscle essential AAs provides a promising tool for dietary reconstruction and identifying baseline δ<jats:sup>13</jats:sup>C values to track animal movement through isotopically distinct food webs.</jats:p><jats:p> <jats:bold>5.</jats:bold> Non‐essential AA Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> values showed evidence of both <jats:italic>de novo</jats:italic> biosynthesis and direct isotopic routing from dietary protein. We attributed patterns in Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> to variability in protein content and AA composition of the diet as well as differential utilization of dietary constituents contributing to the bulk carbon pool. This variability illustrates the complicated nature of metabolism and suggests caution must be taken with the assumptions used to interpret bulk stable isotope data in dietary studies.</jats:p><jats:p> <jats:bold>6.</jats:bold> Our study is the first to investigate the expression of AA Δ<jats:sup>13</jats:sup>C<jats:sub>C‐D</jats:sub> values for a marine vertebrate and should provide for significant refinements in studies of diet, habitat use and migration using stable isotopes.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381418521206561921","@type":"Researcher","foaf:name":[{"@value":"Kelton W. McMahon"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418521206561922","@type":"Researcher","foaf:name":[{"@value":"Marilyn L. Fogel"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418521206561923","@type":"Researcher","foaf:name":[{"@value":"Travis S. Elsdon"}]},{"@id":"https://cir.nii.ac.jp/crid/1381418521206561920","@type":"Researcher","foaf:name":[{"@value":"Simon R. Thorrold"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00218790"},{"@type":"EISSN","@value":"13652656"}],"prism:publicationName":[{"@value":"Journal of Animal Ecology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2010-08-05","prism:volume":"79","prism:number":"5","prism:startingPage":"1132","prism:endingPage":"1141"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2656.2010.01722.x"},{"@id":"https://besjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2656.2010.01722.x"}],"createdAt":"2010-07-09","modifiedAt":"2023-11-02","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142672968192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A method for stable carbon isotope measurement of underivatized individual amino acids by multi‐dimensional high‐performance liquid chromatography and elemental analyzer/isotope ratio mass spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142732286976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Theoretical Amino Acid-Specific Radiocarbon Content in the Environment: Hypotheses to Be Tested and Opportunities to Be Taken"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283689510382080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intra‐trophic isotopic discrimination of <sup>15</sup>N/<sup>14</sup>N for amino acids in autotrophs: Implications for nitrogen dynamics in ecological studies"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846639463553920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"High‐resolution food webs based on nitrogen isotopic composition of amino acids"}]},{"@id":"https://cir.nii.ac.jp/crid/2050307417121637504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A new insight into isotopic fractionation associated with decarboxylation in organisms : implications for amino acid isotope approaches in biogeoscience"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1365-2656.2010.01722.x"},{"@type":"CROSSREF","@value":"10.1002/rcm.8885_references_DOI_V41BLKMwcBWU0iPUVzSMulLp7io"},{"@type":"CROSSREF","@value":"10.3389/fmars.2020.00302_references_DOI_V41BLKMwcBWU0iPUVzSMulLp7io"},{"@type":"CROSSREF","@value":"10.1002/ece3.2866_references_DOI_V41BLKMwcBWU0iPUVzSMulLp7io"},{"@type":"CROSSREF","@value":"10.1186/s40645-020-00364-w_references_DOI_V41BLKMwcBWU0iPUVzSMulLp7io"},{"@type":"CROSSREF","@value":"10.1002/ece3.1103_references_DOI_V41BLKMwcBWU0iPUVzSMulLp7io"}]}