{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993769684352.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1093/pcp/pcn132"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/pcp/article-pdf/49/10/1600/5345875/pcn132.pdf"}},{"identifier":{"@type":"PMID","@value":"18772185"}}],"dc:title":[{"@value":"Crucial Role in Light Signal Transduction for the Conserved Met93 of the BLUF Protein PixD/Slr1694"}],"description":[{"notation":[{"@value":"PixD/Slr1694 from the cyanobacterium Synechocystis sp. PCC6803 is a member of a new class of flavin-containing blue-light sensory proteins containing a BLUF (blue light using flavin) domain. The photocycle reaction mechanism of BLUF is unique because only small structural changes of a bound chromophore are accompanied by a few hydrogen bond rearrangements in the chromophore-binding site. Here, we show that in PixD, Met93, the residue conserved in all BLUF domains, is crucial for light-dependent signal transduction. Specifically, the light-insensitive M93A mutant of PixD revealed biochemical and physiological activities compatible with those of the light-adapted wild-type PixD. However, the W91A mutant of PixD retained light sensitivity and biological function, although the corresponding mutant of another BLUF protein, AppA, has been reported to be locked in the light signaling state. These observations suggest that the pathway through which the light signal is transformed into apoprotein structural changes has been modified in BLUF proteins for their respective functions."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380285706518551937","@type":"Researcher","foaf:name":[{"@value":"S. Masuda"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993769684355","@type":"Researcher","foaf:name":[{"@value":"K. Hasegawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993769684352","@type":"Researcher","foaf:name":[{"@value":"H. Ohta"}]},{"@id":"https://cir.nii.ac.jp/crid/1381699993769684353","@type":"Researcher","foaf:name":[{"@value":"T.-a. Ono"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00320781"},{"@type":"EISSN","@value":"14719053"}],"prism:publicationName":[{"@value":"Plant and Cell Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2008-07-14","prism:volume":"49","prism:number":"10","prism:startingPage":"1600","prism:endingPage":"1606"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://academic.oup.com/pcp/article-pdf/49/10/1600/5345875/pcn132.pdf"}],"createdAt":"2008-09-05","modifiedAt":"2019-02-14","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Bacterial","dc:title":"DNA, Bacterial"},{"@id":"https://cir.nii.ac.jp/all?q=Light%20Signal%20Transduction","dc:title":"Light Signal Transduction"},{"@id":"https://cir.nii.ac.jp/all?q=Flavoproteins","dc:title":"Flavoproteins"},{"@id":"https://cir.nii.ac.jp/all?q=Light","dc:title":"Light"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Complementation%20Test","dc:title":"Genetic Complementation Test"},{"@id":"https://cir.nii.ac.jp/all?q=Synechocystis","dc:title":"Synechocystis"},{"@id":"https://cir.nii.ac.jp/all?q=Photoreceptors,%20Microbial","dc:title":"Photoreceptors, Microbial"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Structure,%20Tertiary","dc:title":"Protein Structure, Tertiary"},{"@id":"https://cir.nii.ac.jp/all?q=Methionine","dc:title":"Methionine"},{"@id":"https://cir.nii.ac.jp/all?q=Bacterial%20Proteins","dc:title":"Bacterial Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Spectroscopy,%20Fourier%20Transform%20Infrared","dc:title":"Spectroscopy, Fourier Transform Infrared"},{"@id":"https://cir.nii.ac.jp/all?q=Mutagenesis,%20Site-Directed","dc:title":"Mutagenesis, Site-Directed"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282810694946432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694)."}]},{"@id":"https://cir.nii.ac.jp/crid/1050298201110969472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Time-resolved study on signaling pathway of photoactivated adenylate cyclase and its nonlinear optical response"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215498231424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215819476480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cyanobacterial monogalactosyldiacylglycerol-synthesis pathway is involved in normal unsaturation of galactolipids and low-temperature adaptation of Synechocystis sp. PCC 6803"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232146599680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234738508160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A PixD—PapB Chimeric Protein Reveals the Function of the BLUF Domain C-Terminal α-Helices for Light Signal Transduction"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168888778240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Selective Photoinduced Dimerization and Slow Recovery of a BLUF Domain of EB1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706518552064","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Seeing the light with BLUF proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708142388992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Blue Light-Mediated Manipulation of Transcription Factor Activity <i>In Vivo</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708536190592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Differentiation of photocycle characteristics of flavin-binding BLUF domains of α- and β-subunits of photoactivated adenylyl cyclase of Euglena gracilis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182101317760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cross‐linking analysis reveals the putative dimer structure of the cyanobacterial BLUF photoreceptor PixD"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184693817088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Genetics of the Blue Light-Dependent Signal Cascade That Controls Phototaxis in the Cyanobacterium<i>Synechocystis</i>sp. PCC6803"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576215860293504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Slow Conformational Changes of Blue Light Sensor BLUF Proteins in Milliseconds"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584339772577792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of N‐ and C‐terminal regions on oligomeric formation of blue light sensor protein <scp>SyPixD</scp>"}]},{"@id":"https://cir.nii.ac.jp/crid/1390569711987676928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"<i>In vivo</i> localization and oligomerization of PixD and PixE for controlling phototaxis in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803"}]},{"@id":"https://cir.nii.ac.jp/crid/2051714792003842688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enhanced conformational fluctuations during protein reactions"}]},{"@id":"https://cir.nii.ac.jp/crid/2120307889534151296","@type":"OtherWorks","resourceType":"学術雑誌論文(journal article)","relationType":["isIdenticalTo"],"jpcoar:relatedTitle":[{"@value":"Crucial role in light signal transduction for the conserved Met93 of the BLUF protein PixD/Slr1694"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1093/pcp/pcn132"},{"@type":"OPENAIRE","@value":"doi_dedup___::2e75bd2a41ce157073d224ef3563e1d8"},{"@type":"IRDB","@value":"oai:irdb.nii.ac.jp:00897:0004023984_isIdenticalTo_DOI_PQSMemkwx4wn95oSRRqAkoFOJ2l"},{"@type":"CROSSREF","@value":"10.1007/s12551-017-0355-6_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1016/j.febslet.2011.02.003_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcs108_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1016/j.bbalip.2013.12.007_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1021/acs.jpcb.1c10100_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1007/s12551-017-0258-6_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1039/c0pp00130a_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1016/j.jmb.2011.04.032_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1016/j.febslet.2015.05.019_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcw218_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1021/jacs.1c13121_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1002/pro.4658_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1016/j.jbc.2023.105285_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1101/2021.12.13.472511_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.2323/jgam.2020.06.001_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1246/cl.190195_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"},{"@type":"CROSSREF","@value":"10.1021/cb400174d_references_DOI_JcV01WU06mpIHsxyLJmDGg8lw4N"}]}