{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993693558656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0168-9452(01)00496-4"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0168945201004964?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0168945201004964?httpAccept=text/plain"}}],"dc:title":[{"@value":"Agrobacterium-mediated transformation of Saintpaulia ionantha Wendl."}],"description":[{"notation":[{"@value":"Abstract   Agrobacterium tumefaciens strain LBA4404 that has pTOK233 and other two strains that have pIG121Hm were co-cultivated with suspension cells of Saintpaulia ionantha Wendl. ‘Pink Veil’. These two plasmids contain an intron-gusA reporter gene, hygromycin phosphotransferase gene (hpt) and neomycin phosphotransferase II gene (nptII) as selective markers. After 48 h of co-cultivation, the cells inoculated with A. tumefaciens strain LBA4404 (pTOK233) showed the highest GUS activity. After 4 months of subculture on the selection medium supplemented with 50 mg l−1 hygromycin B, resistant calli appeared and adventitious shoots were regenerated 6 months after the transfer on B5 medium supplemented with 1 mg l−1 BA. The presence of the gusA gene in the genome DNA of regenerated plants was detected by PCR and genomic Southern blot analysis. Leaf lamina, another explant used for inoculation, rarely expressed transient GUS activity, and transgenic plants were not obtained."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381699993693558784","@type":"Researcher","foaf:name":[{"@value":"Satoshi Kushikawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993693558785","@type":"Researcher","foaf:name":[{"@value":"Yoichiro Hoshino"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993693558656","@type":"Researcher","foaf:name":[{"@value":"Masahiro Mii"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01689452"}],"prism:publicationName":[{"@value":"Plant Science"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2001-10","prism:volume":"161","prism:number":"5","prism:startingPage":"953","prism:endingPage":"960"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0168945201004964?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0168945201004964?httpAccept=text/plain"}],"createdAt":"2002-07-25","modifiedAt":"2020-01-12","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Science","dc:title":"Plant Science"},{"@id":"https://cir.nii.ac.jp/all?q=Agronomy%20and%20Crop%20Science","dc:title":"Agronomy and Crop Science"},{"@id":"https://cir.nii.ac.jp/all?q=Genetics","dc:title":"Genetics"},{"@id":"https://cir.nii.ac.jp/all?q=General%20Medicine","dc:title":"General Medicine"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360005516124485504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298345423591680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The first genome for the Cape Primrose <scp><i>Streptocarpus rexii</i></scp> (Gesneriaceae), a model plant for studying meristem‐driven shoot diversity"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679305417216","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Pre-Culture Treatment Enhances Transient GUS Gene Expression in Leaf Segment of Saintpaulia ionantha Wendl. after Inoculation with Agrobacterium tumefaciens."},{"@value":"Pre-culture treatment enhances transient GUS gene expression on leaf segment of Saintpaulia ionantha Wendl. after inoculation with Agrobacterium tumefaciens"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/s0168-9452(01)00496-4"},{"@type":"OPENAIRE","@value":"doi_dedup___::fecca7512bdc564fe8dce275c9b24b76"},{"@type":"CROSSREF","@value":"10.1007/s12033-020-00248-w_references_DOI_CwxnRghzusVa1LodsZIuGZudjy7"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.19.149_references_DOI_CwxnRghzusVa1LodsZIuGZudjy7"},{"@type":"CROSSREF","@value":"10.1002/pld3.388_references_DOI_CwxnRghzusVa1LodsZIuGZudjy7"}]}