{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993406623360.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1074/jbc.m309057200"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820755523?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820755523?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1074/jbc.M309057200"}},{"identifier":{"@type":"PMID","@value":"14522963"}}],"dc:title":[{"@value":"Formation of Acrolein-derived 2′-Deoxyadenosine Adduct in an Iron-induced Carcinogenesis Model"}],"description":[{"notation":[{"@value":"Acrolein is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidase-catalyzed amino acid oxidation. It shows facile reactivity toward DNA to form an exocyclic DNA adduct. To verify the formation of acrolein-derived DNA adduct under oxidative stress in vivo, we raised a novel monoclonal antibody (mAb21) against the acrolein-modified DNA and found that the antibody most significantly recognized an acrolein-modified 2' -deoxyadenosine. On the basis of chemical and spectroscopic evidence, the major antigenic product of mAb21 was the 1,N6-propano-2' -deoxyadenosine adduct. The exposure of rat liver epithelial RL34 cells to acrolein resulted in a significant accumulation of the acrolein-2' -deoxyadenosine adduct in the nuclei. Formation of this adduct under oxidative stress in vivo was immunohistochemically examined in rats exposed to ferric nitrilotriacetate, a carcinogenic iron chelate that specifically induces oxidative stress in the kidneys of rodents. It was observed that the acrolein-2' -deoxyadenosine adduct was formed in the nuclei of the proximal tubular cells, the target cells of this carcinogenesis model. The same cells were stained with a monoclonal antibody 5F6 that recognizes an acrolein-lysine adduct, by which cytosolic accumulation of acrolein-modified proteins appeared. Similar results were also obtained from myeloperoxidase knockout mice exposed to the iron complex, suggesting that the myeloperoxidase-catalyzed oxidation system might not be essential for the generation of acrolein in this experimental animal carcinogenesis model. The data obtained in this study suggest that the formation of a carcinogenic aldehyde through lipid peroxidation may be causally involved in the pathophysiological effects associated with oxidative stress."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993406623360","@type":"Researcher","foaf:name":[{"@value":"Yoshichika Kawai"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993406623490","@type":"Researcher","foaf:name":[{"@value":"Atsunori Furuhata"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993406623361","@type":"Researcher","foaf:name":[{"@value":"Shinya Toyokuni"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801206887424","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30192470"},{"@type":"NRID","@value":"1000030192470"},{"@type":"NRID","@value":"9000253179859"},{"@type":"NRID","@value":"9000018815715"},{"@type":"NRID","@value":"9000318579478"},{"@type":"NRID","@value":"9000347321513"},{"@type":"NRID","@value":"9000021027081"},{"@type":"NRID","@value":"9000014161643"},{"@type":"NRID","@value":"9000238241897"},{"@type":"NRID","@value":"9000253204758"},{"@type":"NRID","@value":"9000391621534"},{"@type":"NRID","@value":"9000391781456"},{"@type":"NRID","@value":"9000004415522"},{"@type":"NRID","@value":"9000021006091"},{"@type":"NRID","@value":"9000010687097"},{"@type":"NRID","@value":"9000010412574"},{"@type":"NRID","@value":"9000000360966"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/y-aratani"}],"foaf:name":[{"@value":"Yasuaki Aratani"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993406623489","@type":"Researcher","foaf:name":[{"@value":"Koji Uchida"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219258"}],"prism:publicationName":[{"@value":"Journal of Biological Chemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2003-12","prism:volume":"278","prism:number":"50","prism:startingPage":"50346","prism:endingPage":"50354"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0021925820755523?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0021925820755523?httpAccept=text/plain"},{"@id":"https://syndication.highwire.org/content/doi/10.1074/jbc.M309057200"}],"createdAt":"2003-12-05","modifiedAt":"2023-04-27","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Threonine","dc:title":"Threonine"},{"@id":"https://cir.nii.ac.jp/all?q=Time%20Factors","dc:title":"Time Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Iron","dc:title":"Iron"},{"@id":"https://cir.nii.ac.jp/all?q=Enzyme-Linked%20Immunosorbent%20Assay","dc:title":"Enzyme-Linked Immunosorbent Assay"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Transgenic","dc:title":"Mice, Transgenic"},{"@id":"https://cir.nii.ac.jp/all?q=Thymus%20Gland","dc:title":"Thymus Gland"},{"@id":"https://cir.nii.ac.jp/all?q=Binding,%20Competitive","dc:title":"Binding, Competitive"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Adducts","dc:title":"DNA Adducts"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Neoplasms","dc:title":"Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Acrolein","dc:title":"Acrolein"},{"@id":"https://cir.nii.ac.jp/all?q=Rats,%20Wistar","dc:title":"Rats, Wistar"},{"@id":"https://cir.nii.ac.jp/all?q=Peroxidase","dc:title":"Peroxidase"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Nucleus","dc:title":"Cell Nucleus"},{"@id":"https://cir.nii.ac.jp/all?q=Ions","dc:title":"Ions"},{"@id":"https://cir.nii.ac.jp/all?q=Aldehydes","dc:title":"Aldehydes"},{"@id":"https://cir.nii.ac.jp/all?q=Deoxyadenosines","dc:title":"Deoxyadenosines"},{"@id":"https://cir.nii.ac.jp/all?q=Dose-Response%20Relationship,%20Drug","dc:title":"Dose-Response Relationship, Drug"},{"@id":"https://cir.nii.ac.jp/all?q=Lysine","dc:title":"Lysine"},{"@id":"https://cir.nii.ac.jp/all?q=Antibodies,%20Monoclonal","dc:title":"Antibodies, Monoclonal"},{"@id":"https://cir.nii.ac.jp/all?q=Immunohistochemistry","dc:title":"Immunohistochemistry"},{"@id":"https://cir.nii.ac.jp/all?q=Rats","dc:title":"Rats"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Oxygen","dc:title":"Oxygen"},{"@id":"https://cir.nii.ac.jp/all?q=Oxidative%20Stress","dc:title":"Oxidative Stress"},{"@id":"https://cir.nii.ac.jp/all?q=Liver","dc:title":"Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Models,%20Chemical","dc:title":"Models, Chemical"},{"@id":"https://cir.nii.ac.jp/all?q=Cattle","dc:title":"Cattle"},{"@id":"https://cir.nii.ac.jp/all?q=Lipid%20Peroxidation","dc:title":"Lipid Peroxidation"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282813782579200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Iron and thiol redox signaling in cancer: An exquisite balance to escape ferroptosis"},{"@value":"Iron and thiol redox signaling in cancer: An exquisite balance ot escape ferroptosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232015369728","@type":"Article","resourceType":"学術雑誌論文(journal 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