{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993663766144.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jb.184.10.2576-2586.2002"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/JB.184.10.2576-2586.2002"}}],"dc:title":[{"@value":"Advancing the Quorum in\n            <i>Pseudomonas aeruginosa</i>\n            : MvaT and the Regulation of\n            <i>N</i>\n            -Acylhomoserine Lactone Production and Virulence Gene Expression"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas aeruginosa</jats:italic>\n            regulates the production of many exoproteins and secondary metabolites via a hierarchical quorum-sensing cascade through LasR and RhlR and their cognate signal molecules\n            <jats:italic>N-</jats:italic>\n            (3-oxododecanoyl)-\n            <jats:sc>l</jats:sc>\n            -homoserine lactone (3O-C12-HSL) and\n            <jats:italic>N-</jats:italic>\n            (butanoyl)-\n            <jats:sc>l</jats:sc>\n            -homoserine lactone (C4-HSL). In this study, we found that transcription of the quorum sensing-regulated genes\n            <jats:italic>lecA</jats:italic>\n            (coding for PA-IL lectin),\n            <jats:italic>lasB</jats:italic>\n            (coding for elastase), and\n            <jats:italic>rpoS</jats:italic>\n            appeared to be growth phase dependent and their expression could not be advanced to the logarithmic phase in cells growing in batch culture by the addition of exogenous C4-HSL and 3O-C12-HSL. To identify novel regulators responsible for this growth phase dependency, a\n            <jats:italic>P. aeruginosa lecA</jats:italic>\n            ::\n            <jats:italic>lux</jats:italic>\n            reporter strain was subjected to random transposon mutagenesis. A number of mutants affected in\n            <jats:italic>lecA</jats:italic>\n            expression were found that exhibited altered production of multiple quorum sensing-dependent phenotypes. While some mutations were mapped to new loci such as\n            <jats:italic>clpA</jats:italic>\n            and\n            <jats:italic>mvaT</jats:italic>\n            and a putative efflux system, a number of mutations were also mapped to known regulators such as\n            <jats:italic>lasR</jats:italic>\n            ,\n            <jats:italic>rhlR</jats:italic>\n            , and\n            <jats:italic>rpoS.</jats:italic>\n            MvaT was identified as a novel global regulator of virulence gene expression, as a mutation in\n            <jats:italic>mvaT</jats:italic>\n            resulted in enhanced\n            <jats:italic>lecA</jats:italic>\n            expression and pyocyanin production. This mutant also showed altered swarming ability and production of the LasB and LasA proteases, 3O-C12-HSL, and C4-HSL. Furthermore, addition of exogenous 3O-C12-HSL and C4-HSL to the\n            <jats:italic>mvaT</jats:italic>\n            mutant significantly advanced\n            <jats:italic>lecA</jats:italic>\n            expression, suggesting that MvaT is involved in the growth phase-dependent regulation of the\n            <jats:italic>lecA</jats:italic>\n            gene.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993663766144","@type":"Researcher","foaf:name":[{"@value":"Stephen P. Diggle"}],"jpcoar:affiliationName":[{"@value":"School of Pharmaceutical Sciences"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993663766145","@type":"Researcher","foaf:name":[{"@value":"Klaus Winzer"}],"jpcoar:affiliationName":[{"@value":"Institute of Infections and Immunity, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993663766147","@type":"Researcher","foaf:name":[{"@value":"Andrée Lazdunski"}],"jpcoar:affiliationName":[{"@value":"Laboratoire d’Ingénierie des Systèmes Moléculaires, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993663766146","@type":"Researcher","foaf:name":[{"@value":"Paul Williams"}],"jpcoar:affiliationName":[{"@value":"School of Pharmaceutical Sciences"},{"@value":"Institute of Infections and Immunity, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993663766148","@type":"Researcher","foaf:name":[{"@value":"Miguel Cámara"}],"jpcoar:affiliationName":[{"@value":"School of Pharmaceutical Sciences"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219193"},{"@type":"EISSN","@value":"10985530"}],"prism:publicationName":[{"@value":"Journal of Bacteriology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2002-05-15","prism:volume":"184","prism:number":"10","prism:startingPage":"2576","prism:endingPage":"2586"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/JB.184.10.2576-2586.2002"}],"createdAt":"2002-07-27","modifiedAt":"2021-07-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360283689617116672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Ser/Thr/Tyr phosphoproteome analysis of pathogenic and non‐pathogenic <i>Pseudomonas</i> species"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691099817984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Nucleoid-associated proteins encoded on plasmids: Occurrence and mode of function"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jb.184.10.2576-2586.2002"},{"@type":"CROSSREF","@value":"10.1002/pmic.200800655_references_DOI_ZwFMR88G76o1f9SOu35P5JElXSS"},{"@type":"CROSSREF","@value":"10.1016/j.plasmid.2015.04.008_references_DOI_ZwFMR88G76o1f9SOu35P5JElXSS"}]}