{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993629705472.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/iai.68.7.4217-4224.2000"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/IAI.68.7.4217-4224.2000"}}],"dc:title":[{"@value":"The Most Abundant Glycoprotein of Amebic Cyst Walls (Jacob) Is a Lectin with Five Cys-Rich, Chitin-Binding Domains"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The infectious stage of amebae is the chitin-walled cyst, which is resistant to stomach acids. In this study an extraordinarily abundant, encystation-specific glycoprotein (Jacob) was identified on two-dimensional protein gels of cyst walls purified from\n            <jats:italic>Entamoeba invadens</jats:italic>\n            . Jacob, which was acidic and had an apparent molecular mass of ∼100 kDa, contained sugars that bound to concanavalin A and ricin. The\n            <jats:italic>jacob</jats:italic>\n            gene encoded a 45-kDa protein with a ladder-like series of five Cys-rich domains. These Cys-rich domains were reminiscent of but not homologous to the Cys-rich chitin-binding domains of insect chitinases and peritrophic matrix proteins that surround the food bolus in the insect gut. Jacob bound purified chitin and chitin remaining in sodium dodecyl sulfate-treated cyst walls. Conversely, the\n            <jats:italic>E. histolytica</jats:italic>\n            plasma membrane Gal/GalNAc lectin bound sugars of intact cyst walls and purified Jacob. In the presence of galactose,\n            <jats:italic>E. invadens</jats:italic>\n            formed wall-less cysts, which were quadranucleate and contained Jacob and chitinase (another encystation-specific protein) in secretory vesicles. A galactose lectin was found to be present on the surface of wall-less cysts, which phagocytosed bacteria and mucin-coated beads. These results suggest that the\n            <jats:italic>E. invadens</jats:italic>\n            cyst wall forms when the plasma membrane galactose lectin binds sugars on Jacob, which in turn binds chitin via its five chitin-binding domains.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993629705473","@type":"Researcher","foaf:name":[{"@value":"Marta Frisardi"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Immunology and Infectious Diseases1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705344","@type":"Researcher","foaf:name":[{"@value":"Sudip K. Ghosh"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Immunology and Infectious Diseases1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705474","@type":"Researcher","foaf:name":[{"@value":"Jessica Field"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Immunology and Infectious Diseases1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705476","@type":"Researcher","foaf:name":[{"@value":"Katrina Van Dellen"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Immunology and Infectious Diseases1 and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705345","@type":"Researcher","foaf:name":[{"@value":"Rick Rogers"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 2-->BioMedical Imaging Institute,2 Harvard School of Public Health, and"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705477","@type":"Researcher","foaf:name":[{"@value":"Phillips Robbins"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 3-->Department of Cell Biology, Boston University School of Dental Medicine,3 Boston, Massachusetts"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993629705472","@type":"Researcher","foaf:name":[{"@value":"John Samuelson"}],"jpcoar:affiliationName":[{"@value":"<!--label omitted: 1-->Department of Immunology and Infectious Diseases1 and"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1381699993629705475","@type":"Researcher","foaf:name":[{"@value":"W. A. Petri"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00199567"},{"@type":"EISSN","@value":"10985522"}],"prism:publicationName":[{"@value":"Infection and Immunity"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2000-07","prism:volume":"68","prism:number":"7","prism:startingPage":"4217","prism:endingPage":"4224"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/IAI.68.7.4217-4224.2000"}],"createdAt":"2002-07-27","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050287297252288640","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Entamoeba Encystation: New Targets to Prevent the Transmission of Amebiasis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017282431529472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"<i>Entamoeba</i>\n            Chitinase is Required for Mature Round Cyst Formation"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/iai.68.7.4217-4224.2000"},{"@type":"CROSSREF","@value":"10.1128/spectrum.00511-21_references_DOI_11iJCUoeEMxkvtaeWHNkFsBC86Y"},{"@type":"CROSSREF","@value":"10.1371/journal.ppat.1005845_references_DOI_11iJCUoeEMxkvtaeWHNkFsBC86Y"}]}