{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993546948736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.jviromet.2009.10.026"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0166093409004650?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0166093409004650?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"19883692"}}],"dc:title":[{"@value":"A simple assay system for infection-enhancing and -neutralizing antibodies to dengue type 2 virus using layers of semi-adherent K562 cells"}],"description":[{"notation":[{"@value":"A simple alternative to the dengue antibody-dependent enhancement (ADE) assay was established. The new assay method utilizes cells attached to microplate wells, thereby eliminating cumbersome procedures typical of the conventional ADE assay that utilizes suspension cells. Semi-adherent K562 cells bearing the Fc-gamma receptor (Fc gammaR) were cultured on poly-L-lysine-coated plates. The procedure consisted of (i) preparation of a virus-antibody-cell mixture in wells, (ii) cultivation at 37 degrees C for 24 h and (iii) fixation and immunostaining to count infected cells. Using monoclonal antibodies against dengue type 2 virus, the new system correlated with three conventional systems. Additionally, K562 cells were employed in a neutralization test. For this purpose, the virus-antibody mixture was incubated at 37 degrees C for 2 h prior to the addition of cells. As expected, K562 cells provided lower neutralizing antibody titers than did a conventional neutralization test using Vero cells, which do not have Fc gammaR, in monoclonals showing both neutralizing and enhancing activities. Since antibodies are present in polyclonal form in circulation, neutralization tests using K562 cells are considered to reveal a more accurate in vivo status than those using Vero cells. Human sera, positive for dengue virus antibodies, showed neutralizing and enhancing activities in a dose-dependent manner."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993546948736","@type":"Researcher","foaf:name":[{"@value":"Eiji Konishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993546948737","@type":"Researcher","foaf:name":[{"@value":"Yuko Tabuchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993546948738","@type":"Researcher","foaf:name":[{"@value":"Atsushi Yamanaka"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01660934"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/01660934"}],"prism:publicationName":[{"@value":"Journal of Virological Methods"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2010-02","prism:volume":"163","prism:number":"2","prism:startingPage":"360","prism:endingPage":"367"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0166093409004650?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0166093409004650?httpAccept=text/plain"}],"createdAt":"2009-11-04","modifiedAt":"2025-09-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Immunoassay","dc:title":"Immunoassay"},{"@id":"https://cir.nii.ac.jp/all?q=Dengue%20Virus","dc:title":"Dengue Virus"},{"@id":"https://cir.nii.ac.jp/all?q=Antibodies,%20Viral","dc:title":"Antibodies, Viral"},{"@id":"https://cir.nii.ac.jp/all?q=Antibodies,%20Neutralizing","dc:title":"Antibodies, Neutralizing"},{"@id":"https://cir.nii.ac.jp/all?q=Antibody-Dependent%20Enhancement","dc:title":"Antibody-Dependent Enhancement"},{"@id":"https://cir.nii.ac.jp/all?q=Sensitivity%20and%20Specificity","dc:title":"Sensitivity and Specificity"},{"@id":"https://cir.nii.ac.jp/all?q=Dengue","dc:title":"Dengue"},{"@id":"https://cir.nii.ac.jp/all?q=Neutralization%20Tests","dc:title":"Neutralization Tests"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=K562%20Cells","dc:title":"K562 Cells"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050287297252289792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Japanese encephalitis vaccine-facilitated dengue virus infection-enhancement antibody in adults"}]},{"@id":"https://cir.nii.ac.jp/crid/1050568772240586240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Human antibody response to dengue virus: implications for dengue vaccine design"}]},{"@id":"https://cir.nii.ac.jp/crid/1050575520349052032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Japanese Encephalitis DNA Vaccines with Epitope Modification Reduce the Induction of Cross-Reactive Antibodies against Dengue Virus and Antibody-Dependent Enhancement of Dengue Virus Infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1050856995321770880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An affinity-matured human monoclonal antibody targeting fusion loop epitope of dengue virus with in vivo therapeutic potency"},{"@value":"An affinity-matured human monoclonal antibody targeting fusion loop epitope of dengue virus with\n                  <i>in vivo</i>\n                  therapeutic potency"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002219389209088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dengue Virus Infection-Enhancing Activity in Serum Samples with Neutralizing Activity as Determined by Using FcγR-Expressing Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711774900480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dengue transmission model by means of viremic adult immuno-competent mouse"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184719785472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dengue virus infection-enhancing activity of undiluted sera obtained from patients with secondary dengue virus infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660376348288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection‐enhancing antibody response"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205078939776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Research Activities in Kobe-Indonesia Collaborative Research Centers"},{"@language":"ja","@value":"神戸大学インドネシア拠点における国際共同研究活動"},{"@language":"ja-Kana","@value":"コウベ ダイガク インドネシア キョテン ニ オケル コクサイ キョウドウ ケンキュウ カツドウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001288151350016","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Indonesia-Kobe University Collaborative Research Center for Emerging and Reemerging Infectious Diseases (CRC-ERID) J-GRID (Japan Initiative for Global Research Network on Infectious Diseases)"}]},{"@id":"https://cir.nii.ac.jp/crid/1390019204223328896","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Research on mosquito-borne pathogens, especially mosquito susceptibility and its vector competence for viruses"},{"@language":"ja","@value":"蚊が媒介する病原体，特に蚊のウイルス感受性と媒介能に関する研究"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680195955072","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Issues Related to Recent Dengue Vaccine Development"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681218866944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Dengue-Immune Humans Have Higher Levels of Complement-Independent Enhancing Antibody than Complement-Dependent Neutralizing Antibody"}]},{"@id":"https://cir.nii.ac.jp/crid/1390565134838622848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Human Heavy Chain Antibody Genes Elicited in Thai Dengue Patients during DENV2 Secondary Infection"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.jviromet.2009.10.026"},{"@type":"OPENAIRE","@value":"doi_dedup___::abe65dfa583a069dec280941c4b90f01"},{"@type":"CROSSREF","@value":"10.1371/journal.pntd.0001536_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.20965/jdr.2014.p0828_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1186/s41182-016-0004-y_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1101/2020.10.03.324731_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1186/1756-3305-7-143_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.7883/yoken.jjid.2016.379_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.7883/yoken.jjid.2019.235_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1038/s41598-021-92403-9_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.7601/mez.75.33_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1093/trstmh/trs007_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.2222/jsv.63.59_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.3390/vaccines10091411_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.2149/tmh.2011-s01_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1111/1348-0421.12125_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"},{"@type":"CROSSREF","@value":"10.1186/s12879-016-1873-8_references_DOI_QkKbd7X6Oy41MzDS8KvBB70m1Ve"}]}