{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993551384448.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/bc2003378"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/bc2003378"}},{"identifier":{"@type":"PMID","@value":"21838319"}}],"dc:title":[{"@value":"Zinc-Chelated Poly(1-vinylimidazole) and a Carbohydrate Ligand Polycation Form DNA Ternary Complexes for Gene Delivery"}],"description":[{"notation":[{"@value":"Zinc-chelated poly(1-vinylimidazole) (PVIm-Zn) and a carbohydrate ligand polycation, a poly(l-lysine) conjugated with lactose molecules (PLL-Lac), have formed DNA ternary complexes for gene delivery. The particle size of the PVIm-Zn/DNA complexes with negative zeta potential was decreased by the addition of the PLL-Lac. The resulting PLL-Lac/PVIm-Zn/DNA ternary complexes, which exhibited the pH-dependent dissociation of the PLL-Lac, mediated more gene expression than the PVIm/DNA binary complexes. The PLL-Lac/PVIm-Zn/DNA complexes with the specific recognition of cell surface receptors mediated the highest gene expression without cytotoxicity at a relatively lower charge ratio (positive/negative = 2.5). These results suggest that the pH-dependent dissociation of the carbohydrate ligands after the recognition of cell surface receptors, including the physicochemical and biochemical function of PVIm-Zn, played an important role in gene expression."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993551384450","@type":"Researcher","foaf:name":[{"@value":"Shoichiro Asayama"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993551384448","@type":"Researcher","foaf:name":[{"@value":"Satoshi Nishinohara"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993551384449","@type":"Researcher","foaf:name":[{"@value":"Hiroyoshi Kawakami"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10431802"},{"@type":"EISSN","@value":"15204812"}],"prism:publicationName":[{"@value":"Bioconjugate Chemistry"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2011-08-25","prism:volume":"22","prism:number":"9","prism:startingPage":"1864","prism:endingPage":"1868"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/bc2003378"}],"createdAt":"2011-08-15","modifiedAt":"2023-04-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Survival","dc:title":"Cell Survival"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Transfer%20Techniques","dc:title":"Gene Transfer Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Imidazoles","dc:title":"Imidazoles"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression","dc:title":"Gene Expression"},{"@id":"https://cir.nii.ac.jp/all?q=Antineoplastic%20Agents","dc:title":"Antineoplastic Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Lactose","dc:title":"Lactose"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen-Ion%20Concentration","dc:title":"Hydrogen-Ion Concentration"},{"@id":"https://cir.nii.ac.jp/all?q=Ligands","dc:title":"Ligands"},{"@id":"https://cir.nii.ac.jp/all?q=Polyelectrolytes","dc:title":"Polyelectrolytes"},{"@id":"https://cir.nii.ac.jp/all?q=Zinc","dc:title":"Zinc"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Polyamines","dc:title":"Polyamines"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Polylysine","dc:title":"Polylysine"},{"@id":"https://cir.nii.ac.jp/all?q=Polyvinyls","dc:title":"Polyvinyls"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Chelating%20Agents","dc:title":"Chelating Agents"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004230247219712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tuning of the methylimidazolium/imidazole balance in polycations for gene carrier"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691858316928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracellular co-delivery of zinc ions and plasmid DNA 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