{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361694366532007168.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.bbrc.2019.10.174"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X19320844?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X19320844?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"31708103"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Mechanotransduction via the Piezo1-Akt pathway underlies Sost suppression in osteocytes"}],"description":[{"notation":[{"@value":"Osteocytes function as critical regulators of bone homeostasis by coordinating the functions of osteoblasts and osteoclasts, and are constantly exposed to mechanical force. However, the molecular mechanism underlying the mechanical signal transduction in osteocytes is not well understood. Here, we found that Yoda1, a selective Piezo1 agonist, increased intracellular calcium mobilization and dose-dependently decreased the expression of Sost (encoding Sclerostin) in the osteocytic cell line IDG-SW3. We also demonstrated that mechanical stretch of IDG-SW3 suppressed Sost expression, a result which was abrogated by treatment with the Piezo1 inhibitor GsMTx4, and the deficiency of Piezo1. Furthermore, the suppression of Sost expression was abolished by treatment with an Akt inhibitor. Taken together, these results indicate that the activation of the Piezo1-Akt pathway in osteocytes is required for mechanical stretch-induced downregulation of Sost expression."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381694366532007171","@type":"Researcher","foaf:name":[{"@value":"Fumiyuki Sasaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1381694366532007298","@type":"Researcher","foaf:name":[{"@value":"Mikihito Hayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381694366532007052","@type":"Researcher","foaf:name":[{"@value":"Yuki Mouri"}]},{"@id":"https://cir.nii.ac.jp/crid/1381694366532007045","@type":"Researcher","foaf:name":[{"@value":"Satoshi Nakamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1381694366532007177","@type":"Researcher","foaf:name":[{"@value":"Taiji Adachi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381694366532007176","@type":"Researcher","foaf:name":[{"@value":"Tomoki Nakashima"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006291X"}],"prism:publicationName":[{"@value":"Biochemical and Biophysical Research Communications"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2020-01","prism:volume":"521","prism:number":"3","prism:startingPage":"806","prism:endingPage":"813"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0006291X19320844?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0006291X19320844?httpAccept=text/plain"}],"createdAt":"2019-11-08","modifiedAt":"2025-09-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Down-Regulation","dc:title":"Down-Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Mechanotransduction,%20Cellular","dc:title":"Mechanotransduction, Cellular"},{"@id":"https://cir.nii.ac.jp/all?q=Osteocytes","dc:title":"Osteocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Ion%20Channels","dc:title":"Ion Channels"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Proto-Oncogene%20Proteins%20c-akt","dc:title":"Proto-Oncogene Proteins c-akt"},{"@id":"https://cir.nii.ac.jp/all?q=Adaptor%20Proteins,%20Signal%20Transducing","dc:title":"Adaptor Proteins, Signal Transducing"},{"@id":"https://cir.nii.ac.jp/all?q=Signal%20Transduction","dc:title":"Signal Transduction"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782023762048","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19K18452"},{"@type":"JGN","@value":"JP19K18452"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K18452/"}],"notation":[{"@language":"ja","@value":"新規スクリーニング法を用いた骨細胞特異的なメカニカルストレス受容体の同定"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050002212336090240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"TRPV2 is critical for the maintenance of cardiac structure and function in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1050011307246246656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering 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