{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1361699993852860672.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.1110716108"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.1110716108"}}],"dc:title":[{"@value":"Microbial methane production in oxygenated water column of an oligotrophic lake"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            The prevailing paradigm in aquatic science is that microbial methanogenesis happens primarily in anoxic environments. Here, we used multiple complementary approaches to show that microbial methane production could and did occur in the well-oxygenated water column of an oligotrophic lake (Lake Stechlin, Germany). Oversaturation of methane was repeatedly recorded in the well-oxygenated upper 10 m of the water column, and the methane maxima coincided with oxygen oversaturation at 6 m. Laboratory incubations of unamended epilimnetic lake water and inoculations of photoautotrophs with a lake-enrichment culture both led to methane production even in the presence of oxygen, and the production was not affected by the addition of inorganic phosphate or methylated compounds. Methane production was also detected by in-lake incubations of lake water, and the highest production rate was 1.8–2.4 nM⋅h\n            <jats:sup>−1</jats:sup>\n            at 6 m, which could explain 33–44% of the observed ambient methane accumulation in the same month. Temporal and spatial uncoupling between methanogenesis and methanotrophy was supported by field and laboratory measurements, which also helped explain the oversaturation of methane in the upper water column. Potentially methanogenic Archaea were detected in situ in the oxygenated, methane-rich epilimnion, and their attachment to photoautotrophs might allow for anaerobic growth and direct transfer of substrates for methane production. Specific PCR on mRNA of the\n            <jats:italic>methyl coenzyme M reductase A</jats:italic>\n            gene revealed active methanogenesis. Microbial methane production in oxygenated water represents a hitherto overlooked source of methane and can be important for carbon cycling in the aquatic environments and water to air methane flux.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380013170916734212","@type":"Researcher","foaf:name":[{"@value":"Hans-Peter Grossart"}],"jpcoar:affiliationName":[{"@value":"Department of Limnology of Stratified Lakes, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, D-16775 Stechlin, Germany;"},{"@value":"Institute for Biochemistry and Biology, University of Potsdam, D-14469 Potsdam, Germany;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170916734208","@type":"Researcher","foaf:name":[{"@value":"Katharina Frindte"}],"jpcoar:affiliationName":[{"@value":"Department of Limnology of Stratified Lakes, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, D-16775 Stechlin, Germany;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170916734209","@type":"Researcher","foaf:name":[{"@value":"Claudia Dziallas"}],"jpcoar:affiliationName":[{"@value":"Department of Limnology of Stratified Lakes, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, D-16775 Stechlin, Germany;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170916734210","@type":"Researcher","foaf:name":[{"@value":"Werner Eckert"}],"jpcoar:affiliationName":[{"@value":"Israel Oceanographic and Limnological Research, The Yigal Allon Kinneret Limnological Laboratory, Migdal 14650, Israel; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013170916734211","@type":"Researcher","foaf:name":[{"@value":"Kam W. Tang"}],"jpcoar:affiliationName":[{"@value":"Virginia Institute of Marine Science, College of William & Mary, Gloucester Point, VA 23062"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2011-11-16","prism:volume":"108","prism:number":"49","prism:startingPage":"19657","prism:endingPage":"19661"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.1110716108"}],"createdAt":"2011-11-17","modifiedAt":"2022-06-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335813521792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of interannual variation in winter vertical mixing on CH_4 dynamics in a subtropical reservoir"},{"@value":"Effect of interannual variation in winter vertical mixing on CH<sub>4</sub> dynamics in a subtropical reservoir"}]},{"@id":"https://cir.nii.ac.jp/crid/1050289874222464768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Linking Stoichiometric Organic Carbon–Nitrogen Relationships to planktonic Cyanobacteria and Subsurface Methane Maximum in Deep Freshwater Lakes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168750766080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Assessing Geochemical Bioenergetics and Microbial Metabolisms at Three Terrestrial Sites of Serpentinization: The Tablelands (NL, CAN), The Cedars (CA, USA), and Aqua de Ney (CA, USA)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360568467234080256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Aerobic methane production by planktonic microbes in lakes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846642710984576","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Methylocaldum marinum sp. nov., a thermotolerant, methane-oxidizing bacterium isolated from marine sediments, and emended description of the genus Methylocaldum"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846644378661760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biogeochemical Typing of Paddy Field by a Data-Driven Approach Revealing Sub-Systems within a Complex Environment - A Pipeline to Filtrate, Organize and Frame Massive Dataset from Multi-Omics Analyses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567543993728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Temporal and spatial variations in methane emissions from the littoral zone of a shallow mid-latitude lake with steady methane bubble emission areas"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707371078400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The composition of organic phosphorus in a river during the springtime irrigation period"}]},{"@id":"https://cir.nii.ac.jp/crid/1390853334128933248","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Meteorological Control of Subtropical South American Methane Emissions Estimated from GOSAT Observations"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151842060379776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Detection of planktonic coenzyme factor 430 in a freshwater lake : small-scale analysis for probing archaeal methanogenesis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.1110716108"},{"@type":"CROSSREF","@value":"10.1029/2019jg005542_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1186/s40645-021-00450-7_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.2151/sola.2021-037_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1002/2015jg002972_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1016/j.scitotenv.2019.133916_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1099/ijs.0.063503-0_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0110723_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1016/j.agrformet.2020.108184_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.1016/j.ejrh.2022.101238_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"},{"@type":"CROSSREF","@value":"10.3390/w12020402_references_DOI_LwL8J89zM8Et6EiOstQUrFRFfhB"}]}